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1、ProtocolofthepolymerasechainreactionfordetectinggeneticallymodifiedcomponentsinmaizeT~d$iB.BXj#r#r$iEfB#3;f~&HE03.1RSBBtransnene#@N*%&A%N,%NSi-F~fbillofllhc~sfi~DNA#9l~~~&jirp%AA&,~$!W@~~+~~~~,~@~SC$I~Wl~%~B~?$:E~3.2Bbl#&JJi$!polymerasechainreaction,PCR&&~~J?~'JYii%!~%l~?k&~~@~&DNA@fiH*M
2、#S&HE&&{$T*@&&&BPJg2-t&1W+%~14@3)9II%8$5DNAH%#L&fiiW-BX$bIP9rJ2+BA~#X%6,f&#&DNAReB$~~8lTIJA~~~4~~#s4!(dNTP)%~&~~t&51~l~~~Mt$*~G~;f;II&~~~~%Ai#1~t~~-m6~@&rNtmsa~~L~nl~~~v49-3.3HMl%3.31PCRpolymerasechainreaction,j%j%PCR.33.2DNA,deoxyribonucleicacid,%&&#+&@.333dNTP,deoxyribonucIeos~detr~pho
3、sph~te.H#g@f('Z@@.33.4ATPPIdeoxyadeNOSineti.iphospl~~te,~~IB~@@3.3.5dCTP,deoxycyt~dlnetriphosph~te,a$f.fi#Z#@a33.6dtiTP,deoxyguaNOSinetriphoupliate,n#!&P583@mZll.33.7dTTP,deoxythym~dinetr1plrosphate,jttlffw8:ii@@~'33.8dUTP,deoxyurid~netriphosl)hate,MRtiirtfZ#@3.3.9bplbasepair,&4iH.3.3.10
4、EDTAIethylenediaminetetraaceticacidtZ,Z@~Z,~.3.3.11TaglThermu~aquaticu,&!&%#!%.3.3.12Trisltris(hydroxymethyl)aminomethanetFi(~I&)$lBfe$2,3.3.13TEITris-C1,'EDTA##a,3.3.14IVR~invertaselgenefrommaize,3*&@fiBl%m.3.3.15Zeinlh*l#@biPBSB$,3.3.16CaMV35S135Spromoterfromcauliflowermosaicvirus,fi?g
5、#%awJi$t#J355a3.3.3.17NPTIIlneomycin-3'-phosphotransferasegenet%#*-3'-@@8@&H.3.3.18NOS1terminatorofnopalinesynthasegene,861813U&9ElSm&&k+.3.3.19IVS21intronfromthemaizealcoholdehydrogenasegene,3#B)rB#%#%ElW&BJfiE2.3.320PATtphosphinothricinacetyltranrferasegene*PTf#Z,B%##Sfl.3.3.21HSP~O;O.
6、8Kbintronfromthehsp70gene(heat-shockprotein),AQ%#j&@Q(hsp70)S$1BO0.8KbLBk4LX.3.3-22CryIA(b)1asyntheticgeneencodesthefirst648aminoa~ids~insecticidal-activeftruncatedproductidenticaltothatofCrylA(b)geneofBacillusthuringisnsi,subsp,%Z&~~fi~~&e~crylA(b)Sa.3.3.23CDPKIcalcium-dependentproteink
7、inase(CDPK)gene,$5%$&%&#@S$1.3.3.24Cry9CIinsecticidal-activeftruncatedproductidenticaltothatofCrgCgeneofBacillusthuringiensissubsp,~Z&i$~fi~&&~~l&lCrgCsm.3.3.25CaMV35SI135sterminatorofCaMVgenomcrtE~#i%Ri-~~SHl~35s%kF.3.3.26Pactin1Ithepromoterderivedfromtherice(Oryzasativa
