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1、附录A英文原文LaserTissueWelding:LaserSpotSizeandBeamProfileStudiesAbstract：Thispaperevaluatestheeffectoflaserspotdiameterandbeamprofileontheshapeofthethermaldenaturationzoneproducedduringlasertissuewelding.2-cm-longfull-thicknessincisionsweremadeontheepilatedbacksofguineapigsinvivo.Indiainkwasuse
2、dasanabsorberandclampswereusedtoapposetheincisionedges.Weldingwasperformedusingcontinuous-wave1.06-m,Nd:YAGlaserradiationscannedovertheincisionstoproduce100-mspulses.Laserspotdiametersof1,2,4,and6mmwerestudied,withpowersof1,4,16,and36W,respectively.Theirradianceremainedconstantat127MonteC
3、arlosimulationswerealsoconductedtoexamine.theeffectoflaserspotsizeandbeamprofileonthedistributionofphotonsabsorbedinthetissue.Thelaserspotdiameterwasvariedfrom1to6mm.Gaussian,flat-top,dualGaussian,anddualflat-topbeamprofileswerestudied.Theexperimentalresultsshowedthat1-,2-,4-,and6-mm-diameter
4、spotsproducedthermaldenaturationtoanaveragedepthof570,970,1470,and1900m,respectively.MonteCarlosimulationsdemonstratedthatthemostuniformdistributionofphotonabsorptionisachievedusinglargediameterdualflat-topbeams.IndexTerms—Denaturation,laserbiomedicalapplications,lasermaterials-processinga
5、pplications,laserwelding,MonteCarlomethods,opticalpropagation.MATERIALSANDMETHODSA.ExperimentsInvivoweldingofskinincisionswasperformedatconstantirradiancetoinvestigatetheeffectofvariouslaserspotsizes(1-,2-,4-,and6-mm-diameterFWHM)ontheextentofthermaldenaturationattheweldsite.Adultfemaleal
6、binoguineapigs(Hartley,age7–8weeks,weight400–500grams)wereshavedthenepilatedwithachemicaldepilator(Nair,Carter-Wallace,Inc.,NewYork,NY).Eachguineapigwasanesthetizedwithatropine(0.05mg/kg),ketamine(30mg/kg),andxylazine(2mg/kg)administeredbyintraperitonealinjection.1%lidocainewith1:100000ep
7、inephrinewasusedasalocalanestheticateachincisionsite.2-cm-long,fullthicknessincisionsweremadeparalleltothespinewithano.15scalpel.Fourincisionsweremadeonthebackofeachguineapig.Approximately2–5lofIndiaink(blackIndiaRapidographink,3080-F,100-nmparticlediameter,Koh-INoo
