基于微量试剂分配控制的微操作方法和实验-研究

《基于微量试剂分配控制的微操作方法和实验-研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、哈尔滨工业大学工学硕士学位论文AbstractComparedwithgravity,adhesionforcedominatesinthemicrongradeobjectoperationduetosizeeffectandsurfaceeffect.Howtopickupaccuratelyandreleasereliablebecomecriticalissues,andthisproblemincreaseswiththereducingofobjectsize.Duetothemechanicalcontactbetweenthegripper

2、andthemicroobjects,ahighlocalmechanicalstresscouldoccurandthemicroobjectsareeasilytobedamagedwhenmechanicaltweezersandvacuumtoolsareapplied.Thereforeitisnecessarytoinvestigatenewharmlessoperationmethod.Basedontracesofreagentdispensingcontrol,anewmicromanipulationmethodwasproposed.

3、Theliquidbridgeformedbetweendropandmicroobjectcouldbeadjustedbymanipulatingthevolumeofdrop,andundertheeffectofcapillaryforcethemicroobjectwaspickedup.Byejectingouttracevolumeofdropthereleasingprocessofmicroobjectrealized.First,byanalyzingtypicalcapillaryforcemodelbetweensolidandli

4、quidinterfaces,theformulaofcapillaryforcewithinliquidbridgeformedbetweenreagentinthecapillarytubeandmicroobjectwasderived.Thefactorsaffectingthecapillaryforceandthefeasibilityofmicromanipulationbycontrollingcapillaryforcewerediscussed.Themicromanipulationmethodbasedontracesofreage

5、ntdispensingcontrolwasproposed.Pressureadjustmentofreagentatcapillaryorificewasusedtocontrolthemeniscus’svolumetopickupmicroobjectandpiezoelectricceramicsschemewasputforwardtoejecttracesofreagentdroptoreleasemicroobject.Theworkingprinciplesofthesetwoschemeswereanalysed.Bydividingt

6、heoperationprocessintovariousstages,relatedoperationstrategieswerepresented.Second,accordingtopressureadjustmentschemetocontrolthevolumeofmeniscustopickupmicroobject,Fluentsoftwarewasusedtosimulatetheinfluenceofpressureoncapillarymeniscus.Basedonpiezoelectricschemeaboutejectingout

7、tracesofreagentdroptoreleasemicroobject,thesimulationwasanalysedundertwodifferentsignals:DC(DirectCurrent)voltageandrectangularpulsevoltage.UnderDCvoltagesignalthedeformationofcapillarywallswereacquiredbyAnsyssoftwareandthesedeformationdatawereimportedtoFluenttosimulatetheappearan

8、ceofcapillarymenisci.Underrectang