欢迎来到天天文库
浏览记录
ID:33445357
大小:3.97 MB
页数:37页
时间:2019-02-26
《出核因子crm1和her-2neu在胃癌组织中的表达及临床意义》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、出核因子ORIII和HER-2/neu在胃癌组织中的表达及临床意义中文摘要目的:探讨胃癌及癌旁正常组织中出核因子CRMI蛋白表达和人表皮生长因子受体2HER-2/neu基因状态与胃癌临床病理参数之间的关系,分析它们之间的相关性,探讨CRMI和HER一2/neu在胃癌中的应用价值。方法:分别用免疫组织化学(IHC)方法和荧光原位杂交(FISH)方法,检测135例胃癌患者术后癌组织及癌旁组织CRMI蛋白表达和HER-2/neu基因扩增状态,结合临床病理资料和对术后患者的随访观察结果进行统计学分析。结果:CRMI主要表达于细胞核及
2、胞浆。CPu~11在135例胃癌组织中的阳性表达率显著高于癌旁正常组织(57.8%VS6.7%,P<.0001)。CRMI高表达与患者性别(P>O.05)、年龄(P>O.05)、肿瘤分化程度(P>0.05)均无相关性,而与肿瘤大小(P3、1i.85%(16/135),而在癌旁正常组织中未见扩增。其扩增与肿瘤分化程度(P0.05)。结论:在胃癌中,CRMI蛋白表达显著高于癌旁正常组织,多因素生存分析显示:CRMI高表达是胃癌术后生存时间的独立预后因子,提示预后不良。HER一2/neu基因在胃癌中的扩增比例较高,且与CRMI表达有一定的相关性:HER一2/neu扩增的患者CRMI呈高表达。因此联合检测胃癌组织中CRMI和HER-2/neu的表达情4、况有助于正确评价胃癌的生物学行为,判断胃癌的恶性程度,为胃癌的临床治疗提供新线索和依据。关键词:CRMI:HER-2/neu;胃癌;预后研究生:孙笑笑(肿瘤学)导师:邱文生(教授)IIIIIlllllllllUJlllllllllllllJllllllllllllllllllIY2588985ExpressionofCRMlandHer-2/neuandTheirClinicalSignificanceinGastricCancerAbstractObjects:Theaimofthestudywastoevaluateth5、eexpressionlevelsofCRM1andHer-2/neuingastriccancersandthecorrespondingnormaltissues,correlationwiththeirclinicopathologicalparametersandtherelationshipofCRM1andHer-2/neuinthemetastasisandoccurrencegastriccarcinoma.Methods:135postoperativepatientswereexaminedforCRMl6、expressionbyimmunohistochemistryandforHer-2/neuexpressionbyfluorescenceinsituhybridization(FISH).Results:ThepositiveexpressionrateofCRMlinneoplastictissuesandadjacentgastricmucosawere57.8%and6.7%respectively.CRMllevelswerelinkedtomoreadvancedtumorstages,poorprognos7、is,andlikelihoodofrecurrence(P<0.05).TheCoxmultivariateanalysisindicatedthattheCRM1expressionwasanindependentindicatorfortumorrecurrence(P--0.0048).16ofthe135gastriccancerspecimens(11.85%)werepositiveforHer-2/neu,theexpressionswerenotfoundinpairednormaltumor-adjace8、nt.PositivecasesforHer-2/neualsohadhi【ghCRM1expressionlevels俾--0.0104).Conclusion:ThecurrentresultssuggestthatCRM1isover-expressedingastficcarcin
3、1i.85%(16/135),而在癌旁正常组织中未见扩增。其扩增与肿瘤分化程度(P0.05)。结论:在胃癌中,CRMI蛋白表达显著高于癌旁正常组织,多因素生存分析显示:CRMI高表达是胃癌术后生存时间的独立预后因子,提示预后不良。HER一2/neu基因在胃癌中的扩增比例较高,且与CRMI表达有一定的相关性:HER一2/neu扩增的患者CRMI呈高表达。因此联合检测胃癌组织中CRMI和HER-2/neu的表达情
4、况有助于正确评价胃癌的生物学行为,判断胃癌的恶性程度,为胃癌的临床治疗提供新线索和依据。关键词:CRMI:HER-2/neu;胃癌;预后研究生:孙笑笑(肿瘤学)导师:邱文生(教授)IIIIIlllllllllUJlllllllllllllJllllllllllllllllllIY2588985ExpressionofCRMlandHer-2/neuandTheirClinicalSignificanceinGastricCancerAbstractObjects:Theaimofthestudywastoevaluateth
5、eexpressionlevelsofCRM1andHer-2/neuingastriccancersandthecorrespondingnormaltissues,correlationwiththeirclinicopathologicalparametersandtherelationshipofCRM1andHer-2/neuinthemetastasisandoccurrencegastriccarcinoma.Methods:135postoperativepatientswereexaminedforCRMl
6、expressionbyimmunohistochemistryandforHer-2/neuexpressionbyfluorescenceinsituhybridization(FISH).Results:ThepositiveexpressionrateofCRMlinneoplastictissuesandadjacentgastricmucosawere57.8%and6.7%respectively.CRMllevelswerelinkedtomoreadvancedtumorstages,poorprognos
7、is,andlikelihoodofrecurrence(P<0.05).TheCoxmultivariateanalysisindicatedthattheCRM1expressionwasanindependentindicatorfortumorrecurrence(P--0.0048).16ofthe135gastriccancerspecimens(11.85%)werepositiveforHer-2/neu,theexpressionswerenotfoundinpairednormaltumor-adjace
8、nt.PositivecasesforHer-2/neualsohadhi【ghCRM1expressionlevels俾--0.0104).Conclusion:ThecurrentresultssuggestthatCRM1isover-expressedingastficcarcin
此文档下载收益归作者所有