淋巴瘤中rassf5a基因启动子区甲基化状态与mrna表达的研究

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1、授予单位代码10089学号或申请号20122300HebeiMedicalUniversity硕士学位论文科学学位淋巴瘤中RASSF5A基因启动子区甲基化状态与mRNA表达的研究研究生：王连静导师：刘丽宏教授郭炜教授专业：内科学二级学院：第四医院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研宄所获得的研宄成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归

2、河北医科大学所有。否则，承担相应法律责任。.....•：；�、导师签章-级赛fe领导盖普？河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除1文rb特别加以标注和致谢等由芒从t中不包含其他人已经发表或撰二>教师对此进行了审定。木论文由本人独立撰写，文责自负。研究生签名：导师签章年$月）々日目录中文摘要·················································································1英文摘要··································

3、···············································4英文缩写·················································································8研究论文淋巴瘤中RASSF5A基因启动子区甲基化状态与mRNA表达的状态前言·················································································9材料与方法································

4、······································10结果···············································································19附图···············································································22附表···············································································23讨论

5、···············································································31结论···············································································34参考文献·········································································34综述RASSF基因家族在肿瘤中的研究进展····························

6、·······37致谢·····················································································53个人简历···············································································54中文摘要淋巴瘤中RASSF5A的基因启动子区甲基化状态与mRNA表达的研究摘要目的：淋巴瘤是十大恶性肿瘤之一，在每年新诊断的血液系统肿瘤中约占一半，且发病率每年呈上升趋势。按细胞来源淋巴瘤分为B细胞淋巴瘤和T细胞淋巴

7、瘤，B细胞淋巴瘤中以弥漫大B细胞淋巴瘤最为多见。淋巴瘤起病的症状较为多样，多以无痛性进行性淋巴结肿大、局部肿块及相应器官压迫症状为最初表现，侵袭性较高的类型淋巴瘤进展迅速，一部分经联合化疗可持续缓解，但多数患者的治疗疗效不佳，预后较差。因此关注淋巴瘤病因学研究、早期诊断及靶向治疗十分必要。随着对肿瘤研究的不断深入，发现除了基因突变外，表观遗传学机制在肿瘤的发生及发展中也起着重要的作用。DNA甲基化是表观遗传学重要的组成部分，且DNA甲基化具有可逆性的特点，所以DNA甲基化是目前肿瘤相关研究中的热点。RA