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1、《EnglishforBiologyStudents》1001班【32】Rachel(涂瑞倩)《EnglishforBiologyStudents》,atfirstglance,It'saprettydauntingsubject.However,undertheleadershipoftheteacherZhang,weenjoyedit!Now,gobackandhavealookatthewaywehavegothrought,butfindwithlove.Tobeginningwiththe"fear"andthen"relieved"
2、,butendwith"love".《EnglishforBiologyStudents》broughtmealotoffun,sentimentandhelp.TeacherZhangnotonlygiveustheprofessionalEnglishknowledge,what'smore,healsogaveusalotofknowledgethoseoutsideoftextbooks.Heisanexcellentprofessionalteacher,Hemademeunderstandthoughexcellentprofessi
3、onallevelisimportant,comprehensiveabilityandbroadenourknowledgeisindispensable.Hismanyrichinphilosophy,stillsovividinmymind!“Summaryortheabbreviation"chooseonefromthetwotodoaslastjobabouttheBiologicalprofessionalEnglish.OriginallyIthought:abbreviation.ReadanEnglishliterature,
4、andthenalittleunderstanding,abbreviationswouldnotbeaproblem.However,Istillwanttochallengemyself.Notseekingcomparedwithothers,buttosurpassmyself!SodecisivelyIchoosealargerreviewofthe"risk"--summary.Fortunately,sophomoreyear,IservedastheMinisterofScienceandTechnologyofLifeScien
5、ce,Ihavearchivedpapersofallresearchprojects.Ofcourse,thesepapersofaresearchprojectsIwouldn'trumor.IonlyreferencethemtopracticemyEnglishskills.ThesiteandthemechanismofoxidationofArgininekinaseAbstractArgininekinase(argininekinase,AK)(EC2.7.3.3)isaphosphodiesteraseguanidineskin
6、ase,composedof403aminoacids,whichcontainsfivecysteines(Cys23,Cys127,Cys139,Cys201,Cys271),theexperimentwefoundthattheoxidationstatebandandrestorethestatewiththephenomenonofelectrophoresisAK,andthroughaseriesofbiotechnologymeans,respectively,ofthesefivesitesCysmutatedtoserine(
7、Ser),toidentifyoxidationsitesandtostudytheoxidationmechanism,andbyUVfluorescence,respectively,toexplorethestructureafterthemutationofthetheAKvarioussitesrelatedtoactivity.Keywords:Argininekinase,rite-directedmutation,oxidationsites,SDS-PAGEIntroduction1.Argininekinase[1](Argi
8、ninekinaseAK)(EC2.7.3.3)isakinaseofaphosphorylatedoriginalguanidinoc