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1、附录A英文原文LaserTissueWelding:LaserSpotSizeandBeamProfileStudiesAbstract：Thispaperevaluatestheeffectoflaserspotdiameterandbeamprofileontheshapeofthethermaldenaturationzoneproducedduringlasertissuewelding.2-cm-longfull-thicknessincisionsweremadeontheepilatedbacksofguin
2、eapigsinvivo.Indiainkwasusedasanabsorberandclampswereusedtoapposetheincisionedges.Weldingwasperformedusingcontinuous-wave1.06-m,Nd:YAGlaserradiationscannedovertheincisionstoproduce100-mspulses.Laserspotdiametersof1,2,4,and6mmwerestudied,withpowersof1,4,16,and36W
3、,respectively.Theirradianceremainedconstantat127MonteCarlosimulationswerealsoconductedtoexamine.theeffectoflaserspotsizeandbeamprofileonthedistributionofphotonsabsorbedinthetissue.Thelaserspotdiameterwasvariedfrom1to6mm.Gaussian,flat-top,dualGaussian,anddualflat-to
4、pbeamprofileswerestudied.Theexperimentalresultsshowedthat1-,2-,4-,and6-mm-diameterspotsproducedthermaldenaturationtoanaveragedepthof570,970,1470,and1900m,respectively.MonteCarlosimulationsdemonstratedthatthemostuniformdistributionofphotonabsorptionisachievedusing
5、largediameterdualflat-topbeams.IndexTerms—Denaturation,laserbiomedicalapplications,lasermaterials-processingapplications,laserwelding,MonteCarlomethods,opticalpropagation.MATERIALSANDMETHODSA.ExperimentsInvivoweldingofskinincisionswasperformedatconstantirradiance
6、toinvestigatetheeffectofvariouslaserspotsizes(1-,2-,4-,and6-mm-diameterFWHM)ontheextentofthermaldenaturationattheweldsite.Adultfemalealbinoguineapigs(Hartley,age7–8weeks,weight400–500grams)wereshavedthenepilatedwithachemicaldepilator(Nair,Carter-Wallace,Inc.,New
7、York,NY).Eachguineapigwasanesthetizedwithatropine(0.05mg/kg),ketamine(30mg/kg),andxylazine(2mg/kg)administeredbyintraperitonealinjection.1%lidocainewith1:epinephrinewasusedasalocalanestheticateachincisionsite.2-cm-long,fullthicknessincisionsweremadeparalleltothe
8、spinewithano.15scalpel.Fourincisionsweremadeonthebackofeachguineapig.Approximately2–5lofIndiaink(blackIndiaRapidographink,3080-F,100-nmparticlediameter,Koh-INoor,Bloo
