钙调神经磷酸酶与锌指转录因子在大鼠钙化主动脉中的作用

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1、英文摘要eventOreverseitsdevelopmentwillhavegreatclinicalsignificance．Vascularcalcificationoncewasthoughtpassivedepositionofsupersaturatedcalciumandphosphateinthevesselwall，itwasprovedrecentdecadestobeanactiveregulatedprocessinvolvingmultiplecellsandmolecules，resemblingosteogenesis．Calcineurin(CaN)isaser

2、ine／threonineproteinphosphataseandknownastheonlyphosphatasethatregulatedbyCa升andcalmodulin．CaNisexpressedmainlyincytoplasmanddistributedwidelyineukaryotictissues．CaNinvolvesincellfunctionregulationofmultiplecelltypesbydephosphorylatingitssubstratessuchasmemberofNFATs(nuclearfactorofactivatedTcell)fa

3、milyandtranspositionfromcytoplasmtOnucleus．EvidencesshowthatCaNandGATA一6playimportantrolesinmyocardialhypertrophyandbonemetabolismrecentyears．CaNandGATA一6notonlypromoteosteoblastdifferentiationandboneformation，butalsotheyarerequiredforthegenesisofosteoclast．WhetherCaNandGATA-6playanroleinvascularcal

4、cificationisnotyetknown，andnoreporthasbeenseenatpresent．ThisstudyiStoconstructaratvacularcalcificationmodelbysubcutaneouslyinjectionofwarfarinandVitaminD3，furthertodetectCaNmRNAandproteinexpressionaswellasenzymeactivityofCaN．FurthermoretoknowwhethercyclosporinA(CsA)，inhibitorofCaNactivity，influenceC

5、aNandGATA一6expressioninrataortaandtheextentofvascularcalcification，7英文摘要ratingroupCweretreatedwithCsA．Throughthistest，weaimtoinvestigatetheroleofCaNandGATA一6invascularcalcification，SOthatanewthoughtforclinicaltreatmentofvascularcalcificationwillbepresented．Methods：36fourweeksmaleSprague—Dawlayrats．8

6、0～909inweight，wererandomlydividedinto3groups：groupA(normalcontrolgroup)，groupB(calcificationgroup)andgroupC(CsAgroup)．Eachgroupcontain12rats．Fromday1today8duringthetest，ratsineachgroupweresubcutaneouslyinjectedofvatamink1atdoseof15mg·kg"I'd～．CsA，10mg·kgI'd1,werealsoadministeredtogroupCbyintraperiton

7、ealinjection，whilegroupAandBwereinjectedofisodosenormalsaline．Fromday3on，groupBandCweresubcutaneouslyinjectedofvataminD3atdoseof3x105U·kg-I．d卅tillday5，aswellaswarfarin(15mg·lOOg—bodyweight)every12hour