番茄红素对脑缺血再灌注大鼠氧化应激与缺氧损伤的影响

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1、ABSTRACTObjective：StrokeisOtlUol'theseverestcRtls,eg,ofdelbmfityanddeathinhumanAgreatdealofstudiesi}／dicatethatcaddadveslrcssistheimporlantcausativefactorsofneurontraumaaliert：erebmlischemia-reperfusioninjuryLycopeneisoneoftheantioxidantslndietAceumulatiingevidencesstronglysuggestthatly

2、copenehastheprotoctioneffi．-ctofcerebrovasculardiseases，humanstudiesshowthatserum[ycopeneisnegativerelatedwithcardio—cerebrovasculardiseasesThisstudyaimedtoinvestigatetheprotectioneffectsoflycopeneorlcerebralischemia—roperfusioninjuryinducedbyfocalcerebralisdlemialnratsinoxidativestress

3、andanoxicwaysITheestablishmentofMiddlecerebralarteryocclusion(MCAO)20maleSDratswei苎htin9260～2809weredividedrandomlytowelghcintoA．B，C，Dgroup．eachgrouphas5ratsThediameterofembolismthreadeachgroupwcFe0,22mm，0．24ram，0．26mmand0．28ramThemodelofcerebralischemia—reperfusioninjurywasestablishedb

4、ymiddlecerebralarteryocclusion(MCAO)Findingthebefittingdiameterofembolismthreadaccordingtothesuccessfulratesofeachgroup2Theeffectsoflycopeneoncerebralischemia-reperfusioninjuryinratsinoxidativestressandanoxicdamage64maleSDratsweighting130-1409wereacclimatizedfor3dayspriortobein—gdivided

5、randomlytowei曲tinto5groups：LPH(16)LPL(16)，Model(16)'SHAM(10)andNor(6)groupRatsofLPH，LPLgroupsweregivenlycopeneorally20，5mpel(gbwperday．andModel，SHAMratswere弹venequdsaladodforl5dThertcerebralischemia-reperfusioninjurywitsestablishedbymiddlecerebralarteryocclusi011fMCAO)inLPHIPLantlModelg

6、roup．SHAMgroupwas¨sedHsfhkcsurgerycontrolThereperfusionwas2hafterisehemia24hafterreperfilsion、tbeneurologicalinfarctionsizesweremeasuredandneurologicaldelicilsevaluatedbyamethodofgradingonascaleof0-8at3、24hafterreperf_LIsionTheactivitiesofSOD、CAT、NOS、lNOS、LDH．thecontentsofMDA、NO、ROS，LDi

7、ncodexofbrainandsel．1lmuricacidweremeasuredat24hafterreperfusionThelevelsofBcl一2mRNAandtifF—ltlmRNAincortexandhippocampiweredeterminedbyusingreversetranscri-prioripolymerasechainreaction(aT-PCR)techniqueResult：lSucccsslhlrateofB口'oupanddeathrateofDgrouporethehighest；failurerate