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ID:33365743
大小:10.66 MB
页数:51页
时间:2019-02-25
《基于液滴微流控的肿瘤血管生成模拟系统的建立》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据采用建立的液滴控制系统在芯片上连续形成300nl的液滴200个,液滴内细胞计数结果显示,细胞数目与理论值(390个)一致。阵列形成实验显示,在芯片上可稳定形成4X8液滴阵列。2、应用微阵列液滴分析药物抗血管生成作用通过液滴阵列分析药物Vatalanib在不同浓度下的抗血管生成的作用。结果显示,随着药物浓度增加,内皮细胞管状形成能力发生变化,药物浓度至20uM时,血管生成收受到明显的抑制,表现为成管面积显著降低。提示该阵列系统可实现多条件下的同时分析检测。3.应用自动化平移台系统建立共培养液滴阵列为进一步探讨自动化控制上述系统的可行性,将该系统与X—Y—Z自动化平移台集成控制,结果显示在
2、自动化控制下,可连续完成肿瘤细胞液滴形成、内皮细胞液滴形成及液滴融合等过程,为进一步实现自动化、高通量肿瘤血管生成的分析检测奠定了基础。结论构建了基于液滴微流控的阵列式细胞共培养系统,成功实现了肿瘤血管生成过程的体外模拟,为高通量进行肿瘤血管生成的机制研究及相关药物筛选提供了新的分析平台.关键词液滴微流控芯片,人脐静脉内皮细胞,细胞共培养,肿瘤血管生成5万方数据英文论著摘要Establishmentofadroplet-·basedmicrofluidicCO—-culturesystemformimickingtumoranPrefaceglogeneslsMetastasisisoneof
3、thebiologicalcharacteristicsofmalignanttumors.Itisaclinicalproblem,whichistheleadingcauseofdeathinpatients.Studieshaveshownthattumorgrowth,invasion,andmetastasisdependonangiogenesis.Therefore,theresearchontumorangiogenesis,especiallythenewtumorbloodvessels,havegreatsignificanceinexploringthemechanis
4、moftumormetastasis,screeningofantitumordrug,guidingofclinicaltreatment.Nowadayshasestablishedawidevarietyoftumorangiogenesisexperimentmodelandevaluationstandard,includingbodymodel,invitromodelandmicrofluidicchipmodel,buttherearecertaindrawbacks.Theinvivomodelsbasedonanimalexperimentsaretime-consumin
5、g,highlycost,andnotmimicthemicroenvironmentwellinvivo.Transwellchamberisanwidelyusedinvitromodelincurrent,itCannotwellsimulatethemicroenvironmentinvivoandhavemoredifficulttoachievehigh-throughputassay.MicrofluidicchipsCanreducesampleconsumptionandmodeltestingcosts,andCansimulatethemicroenvironmentin
6、vivotosomeextent,butmicrofluidicsystemshavebeendevelopedforhigh—throughputanalysisofthestillrelativelycumbersomeoperation,anddifficultcontrolproblems.Therefore,itisparticularlyimportanttoestablishabeaerinvitromodeltosimulatetumorangiogenesisinvivotumormicroenviroument.Dropletmicrofluidicsisdeveloped
7、inmicrofluidictechnology,basedonanewtechnologyCanformandmanipulatetinyvolumesofliquidthroughthereactionsystemtoachievehighthroughputandcellwrappedintinydropletsinseparate,low,interferencedetectiontarg
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