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ID:32739333
大小:2.07 MB
页数:33页
时间:2019-02-15
《bfgf联合骨髓间充质干细胞促进周围神经再生的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、THEEXPERIM匣NTALSTUDYOFbF(讯JOINTM匣SENCHYMAI,STEMCELLSFORPROMOTINGPERIPHERALNERVEI也(逦NERATIONResearchorientation:bonesurgeryPostgraduatestudent:LiDaweiGraduatestudenttutor:LiBingshengABSTRACTObjective:Toobservethebasicfibroblastgrowthfactorjointmesenchymalstemcell
2、sinducedperipheralnerveregenerationrolefortheclinicalencountercasesofperipheralnervedefectstof'mdawayofconvenientandfeasiblerestoration.Methods:1、MSCscollection,trainingWeuseketaminetoanesthetizeaWistarrats,andcutapartlateralSkillofthighinsterileconditions,strip
3、pingoutthefemur,andwashmarrowcavityrepeatly、7I,imheparinphysiologicalsaline,thenfluidintothetube,mixandcentrifuge,washtwicebyD-Hanksbalancedsaltsolution,andthencentrifugeagain,jointhebasisofvaccinationintheculturemediumbottleat37℃,volumeWasO.05ofC02saturationhum
4、idityfortraining.Replacebalancedsaltsolutionafterthreedays,removecellsonthebottomforevery3daysoftime,tobecovered而mculturedcellsatthebottomofbottlesafterusingtrypsintodigest,andthenjointheserummediumforterminationofdigestion,jointheappropriatemediumcount,theninoc
5、ulateinthenewculturebottle.CountingoftherequiredamountofMSCs(morethan2X107),putthecellsintoliquidnitrogentopreserve.2、animalexperimentWistarmts30,male,200garound,randomlydividedintoA,B,C3groups,each、析血10.Anesthetizebyketamineinabdominal,revealleRlegsciaticnervei
6、nsterileconditions,cutapartthenerveinthepiriformismarginofabout3mm,whichisthesciaticnervestemabout6mm,retractastheirnatural.Operationwillbeunderthemicroscopeoftherespectiveendsofthesiliconesetsofpeoplewithin1.0mln,8/0non-invasivesutureclosureoftheoutermembranean
7、dsiliconewallSOthatmakingthetwoendsofthesiliconetubefrom10mm.GroupA:putMSCs+bFGFsolution(concentrationofabout2×107/m1)intosiliconetube;BGroup:putMSCssolution(concentrationofabout2×107/m1)intosiliconetube;CGroup:siliconetubefilledwithnormalsaline;threegroupswereu
8、singfibringlueclosedinterface,suturewounds.analysisofresults1、generalobservation:MSCs+bFGFgroup,onlyoneratlimbsfoothasulcersandedema,mostoftheothertwogroupsareemergin
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