atp介导aktmtorp70s6k信号转导通路激活促进大鼠脊髓损伤后运动功能恢复

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1、优秀毕业论文兰州大学硕士研究生学位论文ATPmediatedAkt／mTOR／p70S6kinasesignalingpathwayactivationpromotesimprovementoflocomotorfunctionafterspinalcordinjuryinratsCandidatemaster：HULingyunTutor：WENYiminDepartmentofSpineSurgery，LanzhouGeneralHospital，LanzhouCommand，PLA，Lanzhou730050，Gansu，PR

2、ChinaAbstractObjectiveAkt／mTOPJp70S6Ksignaltransductionpathway,asacentralcontrollerofcellgrowth。proliferation，survival，anddifferentiationinresponsetoextracellularsignals，growthfactors，nutrientavailability,energystatusofthecellandstress，hasrecentlygainedattentioninneuro

3、science．Theeffectsofthissignalingpathwayontherepairofspinalcordinjury(scD，however,arenotwellelucidated．Adenosinetriphosphate(ATP)isincreasinglyrecognizedasanimportantregulatorofsignaltransductionpathways，andplayimportantrolesinfunctionalrecoveryafternervoussysteminjuri

4、es．Inthepresentstudy,weexaminedtheATP—inducedchangesoftheAkt／mTOR／p70S6KsignalingpathwayininjuredspinalcordandpotentialeffectsofthissignalingpathwayontheSCI—inducedneurologicaldysfunctionsandrepairprocessesofSCIinadultratS．MethodsSClwasproducedbyextraduralweight—dropof

5、thespinalcordusingmodifiedAllen’Sstallwithdamageenergyof50g-cmforce．Atotalof135adultfemaleSprague—Dawleyratswererandomlydividedintothefollowingfourgroups：SCIplusA『rP(n=35)，SCIplussaline(n=35)，SCIplusAlTPandrapamycin(n=35)，andsham—operated(n=32)．Usingimmunostainingstudi

6、es，Westernblotanalysesandreal-timequalitativeRT-PCRanalyses，WEdetectedthechangesofAkt，p-Akt。mTOR，p-mTOR，p70S6K，p-p70S6K，nestin，NeuN，lIl精品参考文献资料优秀毕业论文兰州大学硕士研究生学位论文NSE．NF200andGFAPexpressionsinallgroupsafterdifferentadministrationfollowingsurgery．Thespinalcordsamplestake

7、n4weeksafterdifferentdisposalinallSCIgroupswerestainedwithhematoxylinandeosin(HIE)forgeneralmorphology．LocomotorfunctionalrecoveryafterSClwasevaluatedusingtheBBBLocomotorRatingScale．ResultsThesham-operatedanimalsexhibitedlowexpressionofthesecomponentsintheAkt／mTOR／p70S

8、6KsignaltransductionpathwayattheproteinandmRNAlevel，andtheexpressionsincreasedfollowingSCI．Exogenousadministrationof心