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1、AbstractWiththedevelopmentofMicro-Electro-MechanicalSystem(MEMS),dropletmicrofluidicsystemhasbeenwidelyusedinchemicalanalysis,bio-pharmaceuticals,DNAamplificationandcellanalysis.Theapplicationofdroplet-basedsystemhasarequirementthatthedimension，speed,concentr
2、ationofdropletshouldbecontrolledinaccuracybecauseoftherequireofexperimentalconditionsanddropletdetectionisanurgentneed.Inthispaper,basedontheoverviewofexistingdropletdetectionmethods,dropletdetectionbasedonimageprocessingandtheprincipleofcapacitancearepropose
3、d.Thepurposeistodesignamicrofluidicsystemwhichisportable,convenientdetection,accuracycontrol.Theadvantageofdropletdetectionmethodisnoneedtoaddmarkers,highsensitivity,suitableforfieldtesting.Themicrofluidicsystemincludesdropletgenerationsystemanddigitalmicrosc
4、opesystem.DropletgenerationsystemismadeupofNitrogencylinder,micropumps,micro-chip,dropletcollectiondeviceandsomeotherdevicesifnecessary.Dropletgenerationsystemisusedfordropletgeneration.Thegenerateddropletsareusedfordropletdetectionexperiments.Digitalmicrosco
5、pesystemconsistsofmicroscope,CCD(ChargeCoupledDevice)cameraandmonitorofdigitalmicroscopesystem.Digitalmicroscopesystemisappliedtoobservethedropletdevelopment,acquiredropletimagesandprocessdropletimages.Inthispaper,dropletdimensiondetectionbasedonimageprocessi
6、ngisdescribed.DropletimagesareacquiredbyacommonCCDcamerabecausethemicrofluidicsystemisusedinfieldtest(Nonlaboratoryconditions).DropletimageshaveamotionblurbecausetheexposuretimeofcommonCCDcameraistoolongformotiondropletsinthemicrochip.Motiondeblurringisanindi
7、spensablepartfordropletdimension,shapeandvolumedetectionbyimageprocessingsinordertoimprovethedetectionprecision.Thefirststepofimageprocessingisthemotiondeblurringafterimagedenoising.Themotionblurisremovedbymotiondeblurring.Secondly,thedropletimageisdividedint
8、othetargetareaandthebackgroundareabyimagethresholdsegmentation.Thirdly,targetinformationofthedropletimageisextractedthroughregionofinterestextraction.Finally,theshapeanddimensionareobtain
