毛细管电泳-电化学检测在中药成分-分析和临床化学中的应用-研究

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1、ABSTRACTCapillaryElectrophoresis(cE)isoneoft11emostimportantseparationtechniquesinrecenttwentyyears．BecausethetheoreticalplatenmberisgreatlydeVelopedtotensofthousalldsa11devenmillions，andmevolumeofsampleinjectionreachesnallomilliliterleverinCEa11alys

2、is，ithasbeenstudiedaIldappliedtomostan“yticalareas．HoweVer，thesmalls啪pIeinjectionVoIumeandvery协incapiIIarybringaboutdif![icultiestodetection．Thecommonmemodsofdetectionareultra_Vi01et(UV)，laserinducednuorescence(LIF)，massspectra(MS)，etc．Everymemodhass

3、omeadvantagesandsu虢rsfromsomedrawbackstoo．Forexample，LIFisverysensitivebutthein㈣entationsareveryexpensive，a11dsomecomplicatedderivationproceduresareoftenneeded．E1ectrochemicaldetectionismoresensitive血anUVdetection，aIldhasmallyadvantageswhenitcombines

4、withCE，suchassimpleinstrumentationafldoperation，lowcost，wideli王1earrange，etc．SothemethodofCE—EDisextensivelystudiedandappliedinmostanalytical丘eldsfbritsaboveadV肌tages．Thegoalofmisthesisistoexploresome印plic砒ionsinphaHnaceuticalanalysis，biochemicalanal

5、ysisandfoodanalysisbyCE—ED．Thecomemsoftllismesisincludesixchapters：1．PrefaceThecharacteristicsofCE，theseparationmodels，mebaSic廿leoriesofCE，theCEdetectors，mestudiesandapplicationsofCE—EDareintmducedsimply．Thegoalandsign墒canceofthisthesisareintroducedt

6、oo．2．DeteminatioⅡofsixnaVonesin工e口胛H，‘E譬j氇PD腭缸‘瞎and工eD以脚，Hs却D腮缸l西GranulesbyC印ilIaryEIectrophoresiswithEIectrochemicaIDetectionAsimultaneousdetenninationofsixnavones，includingphloridzin，hesperedin，rutin，kaemper01，apigenin，quercatin，in三8D门“r“s√qpo"fc“s

7、andthreekindsofcommercial￡Pon“r“s。，(tpD阳】_c“sGmnulesbyC印illaryE1ectrophoresiswith2006年华东师范J==学申请硕-十：学位论文3ABSTRACTElec仃ochemicalDetection(CE—ED)isreported．Theeffectsofsevemlimportantfhct0Tssuchastheacd毋andconcet灯ationofmIlningbu仃er，s印arationVoltage，in

8、jectiontime，aIlddetcctionpotentialwereiIⅣestiga把dtoacquiretheoptimumconditions．TbeworkingelecⅡodewasa300岬dianletercarbondiscelec打odepositionedopposite也eoutletofcapillary．Theanalytescouldbewellseparated“thin20min证a75cmlen舒hcapillary砒aseparationvoltage