新生期芬太尼镇痛对内脏痛觉高敏感幼鼠内脏痛敏感性与脊髓fos蛋白表达的影响

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1、45mmHg、60mmHg、75mmHg时没有交互作用p>0．05)。③造模可使幼鼠腰骶段脊髓背角FLI细胞数和FLI细胞积分光密度值显著增高，新生期CI前预防性腹腔注射芬太尼镇痛，可使幼鼠腰骶段脊髓背角FLI细胞数和FLI细胞积分光密度值显著减低：差别均具有统计学意义ff=13．76，P<0．05；F=78．58，P<0．001)。两者存在交互作用。结论新生期持续CI可造成幼鼠痛阈下降，出现慢性内脏高敏感性，且这种高敏感性能持续到幼年期，为幼鼠内脏高敏性模型。新生期CI前预防性腹腔注射芬太尼，能够导致脊髓神经元Fos蛋白高表达下降，阻止幼鼠脊髓初

2、级中枢敏化，抑制幼鼠慢性内脏痛觉高敏感形成。关键词新生期芬太尼镇痛内脏痛觉高敏感脊髓Fos蛋白EffectofNeonatalFentanylAnalgesiaonVisceralPainSensitivityandSpinalFosExpressioninVisceralHyperalgesiaRatsObjectiveToexploretheeffectofFentanylanalgesiainneonatalperiodonvisceralsensibilityofpaininvisceralhyperalgesiaratsbythemeth

3、odofbehaviorandelectrophysiologyresearch．Observeingthec-fosexpressionoflumbosacralspinaldorsalhornindevelopingrats．ToinvestigatethemechanismofblockingvisceralhyperalgesiabyFentanylanalgesiainneonatalperiod．Thisstudycanprovidethetheoreticalbasisoftreatmentandpreventfortheabdomi

4、nalpainrelatedtofunctionalgastrointestinaldiseaseinchildren．Method(1_-)Accordingtothefactorialdesign，32SDratsweredividedintofourgroups谢tlleach8，GroupAiBl。establishthemodelofvisceralhyperalgesiaindevelopmentalrats，andintraperitonealinjectFentanylbeforecolorectalirritation(cDsti

5、mulationinneonatalperiod，GroupAlB2：．establishthemodelofvisceralhyperalgesiaindevelopmentalrats，butAdministrationsalinebeforeneonatalCI，GroupA2Bl：notimposedonCIatneonatalperiodand，andintraperitonealinjectFentanyl，GroupA2BE：notimposedonCIatneonatalperiod，butAdministrationsaline．

6、Ratsinthemodelinggroupweretreated、)l，ithcolorectalirritationonceadayduring7consecutivedaysfromthe8t11dayafterbirth，thecontrolratswerenotexcepthandledinthesameway．Conventionallybreedingtotheyoungperiod(6一weekage)，observingthescoreofAbdominalWithdrawalReflex、visceralpainthreshol

7、dandmeasurementofelectricaldischargeofexternalobliquemuscleofabdomenunderdifferentpressuresofcolorectaldistensionirritation(CRD)toevaluatevisceralalgesthesiainrats．②32SDratsweretreated晰thasabove，．Onthe6thweek,ratsweresampledthespinalcordfromL6toS2，thesemi-quantityanalysisofsta

8、iningdensityandthecellnumbersofFos·LikeImmunoreactivityofspin