欢迎来到天天文库
浏览记录
ID:32187550
大小:1.67 MB
页数:38页
时间:2019-02-01
《螺内酯对醛固酮诱导的人脐静脉内皮细胞某某某-1+m某n某表达的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、安徽医科大学硕士学位论文吸光度值与内参β-actin条带的吸光度值之比作为目标mRNA的表达量。结果:①实验重复三次,体外培养的脐静脉内皮细胞存活良好,以0.4%的台盼兰染液染色后计数,细胞存活率约为96%-98%。②与阴性对照组比较一定浓度醛固酮可以明显上调脐静脉内皮细胞表面LOX-1mRNA的表达P<0.05差异有统计学意义。③醛固酮在一定的浓度范围内呈剂量依耐性上调体外培养的脐静脉内皮细胞LOX-1mRNA表达,即LOX-1mRNA表达量B2、较一定浓度的螺内酯可以有效阻止醛固酮诱导的LOX-1mRNA表达上调,P<0.05差异有统计学意义,⑤螺内酯组及聚肌苷酸组与阴性对照组比较,P>0.05无统计学意义。⑥螺内酯组与多聚肌苷酸组相比P>0.05差异无统计学意义。(D组与A组相比较P值为0.001,D组与E、F组比较P值分别为0.001及0.002,A组与E、F组相比P值分别为0.584及0.951,E、F之间比较P值为0.544,)结论:螺内酯可以下调醛固酮诱导的体外培养脐静脉细胞LOX-1mRNA的表达,可能具有抗氧化应激、抗动脉粥样硬化的3、作用。关键词:螺内酯;血凝素样氧化低密度脂蛋白受体-1;醛固酮;脐静脉内皮细胞4安徽医科大学硕士学位论文EffectofspironolactoneonLectin-likeoxidizedlowdensitylipoproteinreceptor1(LOX-1)mRNAexpressioninHUVECsinducedbyaldosteroneAbstractObjective:Weculturedhumanumbilicalveinendothelialcells,treatedthemwithspi4、rolactone(Spiro)andtheChemicalantagonistofLOX-1polyinosinicacids(POLYI),andthenstimulatedwithAldosterone(ALD).ObservedthedifferenceofthemRNAexpressionofLectin-likeoxidizedlowdensitylipoproteinreceptor1(LOX-1)amongthesixgroups.ToestablishtheeffectsofSpiroo5、nthemRNAexpressionofLOX-1inhumanumbilicalveinendothelialcells,andfurtherinvestigatethiseffectofprotectinganddelayingsomeAtheroscleroticdiseases.Methods:Theexperimentwasaccomplishedbetween2011/5-2011/10inthethecenterlaboratoryoftheFirstAffiliatedHospitalof6、AnhuiMedicalUniversity.①humanumbilicalveinendothelialcellswerepurchasedfromNanjingKaiJibiologicalscienceandtechnologydevelopmentCo,LTDandthenculturedin1640supplementedwith20%(v/v)fetalbovineserum.②Thehumanumbilicalveinendothelialcellswereculturedfor48hour7、sin1640with20%(v/v)fetalbovineserum,,andthenchangedtheculturemediumto1640withoutfetalbovineserum,thenSub-confluentcellshavingstarvedin1640containing0.0%FBSfor12h.③After12hTheculturedhumanumbilicalveinendothelialcellsweredividedintosixgroups:thenegativecon8、trolgroup(groupA),treatedwithdifferentvariousconcentrationsofaldosteronegroup(10nmol/L、100nmol/L、1000nmol/L,groupB、C、D),theALD+polyinosinicacid(thechemicalinhibitorsofLOX-l)group(groupE),theALD+Spirogroup(groupF),Gr
2、较一定浓度的螺内酯可以有效阻止醛固酮诱导的LOX-1mRNA表达上调,P<0.05差异有统计学意义,⑤螺内酯组及聚肌苷酸组与阴性对照组比较,P>0.05无统计学意义。⑥螺内酯组与多聚肌苷酸组相比P>0.05差异无统计学意义。(D组与A组相比较P值为0.001,D组与E、F组比较P值分别为0.001及0.002,A组与E、F组相比P值分别为0.584及0.951,E、F之间比较P值为0.544,)结论:螺内酯可以下调醛固酮诱导的体外培养脐静脉细胞LOX-1mRNA的表达,可能具有抗氧化应激、抗动脉粥样硬化的
3、作用。关键词:螺内酯;血凝素样氧化低密度脂蛋白受体-1;醛固酮;脐静脉内皮细胞4安徽医科大学硕士学位论文EffectofspironolactoneonLectin-likeoxidizedlowdensitylipoproteinreceptor1(LOX-1)mRNAexpressioninHUVECsinducedbyaldosteroneAbstractObjective:Weculturedhumanumbilicalveinendothelialcells,treatedthemwithspi
4、rolactone(Spiro)andtheChemicalantagonistofLOX-1polyinosinicacids(POLYI),andthenstimulatedwithAldosterone(ALD).ObservedthedifferenceofthemRNAexpressionofLectin-likeoxidizedlowdensitylipoproteinreceptor1(LOX-1)amongthesixgroups.ToestablishtheeffectsofSpiroo
5、nthemRNAexpressionofLOX-1inhumanumbilicalveinendothelialcells,andfurtherinvestigatethiseffectofprotectinganddelayingsomeAtheroscleroticdiseases.Methods:Theexperimentwasaccomplishedbetween2011/5-2011/10inthethecenterlaboratoryoftheFirstAffiliatedHospitalof
6、AnhuiMedicalUniversity.①humanumbilicalveinendothelialcellswerepurchasedfromNanjingKaiJibiologicalscienceandtechnologydevelopmentCo,LTDandthenculturedin1640supplementedwith20%(v/v)fetalbovineserum.②Thehumanumbilicalveinendothelialcellswereculturedfor48hour
7、sin1640with20%(v/v)fetalbovineserum,,andthenchangedtheculturemediumto1640withoutfetalbovineserum,thenSub-confluentcellshavingstarvedin1640containing0.0%FBSfor12h.③After12hTheculturedhumanumbilicalveinendothelialcellsweredividedintosixgroups:thenegativecon
8、trolgroup(groupA),treatedwithdifferentvariousconcentrationsofaldosteronegroup(10nmol/L、100nmol/L、1000nmol/L,groupB、C、D),theALD+polyinosinicacid(thechemicalinhibitorsofLOX-l)group(groupE),theALD+Spirogroup(groupF),Gr
此文档下载收益归作者所有