资源描述：
《Antibodies for immunosensors》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、ANALYTICACHIMICAACTAELSEVIERAnalyticaChimicaActa347(1997)177-I86AntibodiesforimmunosensorsAreviewBet-toldHock*TechnicalUniversityofMiinchenatWeihenstephan,FacultyqfAgricultureundHorticulture,DepartmentqfBotany,D-85350Freising,GermanyReceived12September1996:receivedinrevis
2、edform4February1997:accepted14February1997AbstractImmunosensors(1%)areminiaturizedmeasuringdevices,whichselectivelydetecttheirtargetsbymeansofantibodies(Abs)andprovideconcentration-dependentsignals.Abbindingleadstoavariationinopticalproperties,electriccharge,mass,orheat,w
3、hichcanbedetecteddirectlyorindirectlybyavarietyoftransducers.ThequalityofISsprimarilydependsupontheselectivityandaffinityoftheAbsusedinthereceptorunit.Sincesensitivityisdirectlyrelatedtotheaffinityoftheligandbinding,reversibilityexcludeshighsensitivity.Consequently,detect
4、ionunitsforsingle-useandquasi-continuousmeasuringdevicesarepresentlypreferred.Animportantfuturedevelopmentisseeninthefieldofmultianalytesensors.ThemostattractiveapplicationisbasedonAbswithsufficientlydifferentcross-reactivities.Theresponsepatternprovidesinformationonthepr
5、esenceandconcentrationofstructurallysimilaranalytes,astheyarefoundforinstanceinpesticidegroupssuchasthes-triazines.ItisobviousthatthisdevelopmentwillbesignificantlyacceleratedbyapplyingrecombinanttechniquesforAbproduction.Thenextstepisdirectedtowardthegenerationofrecombin
6、antAblibraries,whichwillprovideahugerepertoire,generatedattheDNAlevel,fornewAbtypes.Keywords:Immunosensors;Immunoassays;Multianalytesystem;Recombinantantibodies;Pesticides1.IntroductionThisreviewisintendedtoshedsomelightonthepresentsituationof1%byanalyzingthepossibilities
7、Immunosensors(1%)areconsideredamajordevel-aswellasthelimitationsofIStechnology.Itisshownopmentintheimmunochemicalfieldandconnectedthatthemainlimitationisrelatedtotheantibodieswithhighexpectations.Thistechnologyhasbeen(Abs)andtheirproperties.Whereastheshortageofextensively
8、reviewed,e.g.[l-5].However,thereAbsislikelytobeabolishedbyrecombinanttechni-appearsaconsiderable
