海带多酚的分离提取及其抗肿瘤活性研究

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1、海带多酚的分离提取及其抗肿瘤潘陛研究IsolationofphlorotanninsfromLaminariajaponicaAreschandtheirbioactivitiesofantitumorAbstractKelpiSonecommonkindofbrownalgaalongDaliancoast．Theyareabundantinbioactiveconstituents，whichmakethempotentialresourcesforadvancedpharmaeeuticalutilization．Inordertom

2、akethebestuseofkelp，thepresentreacherisfocuseonthehighthroughputscreeningforthepurposeofantiturnoractivities．Theresultsareasfollows：1、Ethanolextractionmethodisoptimisedbysingle．factorstest．nleresultsshowedthatahighyeildofthecrudephlorotanninsof24．25mg·k91Wasobtainedunderth

3、econditionsofpH10，extractedtwicewi廿180％ethanol-aqueous，thendeclouredbyCHClsby7h．Thenabstractedbyethylacetate，theoptimisedconditiondeterminedbysingle-factorstestistheequalvolumeethylacetateextractinpH3．4twice，30minonce．Theyeildisabout10．3mg·kg～．2、Fourmethodsincludingethan01

4、extraction，Soxhletrefluxing，ultrasound—assistedandmicrowave—assistedextractionsarecompared．Microwave．assistedextractioniSprovedtobethebestmethodwhichyieldis52．5％higherthanthatofethanolextractionorSoxhletrefluxingandhigherthanultrasonic-assistedby150．5％．Thebestconditionsofm

5、icrowaveextractionoptimizedbyorthogonaltestareasfollows：pHis7，intermediatefire，90s，ratioofsolidtoliquoris1：10．Theyeildis34．52mg·k91．4、111eextractisfurtherdepuratedbysephadexcolumnc11romatographyG-25whichiselutedwithdeionizedwater．Theflowrateiscontrolledat60mL／h．ThreegrouDS

6、Canbeseperatedforuntreatedkelpandtwogroupsfortreatedkelp．5、Theeffectofcrudeextractliquoronthreecancercells(HeLacells、MCF一7cells、PC一12cells)isexaminedbyM，丌assay．TheIC50ofcrudephlorotanninsonHeLacellsis2．32lxg·mL—andMCF一7cellsorPCI2cellsissuddenlykilled．IC50ofrefinedphlorota

7、nninsonHeLacells，MCF一7cellsandPCI2cellare0．2111．tg·mL一、O．174pg·mL～、O．15899·mL_respectively．Theexhibitionontumorcellsofgroup1ofuntreatedkelpiS72．41％at1．45p分mL一．Theexhibitionontumorcellsofgroup1oftreatedkelpismorethan90％atO．30斗g·mL-1and90％forthegroup2at1。68gg·mL一．KeyWords：ph

8、lorotannins；extract；microwave—assistedextraction；antitumoractivities；大连理工大学硕士研究生学位论文大连理工大