毛细管电泳在中药-分析中的应用-研究

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1、ApplicationsResearchofCapillaryElectrophoresisintheAnalysisofTraditionalChineseMedicineHongquanGong（MedicinalChemistry）Superior：ProfessorFuhaiWu[Abstract]Thisthesisincludesthefollowingfivechapters:Chapter1Weintroducedtheapplicationanddevelopmentofcapillaryelectrophoresisintheanal

2、ysisoftraditionalchinesemedicine，narratedthebackgroundandsignificanceofourstudy.Chapter2ToestablishamethodforthedeterminationofimperatorinandisoimperatorininRadixAngelicaedahuricaeandYuanhuZhitongPianbynon-aqueousmicelleusingcapillaryelectrokineticchromatography.Theseparationwasp

3、erformedonauncoatedfusedsilicacapillaryof55cm×75µmID(47cmeffectivelength).20mmol/LNaH2PO4+100mmol/LSDS+80%formamidebuffersolution（pH7.12）wasselectedfortherunningbuffer.Thevoltageappliedwas15kV.Thesamplewasinjectedbygravity（5s，15cm）.Thedetectionwavelengthwas254nm.Theinternalstanda

4、rdwasberberinehydrochloride.The−1linearrangesofdeterminationforimperatorinandisoimperatorinwere8.0～40.0µg·ml−1（r=0.9987）and4.0～20.0µg·ml（r=0.9990）.TheaveragerecoveryforimperatorininRadixAngelicaedahuricaewas100.1%，precisionofthemethodwas2.83%(RSD,n=6).Theaveragerecoveryforisoimpe

5、ratorininRadixAngelicaedahuricaewas102.4%，precisionofthemethodwas3.07%(RSD,n=6).TheaveragerecoveryforimperatorininYuanhuZhitongPianwas101.3%，precisionofthemethodwas2.58%(RSD,n=6).TheaveragerecoveryforisoimperatorininYuanhuZhitongPianwas100.2%，precisionofthemethodwas2.64%(RSD,n=6)

6、.Themethodprovedtobeconveient、rapidandwell·i·repeatable，andcanbeusedforquantitativedeterminationofRadixAngelicaedahuricaeandYuanhuZhitongPian.Chapter3Toestablishamethodforthedeterminationofberberinehydrochloride、palmatinehydrochloride、jatrorrhizinehydrochlorideandtetrahydropalmat

7、ineinNiantongWanbycapillaryelectrophoresis.Theseparationwasperformedonauncoatedfusedsilicacapillaryof55cm×75µmID（47cmeffectivelength）.100mmol/Lsodiumdihydrogenphosphate+50%ethanol（pH5.70）wasselectedfortherunningbuffer.Thevoltageappliedwas21kV.Thesamplewasinjectedbygravity（5s，15cm

8、）.Thedetectionwavelengthwas211nm.Theline