敌草隆免疫胶体金快速检测试纸条的开发

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1、一ABSTRACTInthispaper,thecolloidalgoldimmunochromatographicrapidteststripwasdevelopedforthedeterminationofDiuronresidues，whichwaseffectivelyappliedtoidentifytraceDiuroninwatersample．TheDiuroncompetedwithDiuron—BSAconjpgateimmobilizedonthestripstocombinewiththecolloidalgold-

2、labeledanti．Dim'onpolyclonalantibodies．Anti．DiuronpolyclonalantibodieswerepurifiedbyproteinA—sepharose4BandKLH．sepharose4B．Goldchloridewasreducedwithsodiumcitrateandsodiumborohydridetomake17amand6．56ni／lcolloidalgolds，whichWascoupledwiththeanti．Diuronpolyclonalantibodiesas

3、immuno-colloidalgoldafteridentificationbytransmissionelectronicmicroscope．Thehapten-BSAandgoatanti—rabbitIgGweredispensedontest．1ineandcontrollinerespectivelyandthepolyclonalantibodywassolidifiedtoglassfiberstoprepareimmunochromatographicstrips．Thedetectionmethodofindirect

4、competitionWasestablished：gold—labeledantibodieswerecombaniedcompetitivelybyDiuronandDiuron-BSAcoatedwithsolidphasecarrierfilm．Diuronresiduelevelsi1"1samplescallberapidlydeterminedbyobservation．Thecoatedantigen,dilutionfoldofthegold．1abeledantibodiesandtheGoatanti—rabbitan

5、tibodywerestandedat0．24Pg．1：2and1：500astheoptimumconditions，respectively．ThelimitofdetectionofDiuronteststripwas5ng／mL．TheestablishedmethodWasvalidatedbytheinstrumentalmethodofHPLC．Fifteenwatersamples，determinedtobefreeoftheanalyte，werespikedatthreeconcentrationsof5，20，100

6、ng／mL．Afterfilteredby0．22pmmembrane，thesamplesweredirectlyusedforanalysisbyHPLC．TheresultsobtainedbyteststripwerecoherencewiththeresultsobtainedbyHPLC．TheresultsindicatedthattheteststripsCanbeappliedintherapiddetectionofDiuronresidues．TheinterferencewiththedetectionofDiuro

7、nresiduescausedbythestructuralanaloguecompoundsWasstudied．WhentheconcentrationofChlorbromuron，Linuron，Thidiazron，Monuron，andMonolinuronWas1000ng／mL，theeffectofCTOSS．reactivitycouldbeignored，whichvalidatedthespecificityofthetest．Theprocessofdetectionissimple，rapidandconveni

8、ent，whichcouldbefinishedwithin10minutes．Theresultscarlbevisuallyreadbyeyeswithoutinstrume