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1、宁夏医科大学硕士研究生学位论文摘要TheprotectiveeffectsofLBPonfocalischemiccerebralinjuryinmicebyattenuatingthemitochondrialapoptosispathwayABSTRACTObject:ToinvestigatetheneuroprotectiveofLyciumbarbarumpolysaccharide(LBP)onfocalcerebralischemicinjuryinmiceandtoexploreitspossiblem
2、echanism.Methods:MaleICRmicewereusedtomakethemodelofmiddlecerebralarteryocclusion(MCAO)afterintragastricadministrationwithLBP(10,20and40mg/kg)andNimodipine(4mg/kg)forsevensuccessivedays.After24hofreperfusion,neurologicalscoreswereestimatedandinfarctvolumeswereme
3、asuredby2,3,5-triphenyltetrazoliumchloride(TTC)staining.Morphologicalchangesinischemicbrainswereperformedforhematoxylin-eosin(HE)staining.ThenumberofapoptoticneuronswasdetectedbyTUNELstaining.ActivationofCaspase-3proteininischemicbrainswasmeasuredbyspectrophotom
4、etry.TheBax,Bcl-2proteinexpressionandCytC,Caspase-3,-9andcleavedPARP-1activationwereinvestigatedbyimmunofluorescenceandwestern-blotanalysis.Results:Comparetothevehiclegroup,LBP(20and40mg/kg)groupssignificantlyreducedinfractvolumeandneurologicaldeficitscores(P<0.
5、05);comparetothevehiclegroup,LBP(10,20and40mg/kg)groupsrelievedneuronalmorphologicaldamagerespectivelyandalsoobviouslyattenuatedtheneuronalapoptosis(P<0.05).After24hofreperfusion,Comparetosham-operatedgroup,theactivationofCaspase-3wasincreasedsignificantlyinvehi
6、clegroup(P<0.01),whileLBP(40mg/kg)groupandnimodipine(4mg/kg)groupcouldmarkedlyreducedCaspase-3activityinischemicbrain(P<0.05).Intheresultsofimmunofluorescencetests,after24hofreperfusion,theexpressionofBax,CytCandCaspase-3proteinsweresignificantlyincreasedintheve
7、hiclegroup(P<0.01)andBcl-2proteinexpressionwasmarkedlylowerthansham-operatedgroup(P<0.01).TheexpressionofBax,CytCandCaspase-3proteinswereobviouslydecreased(P<0.01)andBcl-2expressionwasmarkedlyIII万方数据宁夏医科大学硕士研究生学位论文摘要increased(P<0.05)inLBP40mg/kgandnimodipinegrou
8、ps.InWesternblotanalysisresults,expressionofBax,CytC,Caspase-3,-9andcleavedPARP-1proteinsweresignificantlyincreasedinvehiclegroupmiceischemicbrainscomparedtosham-oper
