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1、PRODUCTINFORMATIONANDMANUALQuantoBioInc.3/F,IPEBuilding2,1DishengEastRoad,Yizhuang,Beijing,China100176Tel:+861059773899Fax:+861059773998Email:info@quantobio.comMouseTNF-αELISAKitCatalog#1910341:1plate1910342:2plates1910345:5plates1910349:CustomsizeEnzyme-linkedimmunosorbentassay(ELIS
2、A)forthequantitativedetectionofmouseTumorNecrosisFactorAlpha(TNF-α)incellculturesupernatant,serum,andplasma.Pleasereadentirepackageinsertcompletelybeforeusingtheproduct.Forresearchuseonly.Notfordiagnosticortherapeuticprocedures.MouseTNF-αTABLEOFINTRODUCTION1CONTENTSINTENDEDUSE…………………
3、……………………………1BACKGROUNDINFORMATION…………………………..1PRINCIPLEOFTHEASSAY………………………………...2MATERIALSANDMETHODS3REAGENTSPROVIDED……………………………………..3OTHERSUPPLIESREQUIRED…………………………….4SAFETY………………………………………………………..4PRECAUTIONSFORUSE…………………………………..4SAMPLEPREPARATION………………..……………….....5REAGENTPREPARATI
4、ON………………………………….5ASSAYPROCEDURE………………………………………..8ASSAYPROCEDURESUMMARY…………………………9CALCULATIONOFRESULTS………………………………10TYPICALDATA……………………………………………….10PERFORMANCEOFCHARACTERISTICS11SPECIFICITY………………………………………………….11SENSITIVITY…………………………………………………11RECOVERY…………………………………………………...11PARALLELIS
5、M…………………………………………….....11LINEARITYOFDILUTION…………….……………………..12PRECISION…………………………………………………...12REFERENCES…………………………………………….....13INTRODUCTIONINTENDEDTheQuantoBiomouseTNF-αELISAkitisdesignedtodetectUSEandquantifythelevelofmouseTNF-αincellculturesupernatant,serum,andplasma.Thiskitisfor
6、researchuseonlyandnotintendedfordiagnosticpurposes.BACKGROUNDTumorNecrosisFactor-a(TNF-α),alsoknownascachectin,INFORMATIONplaysacentralroleininflammationandapoptosis.TNF-αisproducedbymanyactivatedcelltypesincludingmonocytes,macrophages,astrocytes,TandBlymphocytes,NKcells,keratinocyte
7、s,granulocytes,fibroblasts,andcertaintumorcells.TNF-αisamultifunctionalcytokineinvolvedinmanydifferentpathways,inhomeostasisandpathophysiologyofmammals.Inresponsetoinflammatorystimulation,macrophageormonocytesecretesTNF-αthatcaninduceapoptoticornecroticcelldeathofcertaintumorcellline
8、s.Inaddition
