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时间:2019-05-07
《氰虫酰胺对小菜蛾生物学特性及相关酶活性的影响_英文》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、昆虫学报ActaEntomologicaSinica,July2014,57(7):815-823ISSN0454-6296EffectsofcyantraniliproleonbiologicalcharacteristicsandtherelatedenzymeactivitiesinPlutellaxylostella(Lepidoptera:Plutellidae)LIUXia,WANGHong-Yan2,XIAXiao-Ming1,QIAOKang1,WANGKai-Yun1,*(1.CollegeofPlant
2、Protection,ShandongAgriculturalUniversity,Tai’an,Shandong271018,China;2.CottonResearchCenter,ShandongAcademyofAgriculturalSciences,Jinan250100,China)Abstract:【Aim】Thediamondbackmoth(DBM),Plutellaxylostella(L.),isoneofthemostimportantpestsofcruciferousplantsinthewo
3、rld.Cyantraniliproleisanovelanthranilicdiamideinsecticideregisteredforthecontroloflepidopteranpests,butthereissparseinformationavailableregardingitseffectsonP.xylostella.Here,westudiedtheeffectsofcyantraniliproleonbiologicalcharacteristicsandtherelatedenzymeactivi
4、tiesofP.xylostella.【Methods】Theleaf-dippingmethodwasusedtotestthetoxicityofcyantraniliproletothe3rdinstarlarvaeofP.xylostella,andtheLC20andLC50valuesweredetermined.The3rdinstarlarvaeweretransferredontocabbageleavessoakedinLC20andLC50ofcyantraniliprolesolution[20%l
5、ethalconcentration(LC20)=0.03mg/Land50%lethalconcentration(LC50)=0.08mg/L]andallowedtofeedfor48h,thenthebiologicalcharacteristicssuchasthepupalduration,pupationrate,adultemergencerateandsoonwererecorded,andtherelatedenzymeactivitieswerealsomeasured.【Results】Theres
6、ultsshowedthattheestimatedLC20andLC50valuesofcyantraniliproleonthe3rdinstarlarvaeinleafdipbioassaywere0.03mg/Land0.08mg/L,respectively.CyantraniliproleatLC20andLC50concentrations(LC20=0.03mg/L,LC50=0.08mg/L)couldextendthedurationofthe4thinstarandpupalstage,andredu
7、cethepupalweightandfecundityofP.xylostella.Intreatedgroups,thepupationrate(F=42.19;df=2,6;P<0.05)andadultemergencerate(F=13.15;df=2,6;P=0.006)weresignificantlylowerthanthoseinthecontrolgroup,respectively.Cyantraniliproleexposurealsosignificantlyincreasedtheactivit
8、iesofprotectiveenzymes(CATandPOD)at12haftertreatment,butcausednoeffectontheactivitiesofdetoxificationenzymes(CarE:P=0.43;GST:P=0.54;ODM:P=0.25).Incontra
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