基于凝胶的液滴微流控-研究及应用

《基于凝胶的液滴微流控-研究及应用》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、摘要我们的微泵将驱动模块放在微流控芯片外，便于维修与更换，延长了微泵以及芯片的使用寿命。4．实现基于琼脂糖液滴微流控的PCR扩增。基于上述液滴产生原理，我们将DNA模板以及PcR反应试剂封装在琼脂糖微液滴内，在片外收集后进行PCR扩增，并成功获得了扩增产物。这证明了以琼脂糖液滴为微反应器实现高通量实验反应的可行性。关键词：琼脂糖，微流控，液滴，聚二甲基硅氧烷，微阀HAbs昀ctAbs仃actDropl武-baScdmicrofluidicsutiUzesmeshe撕ngf．orcebetw嘲twoir

2、rⅢliscibkph够es(aqⅦ暑ousphaseandoilph硒e)togenerateuniforIn-size，pic01i仃e—volumedrople协inmicroch呦elwimf诧quencyashighasmousandsofherz．Ast11edropletsareseparatedbyoilphase，eachofnlemcanben弓atedasaIlind印endentreactor，muSrernarkablyincreasingmetotalreactionmro

3、ughput．Droplet．basedmicrofluidicsispromi咖ginfieldssuchasgenescreeniIl舀diseasedetection，directedenzymeev01udon，dmgscreen协g，ma钯rialsvnmesisandmicro喝tmc眦fabrication．HoweV％delicleneiesstillcXistindroplet．basedmicroflllldics．First硝’an，commonaqueousdropletste

4、ndtocoalesce，whichmakes10n争timereservationaJldfollow-up仃eatmentsdi伍culttoachieve．Second，itishardtoaddorrcmoveaspecificcompositionofmedropIetreactor．comparedtocommonaqueouspbase，ag盯osesol砸oneXh_ibitsatemperanJre—sensi咖e‘sol-to唔el’characteristic．So，ontheo

5、nehand，1argemoleculescanbeenc印sulatediIlsidemeagarosegeldrople协byshnplyc001ing，whichbringsgreatconveniencefDrs越lplereservationandsubseque芏lta删ySes，0nmeo如erhalld，lli曲一Ⅱlr．oughput西situHlicro—s砸lcturefabricationcallbeachievedinsidemicmfluidiccha皿els埘mthech

6、angeoftemperature．h1addidon，ag咖segelhasaporouss协lcture，smanmoleculescanmoveacrossmedr叩letsthroughosmosis，whichsimpli6edexperimentaloperationandchjpdesign．Accordingtomea：bovebackground，basicprinciplesofagarose．b鹊eddropletmicronuidicsarefirstlydiscussed．T

7、hen，a11i11-simsynthesizableone一、张yagaroseballvalves扛ucnH．eandamiero．pumpsyStemafeizlllovative】ypfoposedbasedon出eseprinciples，achie诎lgnuidsflowingre则ationandrcagentsiIl仃I)ductionreSpectively．Atlast，thefjaSibilityofPCRamplificationinsideagarosedropletsisp

8、rovedillmispaper．Themamcontentsoft11iswork证cludemef0UowillgaspectS：1．DevelDparapidnlabPDMSmicro丑uidicchipfabrjc确ngme也od．‘So矗lithography’isusedtof孙ricateSU·8maledie，femaledieofthechipisacquiredthrough‘molding’，thenirreversiblebond