红景天苷对缺血再灌注大鼠脑损伤和lps致pc12细胞损伤的保护作用的分析

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1、●英文摘要ProtectiveeffectsandmechanismsofSalidrosideagainstcerebralischemia—reperfusioninjuryinratandLPS—inducedcellulardeathinPC12Abstract111eroleofinflammationandimmuneresponsefollowingcerebralischemiainbrainischemicinjuryhasbeenwidelyinvestigatedinthelastdecade．Salidros

2、ide，anovelextractofthechineseherbCrassulaceaehastheactivityofneuroprotectionaftercerebralischemia-injury．However,theeffectsofsalidrosideontheinflammatoryresponseandtheexpressionoftumornecrosisfactor-alpha(TNF·a)followingcerebralischemiaremainedunclear．硼1epurposesofthep

3、resentstudyweretoevaluatetheeffectsofsalidrosideoninflammationandtheexpressionofTNF-afollowingcerebralischemia．Furthermore，wetestedtheeffectofsalidrosideonLPS．inducedcellulardeathinPC12cellsbasedonMTTassay．PartlAIMT0studytheprotectiveeffectsandthemechanismsofsalidrosid

4、eagainstcerebralischemia-reperfusioninjuryinMCAO—I／Rrats．METHEDSFocalcerebralischemiainratsWasproducedby1．5hocclusionofthemiddlecerebralartery(MCAO)and24hreperfusion．Salidroside(5mg‘kg叶)wasadministeredintravenouslyimmediatelyafterocclusionandreperfusionrespectively．The

5、neurologicdeficitscorewasinvestigatedaccordingtoZea-Longa’SStandard．Theinfarctvolumewasassessedwiths01．areImagePro4．5after1vrCstaining．TheactivitiesofMPOwereestimatedbytestboxesproducedbyNanjingJianchengBioengineeringInstitute．ThelevelsofTNF—Qincentralneutralsystemwere

6、measuredbywesternblot．RESULTSCompared谢tllthemodelgroup，salidrosidetreatmentcallsignificantlydecreasetheneurologicdeficitscore(P<0．05)，reducethemoisturecapacity(尸如．05)andtheinfarctvolume(P<0．05)．AsignificantincreaseinMPOactivitiesWasfoundinthesalidrosidetreatmentgroup，c

7、ompared诵tllthemodelcontrolgroup(P≮O．01)．Also，salidrosidetreatmentdecreasedtheoverexpressionofTNF—Q俨<0．05)inthebrain．CONCLUSIONSalidrosidehasaprotectiveeffectOilcerebralischemia-reperfusioninjuryandthiseffectiSlikelyrelatedtoTNF—Q．4■英文摘要Part2AlMTostudytheeffectofsalidro

8、sideoncellulardeathinlipopolysaccharideinducedPC12cells．METHEDSPC12cellsweretreatedwithLPSorLPSplussalidroside．TIlevi