大叶麻竹笋多糖的分离纯化和鉴定

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1、万方数据ABSTRACTr”●···一‘●1一lneseparation．puriticationProcesslngandstructualidentificationofbambooshoot(dendrocalamuslatiflorus)polVsaccharidesIVCandidate：WuJin—songAdvisor：KanJian·quan，PhDABSTRACTBambooshootsarethenewshootsofBambusoideaeandPoaceae，whichhashighfiber,lowfat．richnutrit

2、ionandisnamedas”thefirstvegetarianfood”．DendrocalamuslatiflorusisoneofthevarietiesthathavethehighestChinaproductionofbambooshootsinChina，andithashighnutritionalvalue．Abambooshootpolysaccharideisakindofphysiologicalactivitythatisextractedfrombambooofpolysaccharide．Ithasmanykindso

3、fbioactivities，suchasantitumor,antioxidant，hypoglycemicandSOon．Atpresent．therearen’tmanyresearchreportsconcerned、Ⅳithbambooshootpolysaccharidesbothathomeandabroad．Thispapertriestogaintheoptimalbambooextraction，separationandpurificationmethodsofdendrocalamuslatifloruspolysacchari

4、desandlearnmoreaboutthestructureofdendrocalamuslatifloruspolysaccharides，andthuslaysasolidfoundationforthecomprehensiveexploitationandutilizationofdendrocalamuslatiflorusbambooresources．ThisthesistakesdendrocalamuslatiflorusbambooshootsproducedinChongqingasrawmaterial，exploresth

5、eoptimalextraction，separationandpurificationprocessofbambooshootpolysacchafidesandidentifiesthestructureofthispolysaccharide．Asforextractionprocess，inordertooptimizeprocessparametersthisthesisselectsthewaterextractionandalcoholprecipitationmethodandemploystheoptimizedprocesspara

6、metersselectionoforthogonalexperiment；itchoosesultrasonicassistedextractionandoptimizationofprocessparametersandusesresponsesurface；itselectsenzyme—assistedextractionmethodandemployssinglefactorvariationmethod．Asforseparationandpurificationprocess，thisthesisselectedandcomparesdi

7、fferentdeproteinizationmethods，thewaterafterdialysisbyDEAE—Cellufose52columnchromatographyandSephadexG一50chromatographypurification，indentifiedthefinalpurityidentification．Asforstructuralcharacterization，themolecularweightV万方数据西南大学硕士学位论又ismeasuredthroughgelfiltrationchromatograp

8、hy．Thebetaeliminationreactionmethodisusedtoanal