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ID:33738384
大小:2.13 MB
页数:56页
时间:2019-02-28
《无菌大鼠的人工培育与生物学特性的测定仙台病毒胶体金免疫层析方法的建立》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国医学科学院&北京协和医学院硕士学位论文ABSTRACTRatisakirldofwidelyusedlaborato叮锄imal,锄dge加fke朔tis锄adv孤ltagestool嬲ith勰nobacteria.Withthequalityofexperimental锄imalshavebecOmeincreasin91ydemanding,large·scaleuseof10calizationgellIl矗.eeratsbecomeatrend.Inresponseto廿lis仃end,wesuccess向
2、lIyproduced廿1egernlf沁emtsontheb懿isofprcVious、Vork.IIlthisexperiment,wehadhysterectomysu唱eD,ontwopregn锄trats锄dobtained36角talm戗锄dl8ofthemweresurvive.Thesurvival豫tewas50%.A船r2monthsofage,廿lesexualmaturi够oftlleF0generationofgem丘.ce馏tswerematedbetvveenbrother加dsister
3、,卸dwe90tF1genemtionoftherats;111es锄eway’wegotF2generation.TIl蜘wedete订IliIledtllebloodcount,bloodbiochemis仃y,咒immuneindicators锄do唱锄coe侬cientof5一Week-old锄d8一week-oldgem舶erats.Theseindicatorswillprovideagoodb觞icdateforUle印pIicationofgem舶erats.Sendaivimsisarcspirato
4、叮Virus,.tc锄c锄∞佗spi豫t0呵diseasesofrodents粕ditmustbeexcludedontllecle锄animaI锄dhi曲erleVel锄imals.Thisstudyaimst0establishacolloidalgoldimmunochroma幻gra【phyinViewofitsspecific,∞nsitiVeandrapid.WegottIle∞ndaiVims矗.0malIantoicfluidofchick锄b巧。舳dpurifiedthe锄tig朗byuln?acen
5、n.ifhgation.We90tthecolloidalgoldparticlesofl0肿觚dl6nmusingt锄nicacid—sodiumcitmtereductionmethod.Andmet、I旧l【indofparticlesrcspectiVelyco硒ugatedthcsecond锄tibodyofmice锄dmts.The嘶psshouldbe觞SemblyaRerthegoldstandardp嬲finished:ni打ocellulo∞membmneswerecoatcdwith锄tigen0
6、f∞ndaivimsasateStlineandthefirStantibodyofmice锄drats髂thecontrolline,锄dthena900dabsorbentp印erw邪adhesiVcd0nthetopof协eNCmembme、ⅣhenthegoldstaIldardpad粕ds锄plepadwe心adhesiVedbelowtheNCmemb啪e.AfbcrVerifiedwitllpositiVe∞mm,nleTline锄dClinewe他botllcoloredtored.The仃ialpre
7、limina拶establishedacoIloidalgoldimmunochromatography硒rthedetection0fsendaiVims,锄dthismethodh勰acenaincomp撕ngsignifica办cc觚dprOspect.Keyword:Grem毹e胁;HyStere=c;tomysu学巧;B100dco眦;Bloodbioch锄i嘟;PT;Immuneindicators;OI?gancoefficient;Sendaivirus;Colloidalgold;lmmunochro
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9、【rips3中国医学科学院&北京协和医学院硕士学位论文第一部分无菌大鼠的人工培育及生物学特性的测定引言实验动物学伴随着生物医学在长期的动物实验过程中逐步形成,尤其是实验科学的兴起,进一步推动了实验动物科学的发展。许多生物医学的重大发现都与实验动物有关。自本世纪以来,实验动物学由于新技术的应用得到迅速发展,规
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