新疆乌鲁木齐市新市区2010年房地产市场调研报告

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PipetteBoxVolumetricPipettes:Æ8–1mLÆ8–5mLÆ8–10mLGraduatedPipettes:Æ6–1mLÆ6–5mLÆ12–10mLAutomaticPipettes:Æ12–1mLautomaticpipetteandtipsPipetteBulbs:Æ14–bluepipettebulbsThin-stemmedPipettes:Æ1bagThermometers:Æ8–StickThermometersNFPAInformation:Noneneeded. DigitalThermometersØ12–DigitalThermometerBasesØ12–DigitalThermometerProbesØ3–extra9vBatteriesØ“Bad”BatteryStickersNFPAInformation:Noneneeded. GCTubÆ1–containerofseptaÆ8–workingsyringesÆ1–packageofextrasyringeneedlesÆ1–septaextractorÆ6–boxesofKimwipesÆReamofpaperÆ3–ExtrablackinkcartridgesÆ3–ExtracolorinkcartridgesÆboxof3.5inchdisksÆsetofinstructionsforsetupÆsetofinstructionsforrunningÆEffectsofMoleculesLabÆSepofAlcoholsLabÆGCofMouthwashLabNFPAInformation:ThetubrequiresanNFPAlabelof3,4,0. GCPrinterTubÆPrinterÆLaptopÆPowerStripÆMousepadÆUSB–SerialCableÆLaptopPowerCordÆMouseÆPrinterCableÆPrinterPowerCordÆ IRTubÆ2–boxesoflargeglovesÆ1–boxofmediumglovesÆ2–boxesofkimwipesÆ1–boxofPasteurpipettesÆ15-20–bulbsforPasteurpipettesÆ2–marblemortarandpestleÆ6-8–saltplates(25x4mmNaCl)Æ4–sampleholdersÆ1–reamofpaperÆ1–bottlemineraloilÆ1–1LbottleofacetoneÆ1–acetonewashbottleÆ1–wastebottleÆ1–9bottleslab(IRofliquids)Æ2–smallvialKBrÆ1–KBrpelletpressÆ2–4”or6”adjustablewrenchesÆ1–pelletpresssampleholderÆ1–funnelÆ2–rubberpolicemenÆ1–bagofo-ringsÆextraspectraÆplasticsamplesÆdirectionsÆ1–IRBookNFPAInformation:ThetubrequiresaNPFAlabelof3,4,2 IRPrinterTubÆPrinterÆLaptopÆPowerStripÆMousePadÆIRPowerCordÆDataCableÆLaptopPowerCordÆMouseÆPrinterCableÆPrinterPowerCordÆExtraBlackCartidgeÆExtraColorCartidgeÆFloppyDisksNOTE–BesurethatthecontentsofthetubarematchedupwithitsappropriateIR. SpecTubÆ4–boxesemptycuvettesÆ2–boxeschalkcuvettesÆ1–boxwaterblanksÆ2–boxesmaxwavelengthcuvettesÆ4–woodentesttubeholdersÆcrepepaperÆ2–boxesfoodcoloringÆparafilmÆ3–boxeskimwipesÆextrabulbsÆextrachalkÆcoloredwaterÆpipetsÆEducationalAbsorptionSpectraKit LiquidNitrogenTubÆ2–insulatedmugsÆ3-4–cansofracquetballsÆ1–bagofsmallballoonsÆ1–bagof12”clearballoonsÆ1–woodblockÆ10–nailsÆ1–superconductorkitÆ3–largePyrextesttubesÆ1–rubberhosewithglasstubeÆ1–small,thinmetalpanÆ2–boxesofmatchesÆ2–grilllightersÆ2–insulatedglovesÆ1–crucibletongsÆ3-4–6”piecesoftygontubingÆ5-6–StyrofoamcupsÆHammerÆwrenchÆmethaneregulatorÆleadbellOthernecessities:ÆglassdewarÆliquidnitrogenOtherSupplies:Dependingonthelengthofshoworifthetubisbeingdropped-off,thefollowingitemsmaynotbeneeded.Checkwiththemobileeducatortoconfirm.ÆbananasÆflowersÆmethanetankÆoxygentank NuclearTubÆ12–setsofsourcesÆ12–forcepsÆ12–shelvesÆ6–shieldingkitsÆ2–Cs/BaminigeneratorsÆ1–bagofmetalplanchetsÆ2–bottlesofeluatingsolutionÆ1–boxofmediumglovesÆ1–boxoflargeglovesÆVariousMetalShieldsÆ1–bagofnotecardsÆNotecardUnknownsÆVariousBoxesofPennies ItemstobekeptontheVanatAllTimesGlasswareo12–400mLBeakerso12–250mLBeakerso12–50mLBeakerso12–30mLBeakerso10–100mLVolumetricFlaskso10–50mLVolumetricFlaskso10–100mLGraduatedCylinderso10–50mLGraduatedCylinderso10–10mLGraduatedCylinderso12–50mLBuretso12–MicroscaleKitso1–PipetBoxAccessorieso24–PairsofSafetyGlasses(adult&elementary)o4–BoxesofKimwipeso1–ReamofPapero1–RollofFisherRecorderPapero1–RollofLinearRecorderPapero4–PowerStripso4–ExtensionCordso5-10–BungeeCordso1–Bundle/RollofPaperTowelso1–Carto12–BuretClampso12–LargeTestTubeRackso12–SmallTestTubeRackso1–BoxofHotPlates(8)o1–BoxofMicroscaleThermometersInstrumentationo6–Spec20’so6–GC’so8–Recorderso12–pHMeterso12–MelTempso4-6AnalyticalBalanceso4-6TopLoaderBalances TableofContentsforExperimentsPhysicalPropertiesDensityofaLiquidMicrodensityofPlasticsDeterminationofMeltingPointsDeterminationofBoilingPointsAccuracyandPrecisionConductivityofSolutionsBondTypesandPhysicalPropertiesBoyle’sLawCharles’LawMicroscaleTechniquesMicroanalysisofVitaminCinCitricJuicesEsterificationMicroscaleSynthesisofAcetylsalicylicAcidSimpleDistillationChromatographyChromatographyofMagicMarkers/RadialChromatographyCandyChromatographyTLCofFoodDyesTLCSeparationofAnthraquinonesTLCofVegetablesTLCofAnalgesicsExtractionandIdentificationofDyes(Prep-sepofKool-Aid)SeparationofMethyleneBlueandFluorosceinGasChromatographyEffectsofMoleculesonRetentionTimesSeparationofAlcoholsSpikingAnalysisofDryGasVerificationofEsterificationGCofMouthwashNuclearChemistryIsotopesofAtomsHalf-LifeSimulationOperatingVoltageStatisticsofCountingIntroductiontoRadiationHalf-LifeofBarium-137MElectrophoresisElectrophoresisofAminoAcidsChemicalReactionsOxidizingPowerofLaundryBleachHotandColdReactionsSpectrophotometryASpectrumDeterminationofWavelengthofMaximumAbsorbanceCrepePaperAnalysisAnalysisofSuntanLotionDeterminationofIroninWaterDeterminationofIroninFoodSpectrophotometricAnalysisofAspirinSpectrophotometricDeterminationofVariousMetalsDeterminingNOx’sinCarExhaustUVDeterminationofCaffeineContentPreparationofSolutionsSpectrophotometricDeterminationofpHSpectrophotometricDeterminationofCopperinaPennyBradfordProteinAssayInfraredSpectroscopySpectraofPureLiquidsSpectraofSolids/KBrPelletPressInfraredSpectroscopyofGasesTheTheoryofpHpHTitrationCurvepHTitrationofOxalicAcidAnalysisofDrainCleanersAceticAcidinVinegarABufferpHofHouseholds/39DropsIdentificationLabsIdentificationofanUnknownGasIdentificationofSolutionsMysterySolutionsBloodTestingChemicalEquilibriumUsingLeChatelier’sPrincipleKSPofCopper(II)TartrateKineticStudyoftheReactionofKIwithK2S2O8gPhysicalProperties: DensityofaLiquidgpipetboxg50mLbeakersgweighboatsgbalances(analyticaland/ortoploading)g4–125mLofeachofthefollowingliquids:gDe-ionizedwater(density=1g/mL)gMethanol(density=0.8127g/mL)gSaturatedNaI(density=1.918g/mL)gSaturatedNaCl(density=1.20g/mL)gLiquidsofunknowndensitiesgUnknown#1–sat.NaCl-density=1.918g/mLgUnknown#2-ethanol-density=0.7893g/mNFPAInformation:ChemicalHealthFireReactivitysodiumiodide200methanol130sodiumchloride200 PhysicalPropertiesMicrodensityofPlasticsg10-12-forcepsg10-12-stirringrodsg25-Borosilicatedisposableculturetubesg10-smalltesttuberacksg1-pipettebox(automaticpipettes,50mLbeakers)g50-100-smallweighboatsg8-thinstempipetteslabeledmethanolg8-thinstempipetteslabeledNaIg8-125mLbottlesofmethanolg8-125mLbottlesofSaturatedNaIganalyticalbalancesg1–orbitzdrinkgknownplasticsamples(cutinapproximately4x4mmsquares)g#1-PETE-polyethyleneterephthalate-density=1.39g#2-HDPE-highdensitypolyethylene-density=0.95-0.97g#3-PVC-polyvinylchloride-density=variesg#4-LDPE-lowdensitypolyethylene-density=0.92-0.94g#5-PP-polypropylene-density=0.90-0.91g#6-PS-polystyrene-density=1.05-1.07g#7-other(oftenamixture)-density=variesgunknownplasticsamples(cutinapproximately4x4mmsquares)gUnknownA-#5-PPgUnknownB-#2-HDPEgUnknownC-#1-PETEgUnknownD-#6-PSgUnknownE-#4-LDPE**Onlyplastic#1,#2,#4,#5,#6areusedbecauseplastics#3and#7densitiesvary.Preparationofsolutions:gSaturatedNaI–dissolve185gofNaIper100mLofwater(useabeaker,notavolumetricflask)NFPAInformation:ChemicalHealthFireReactivityMethanol130SatNaI200 PhysicalPropertiesDeterminationofMeltingPointsgavailableMel-TempsgDigitalThermometersg2–VialsofClosed-endCapillaryTubesg6–MortarandPestles(ifnecessary)g6–DroppingTubesg1–CapillaryTubeWasteBottleg6–vialsofthefollowingsolids:gbenzoinm.p.-137oCgethyl-p-aminobenzoatem.p.-88/90oCgpalmiticacidm.p.-63oCgp-nitrobenzaldehydem.p.-106/107oCgstearicacidm.p.-68/69.5oCgvanillinm.p.-81/83oCg2-setsofthefollowingunknowns:gUnknown#1-vanillingUnknown#2-p-nitrobenzaldehydegUnknown#3-ethyl-p-aminobenzoategUnknown#4-palmiticacidgUnknown#5-benzoingUnknown#6-stearicacidg2-setsofthefollowingmixtures:gMixtureA-ethyl-p-aminobenzoateandstearicacidgMixtureB-ethyl-p-aminobenzoateandbenzoingMixtureC-palmiticacidandp-nitrobenzaldehydegMixtureD-vanillinandbenzoingMixtureE-vanillinandstearicacidNFPAInformation:ChemicalHealthFireReactivityvanillin210palmiticacid110benzoin210ethyl-p-aminobenzoate111stericacid110p-nitrobenzaldehyde210 PhysicalPropertiesDeterminationofBoilingPointsg6-10-hotplatesg8-12MicroscaleThermometersg10-12-250mLbeakerg2-vialsofclosedendcapillarytubesg25-50-borosilicatedisposableculturetubesg8-ringstands(ifneeded)g16-adapterclamps(ifneeded)g16-three-prongedclamps(ifneeded)g8-testtubeholdersgDropperslabeledforeachoftheliquidandunknownsg8-60mLbottlesofthefollowingliquids:gacetone-b.p.56-57oCgmethanol-b.p.65oCgethanol-b.p.78-79oCgpropanol-b.p.97-98oCg2-propanol-b.p.82-83oCg2-60mLbottlesofunknownsgunknown#1-2-propanolgunknown#2-ethanolgunknown#3-methanolgunknown#4-acetonegunknown#5-1-propanolgWasteBottleNFPAInformation:ChemicalHealthFireReactivityacetone130ethanol030methanol1301-propanol1302-propanol130 PhysicalPropertiesAccuracyandPrecisiong1-pipettebox(pipettes,automaticpipettes,beakers)g8-thermometersg8-250mLbeakersg8-10mLgraduatedcylindersg8-50mLgraduatedcylindersg#ofavailable-toploadingbalancesg#ofavailable-analyticalbalancesg1-carboyofde-ionizedH2OgThefollowingchartisforthedensityofwateratvarioustemperatures:Temperature(°C)Density(g/cm3)00.99984100.99970200.99821300.99565400.99222500.98803600.98320700.97778800.97182900.965351000.95840 PhysicalPropertiesConductivityofSolutionsgavailableconductivityapparatig3-4-extra9Vbatteriesg12–24-holeWellPlatesg8-vialsofNaClg8-vialsofsucroseg16–spatulasg“BAD”BatteryLabelsgExtraCoversforMeterEndsg8-labeleddroppersofeachsolutiong8-30mLbottlesofeachofthefollowing:gglacialaceticacidgdistilledwaterg0.1MNaClg0.1Mpotassiumnitrateg0.1MHClg0.1Mammoniag0.1Msucroseg0.1Maceticacidg0.1MNaOHgethanolgmethanolPreparationofsolutions:g0.1MNaCl-dissolve2.925gofNaClin500mLofDE-IONIZEDwaterg0.1Mpotassiumnitrate-dissolve5.055gofpotassiumnitratein500mLofDE-IONIZEDwaterg0.1MHCl-dilute4.3mLofconc.HClin500mLofDE-IONIZEDwaterg0.1Mammonia-dilute3.4mLofconc.ammoniumhydroxidein500mLofDE-IONIZEDwater.g0.1Msucrose-dissolve17.11gofsucrosein500mLofDE-IONIZEDwaterg0.1Maceticacid-dilute2.9mLofglacialaceticacidin500mLofDE-IONIZEDwaterg0.1MNaOH-dissolve2gofNaOHin500mLofDE-IONIZEDwaterNFPAInformation:ChemicalHealthFireReactivityChemicalHealthFireReactivityaceticacid320sucrose110NaCl200NaOH300KNO3100ethanol030HCl300methanol130ammonia300 PhysicalPropertiesBondTypesandPhysicalPropertiesg12–24-holeWellPlatesg2–vialsofclosed-endcapillarytubesg6–droppingtubesgavailablemel-tempsgavailabledigitalthermometersg6–stirringrodsg6–washbottlesg6–20mLvialsofthefollowingsolids:gsodiumchloridegmagnesiumsulfategnaphthalenegacetylsalicylicacidg6–60mLbottlesofcookingoilg6–Scoopulasg50–SmallWeighBoatsNFPAInformation:ChemicalHealthFireReactivitysodiumchloride200magnesiumsulfate100naphthalene220acetylsalicylicacid210 PhysicalPropertiesBoyle’sLawg1-BagofSealed,FilledPipetsg12-RulersNFPAInformation:NoneneededCharles’Lawg1-BagofPipetsg12-HotPlatesg12-DigitalorMicroscaleThermometersg12-250mLbeakersNFPAInformation:Noneneeded MicroscaleTechniquesMicroanalysisofVitaminCinCitrusJuicesµ12-30mLbottleslabeledstarchsolutionµ12-30mLbottlesofiodinesolutionµ1-500mLbottleofiodinesolutionµ12–24-holeWellPlatesµ12-30mLbottleslabeledvitaminCsolutionµ12-dropperslabelediodineµ12-dropperslabeledstarchµ12-dropperslabeledvit.CµSampleDroppers:µ2–Sample#1µ2–Sample#2µetc…µ4-6-differenttypesoffruitjuicesµ1-coolerfilledwithiceµ1-500mLbottleofDE-IONIZEDwaterµ1-forcepsµ12-plasticcupsµ1-SharpieµVolumetricFlaskwithStarch**µVolumetricFlaskwithVitaminC****Note-ThevitaminCandstarchsolutionsmustbepreppedthesamedayasthelab.ThismeansthatavolumetricflaskcontainingtheproperamountofvitaminCmustbetapedshutandthevandriverwilladdthewaterattheschool.Thesizeoftheflasksentwilldependonthenumberofclassesandclasssizes.Preparationofsolutions:µVitaminC(1mg/mL)-Dissolve250mgofascorbicacidin250mLofDE-IONIZEDwater.µIodineSolution–Making1Lofthissolutionshouldlastyouallyearanditneedsrepreppedeverysummer.1.Dissolve1.2gI2in50mLofethanol2.Oncedissolvedtransfersolutionto1LVolumetricFlask3.Add1.2gKI4.DilutewithDe-IonizedwaterµStarchSolution–Dissolve2.5mLofLinitLaundryStarchin250mlde-ionizedH2O.NFPAInformation:ChemicalHealthFireReactivityStarch110Iodine231VitaminC110 MicroscaleTechniquesMicroscaleProductionofHydrogenµ12–20mLVialsofMgPowderµ12–30mLBottlesofDishSoapµ12–Spatulasµ12–60mLBottlesof1.0MHClµ12–MedicineCupsµ12–PiecesofRubberTubingµ1–bagoflargeballoonsµ12–RubberStoppers(withaholetoholdthecandles)µ12–PlasticBowlsµ30-40–SmallTestTubesµ12–BirthdayCandlesµ2–PacksofMatchesµ12–60mLSyringesµ50–SmallWeighBoatsµ24–RubberBandsµ24–SyringeCapsµ24–PlasticVialLidsµ12–AdapterClamps(ifnecessary)µ12–LargeTestTubeClamps(ifnecessary)µ12–RingStands(ifnecessary)µavailableAnalyticalBalancesµ8–DIWaterBottlesPreparationofSolutions:µ1.0MHCl-Dilute43mLofconc.HClto500mLwithDE-IONIZEDwaterµ3%DishSoap–Dilute30mLofanyDishSoapto1LNFPAInformation:ChemicalHealthFireReactivityHCl300MgPowder333 MicroscaleTechniquesEsterificationµ8-hotplatesµ8–400or600mLbeakersµ25-Styrofoamcupsµ8–60mLbottlesofglacialaceticacidµ8-vialsofsalicylicacidµ8–60mLbottlesofisoamylalcoholµ8–125mLbottlesofmethanolµ8–125mLbottlesofethanolµ8-vialsofDowex50x2-100ionexchangeresinµ8–MicroscaleKitsµ2–rollsoflabelingtapeµ2–sharpiesµ8–dropperslabeledMethanolµ8–dropperslabeledEthanolµ8–dropperslabeledGlacialAceticAcidµ8–dropperslabeledIsoamylAlcoholµ8–MicroscalethermometersNFPAInformation:ChemicalHealthFireReactivityMethanol130Ethanol030GlacialAceticAcid320IsoamylAlcohol120Dowex50x2–100ionexchangeresin010SalicylicAcid010 MicroscaleTechniquesMicroscaleSynthesisofAcetylsalicylicAcidµ8-vialsofsalicylicacidµ8-30mLbottlesofaceticanhydrideµ8-30mLbottlesofconcentratedphosphoricacidµ8ormore-microscalekitsµ8-microscalethermometersµ2-boxesof11.0cmfilterpaperµ50-smallweighboatsµ1-analyticalbalanceµ8-hotplatesµ1-cooleroficeµ1bagofpipetsµ8-250mLbeakerµ8-StyrofoamcupsµavailablehandpumpsNFPAInformation:ChemicalHealthFireReactivitySalicylicAcid010PhosphoricAcid300AceticAnyhydride321 MicroscaleTechniquesSimpleDistillationµ8ormore-microscalekitsµ8--10°C-150°Cthermometersµ3-vialsofboilingchipsµ8-sandbathsµ8-hotplatesµ16-adapterclampsµ16-3-prongclampsµ8-100mLbeakerµ8-ringstandsµ1-containeroficeµ4-125mLbottlesofeachofthefollowingorganicliquidsµAcetoneb.p.56-57oCµMethanolb.p.65oCµEthanolb.p.78-79oCµn-propanolb.p.97-98oCµ2-propanolb.p.82-83oC Chromatography:ChromatographyofMagicMarkersÓ35-50-18x150mmorlargertesttubesÓ10ormore-testtuberacksÓ15ormore-watersolubleblackmagicmarkersÓ5-washbottles(water)Ó100ormore-precutchromatographystripsRadialChromatographyÓ35-50-smallpetridishesÓ30ormore-watersolublecoloredmagicmarkersÓ5-washbottles(water)Ó2-boxesof7.0cmfilterpaperÓ2-boxesof11.0cmfilterpaperwithaholeinthecenter(holeisoptional)NFPAInformation:NoneRequired ChromatographyCandyChromatographyÓ12–250mLbeakersÓ6–125mLbottlesofvinegarÓ2-vialsofspottingtubesÓ8-pencilsÓ8-rulersÓ50ormore-stripsofchromatographypaper(4x20cm)Ó1-rollplasticwrapÓ8–3mLvialsofconcentratedM&McandycoatingdyeÓ8–3mLvialsofconcentratedSkittlescandycoatingdyeÓWastebottlesforspottingtubesÓ2-3mLvialsoffollowingfooddyes:ÓBlue#1ÓBlue#2ÓRed#3ÓRed#40ÓYellow#5ÓYellow#6***NOTE***-BlueDyesneedtobereprepedeverytimeiftheyarenotblueanymore.Preparationofsolutions:ÓCandydyes-1.Placecandyinabeakerwithenoughvinegartocoverthetopofthecandy.(useallcolorsandextragreen)2.Heatgentlyuntilcolorcoatingdissolves.3.Pourdyesolutionintoabeakerandaddapieceofwoolclothandaddalittlemorevinegar.4.Boilgentlyforafewminuteswhileconstantlystirring.5.Rinsethewoolwithwater.6.Inacleanbeaker,placethewoolandsomehouseholdammonia.7.Testthesolutionwithlitmuspapertomakesureitisbasic.8.Concentratethesolutionbyboilingitto¼itsoriginalvolume.ÓStandardDyes–addonemicrospatulaofdrydyeto125mLofwater.NFPAInformation:ChemicalHealthFireReactivityDyes(outsideofcontainers)310 ChromatographyThinLayerChromatographyofVegetablesÓ2-boxesof2.5x7.5cmBakerflexsilica-gelIB-FTLCslidesÓ24-developingchambersÓ2-vialsofspottubesÓ8-12-mortarsandpestlesÓ50-thinstemmedpipettesÓ25-borosilicatedisposableculturetubesÓ8-smalltesttuberackÓ1-bagofspinachÓ1-containerofshreddedcarrotsfromagrocerystoresaladbarÓ8-125mLbottlesofhexaneÓ8-125mLbottlesofacetoneÓ8-125mLbottlesof70:30hexane/acetonedevelopingsolutionÓ1-wastebottle(hexane,acetone,andveggiewaste)Ó8-funnelsÓ1-packof15cmfilterpaperÓ6–flashlightsÓ8–pencilsÓ8-rulersPreparationofsolutions:Ó70:30hexane/acetonedevelopingsolution-add300mLacetoneto700mLofhexaneNFPAInfromations:ChemicalHealthFireReactivityHexane130Acetone23070:30Hexane:Acetone(developingsolution)230 ChromatographyTLCofAnalgesicsÓ2-vialsofspottubesÓ8-developingchambersÓ8–mortarsandpestelsÓ8–50mLbeakersÓ2-UVlightsÓ8-rulersÓ8–pencilsÓ1–boxofPasteurPipetsÓ1–ballofcottonÓ1–1Lwastebottle(toluene,ethylformate,formicacid,ibuprofen,acetylsalicylicacid,acetaminophen,caffeine,naproxen,over-the-counterpainrelievers)Ó1–bottleoftheavailableover-the-counterpainrelieversÓ2-boxesof2.5x7.5cmBakerflexsilica-gelIB-FTLCslidesÓ1–500mLbottleof16:4:1toluene:ethylformate:88%formicacidÓ1–500mLbottleof19:1ethylformate:88%formicacidÓ8–60mLbottlesof16:4:1toluene:ethylformate:88%formicacidÓ8–60mLbottlesof19:1ethylformate:88%formicacidÓ3–30mLbottlesofthefollowingknowns:ÓibuprofenÓacetylsalicylicacidÓacetaminophenÓcaffeineÓnaproxenÓ3–30mLbottlesofthefollowingunknowns:ÓExcederinÓBayerÓTylenolÓAlleveÓAdvilÓanyotherover-the-counterpainrelieverPreparationofsolutions:Ó16:4:1toluene:ethylformate:88%formicacid(developingsolution)–Ina1Lgraduatedcylinder,measure800mLoftoluene.Filltothe1Lmarkwithethylformate(inotherwords,add200mLofethylformate).Add50mLof88%formicacidtothegraduatedcylinder(thereshouldbeenoughheadspaceinthecylindertoaccommodate50mL).Ó19:1ethylformate:88%formicacid–Ina1Lgraducatedcylinder,measure950mLofethylformate.Filltothe1Lmarkwith88%formicacid(inotherwords,add50mLofformicacid).ÓKnownSolutions–Dissolve1gofthepuresolidin100mLofthe19:1ethylformate:88%formicacidsolution.ÓUnknownSolutions–1.Crushtwoofthepainrelievertabletswithamortarandpestel. 1.Usingaburetorchromatographycolumn,packasmallamountofglasswoolatthebottomofthecolumn(beforethestopcock).2.Carefullyscoopthecrushedanalgesicintothecolumn.3.Placea125mLErlenmeyerflaskatthebottomofeachcolumn.4.Measure100mLofthe19:1ethylformate:88%formicacidsolutionforeachofthecolumns.5.Addthesolutiontothecolumnandcovertheremainingsolutionwithparafilm.6.Openthestopcockoneachofthecolumnsverycarefully!Youdon’twantanyofthesolidinthesolution.Ifyoudogetsolidinthesolution,letitsettleovernightanddecanttheliquid.7.Fillthecolumnsasneededuntilyouhaveusedthetotal100mLof19:1ethylformate:88%formicacidsolution.NFPAInformation:ChemicalHealthFireReactivity16:4:1toluene:ethylformate:88%formicacid33019:1ethylformate:88%formicacid330KnownSolutions330UnknownSolutions330 ChromatographyPrep-SepofKool-aidÓ12–24-holeWellPlatesÓ50-prepsepC-18extractioncolumn(Fisher#P453)Ó30-5mLsyringesÓ30-Bio-Rad3-waylureÓ12-vialsofgrapeKool-aidÓ1–500mLbottleofnon-grapeKool-aidÓ7-30mLbottlesofthefollowingisopropanol/watersolutionsÓ100%isopropanolÓ20%isopropanolÓ10%isopropanolÓ5%isopropanolÓDe-ionizedwaterÓ1-250mLofeachoftheaboveisopropanol/watersolutionsPreparationofsolutions:Ó20%isopropanol-add200mLofpureisopropanolto800mLwaterÓ10%isopropanol-add100mLofpureisopropanolto900mLwaterÓ5%isopropanol-add50mLofpureisopropanolto950mLwaterÓKool-AidSolutions-add1packofKool-aidto2LwaterNFPAInformation:ChemicalHealthFireReactivityIsopropanolSolutions130 GasChromatography:EffectsofMoleculesonRetentionTimesPavailableGCsPavailabelaptop/printertubsP6-boxesofkimwipes(GCtub)PGCSyringes(GCtub)PSamplevialswithdifferentvolatileliquids:(GCtub)P6-methanolP6-ethanolP6-n-propanolP6-n-butanolP6-n-pentanol(amylalcohol)P6-n-hexanolP6-n-heptanolP6-2-butanolP6-iso-butanolP6-tert-butanolP6-pentaneP6-hexaneP6-heptane**Note:pentane,hexane,andheptanewillevaporateveryquicklysothesemustbecheckedeverytimethelabgoesoutNFPAInformation:ChemicalHealthFireReactivityBoxofvialsforthislab240 GasChromatographySeparationofAlcoholsPavailableGCsPavailablelaptop/printertubsP6-boxesofkimwipes(GCtub)PGCSyringes(GCtub)P6-vialsoftheknownalcohols(GCtub)P1-butanolP2-butanolP2-methyl-1-propanolP3-5-vialsoftheunknownalcoholmixturesPreparationofSolutions:Unknown#1-butanol2-butanol2-methyl-1-propanol150%------------------50%233.3%33.3%33.3%3------------------30%70%440%60%---------------------520%80%---------------------NFPAInformation:ChemicalHealthFireReactivityBoxofvialsforthislab230 GasChromatographySpikingAnalysisofDryGasPavailableGCsPavailablelaptop/printertubsPGCSyringes(GCtub)P25-40–20mLvialsP3-5-gasolineadditivesP50-micropipettesP1-rolloflabelingtapeP4-125mLbottlesofeachofthefollowingorganicliquidsPethanolPmethanolPacetoneP2-propanolNFPAInformation:ChemicalHealthFireReactivityEthanol030Methanol130Acetone2302-propanol130 GasChromatographyVerificationofEsterificationP6-60mLbottlesofglacialaceticacidP6-60mLbottlesofethanolP6-60mLbottlesofisoamylalcoholP6-vialsofdowex50x2-100ion-exchangeresinP6-5mLvialsofethylacetateP6-5mLvialsofisoamylacetatePavailableGCsPavailablelaptop/printertubsPGCSyringes(GCtub)NFPAInformation:ChemicalHealthFireReactivityGlacialAceticAcid320Ethanol030IsoamylAlcohol120Doxex50x2–100ion-exchangeresin010EthylAcetate130IsoamylAcetate130 GasChromatographyGCofMouthwashPavailableGCsPavailablelaptop/printertubsPGCSyringes(GCtub)P6-20mLvialsofdifferentoverthecountermouthwashesP6-GCvialsofeachofthefollowingethanolsolutionsPSolutionA-5%ethanolPSolutionB-10%ethanolPSolutionC-20%ethanolPSolutionD-30%ethanolPreparationofSolutions:PMouthwashsolutions-pourthemouthwashdirectlyfromthebottleinto20mLvialsPEthanolsolutions-P5%-5.26mLof95%ethanoldilutedto100mLwithwaterP10%-10.53mLof95%ethanoldilutedto100mLwithwaterP20%-21.05mLof95%ethanoldilutedto100mLwithwaterP30%-31.58mLof95%ethanoldilutedto100mLwithwaterNFPAInformation:ChemicalHealthFireReactivityEthanol030 Electrophoresis:ElectrophoresisofAminoAcids£availableelectrophoresisunits£1-boxcellulosechromatogramsheets£10–electrophoresisplates,cutto5cmx15cm£2-vialsofspottubes£8-100mLgraduatedcylinder£4ormore-hotplates£8-rulers£8-boxesofkimwipes£Pasteurpipetsandbulbs£pencils£1–boxmediumgloves£1–boxlargegloves£1–500mLspraybottleofninhydrinspray£1-2LbottleofphosphatebufferpH=6£1–60mLbottleofeachofthefollowingaminoacidsolutions:£0.125Marginine(maybereplacedbylysine)£0.125Mcysteine£0.125Mglutamicacid(maybereplacedbyasparticacid)£0.125Mglycine(maybereplacedbyalanine,phenylalanine,serine,orvaline)£0.125Mhistidine£1–30mLbottleofthefollowingunknownaminoacidsolutions:£UnknownA:glutamicacid,glycine,histidine£UnknownB:argenine,cysteine,glutamicacid£UnknownC:argenine,glutamicacid,glycine£UnknownD:cysteine,glutamicacid,histidinePreparationofsolutions:£PhosphatebufferpH=61.Dissolve6.80gpotassiumdihydrogenphosphate(potassiumphosphatemonobasic)in500mLofDIwater.2.Transferthesolutiontoa1Lvolumetricflask.3.Add56mLof0.1MNaOHtotheflask.4.Diluteto1L.CheckthatthepH=6andadjustifnecessary.£NinhydrinSpray-Dissolve0.75gofninhydrinin250mLofn-butanol.Thenadd7.5mLofglacialaceticacid.Thiswilllastfor2-3weeks.Ifunsurethatitwillwork,testthespraybeforeuse. £StandardAminoAcidSolutions–CHECKYOURAMINOACIDS!!Aminoacidscomeinvariousforms(hydrochloridevs.not,etc.),sothemolecularweightsaregivenhereasaneasycheck.Ifthemolecularweightsdiffer,youwillneedtorecalculatetheamountofsolidneeded£Arginine,MW=210.7g/mol£Dissolve1.32gofthesolidin50mLofDIwater.£Glutamicacid,MW=147.13g/mol£Dissolve0.920gofthesolidin50mLofDIwater.£Glycine,MW=75.06g/mol£Dissolve.469gofthesolidin50mLofDIwater.£Histidine,MW=191.7g/mol£Dissolve1.20gofthesolidin50mLofDIwater£Cysteine,MW121.15g/mol£Dissolve.757gofthesolidin50mLofDIwater.£UnknownAminoAcidSolutions–Mix5mLofeachofthestandardsolutions.NFPAInformation:ChemicalHealthFireReactivityarginine000glutamicacid210glycine210histidine110cysteine100phosphatebuffer300ninhydrinspray330 eChemicalReactionsOxidizingPowerofLaundryBleache8–Buretse8–Buretclampse8–Magneticstirrerse8–250mLErlenmeyerflaskse8–250mLvolumetricflaskse8-scoopulasepipetboxe8–washbottlese8–50mLbeakerseavailableanalyticalbalancese8-10mLgraduatedcylinderse1-2Lbottlesof0.100Msodiumthiosulfatee8-100mLgraduatedcylinderse8-vialsofpotassiumiodideewastecontainerestarchsolutione8-60mLvialsofglacialaceticacide2-3differentbrandsofbleachor2LoreachbleachsolutionPreparationofsolutions:ebleachsolution–40mLbleachdilutedto2Le49.634gofNa2S2O3*5H2Odilutedto2L.Thissolutionshouldbepreparedfresh.eStarchsolution–spraysmallamountofstarchintobeakerofdistilledwater.Thevandrivercandothis–labassistantssendaspraybottleofstarchandabeaker. ChemicalReactionsHotandColdReactionse6vialsofthefollowingchemicals:ecalciumchlorideeammoniumchlorideebariumhydroxideelabeledscoopulase4-6washbottlese20papercupse8–50mLgraduatedcylindersewoodsplintseWasteContainerforbariumhydroxideNFPAInformation:ChemicalHealthFireReactivityammoniumchloride200calciumchloride201bariumhydroxide200 NuclearChemistryIsotopesofAtomsα20-pre1982penniesα20-post1982penniesα2-4-toploadingbalancesα10-filmcanisterswith10penniesinsidePreparationofcanisters:αFilmcanisters-recordthemassofthe10filmcanistersandlidsbeforeanypenniesareplacedinside. NuclearChemistryHalf-LifeSimulationα25-50(dependingonthenumberofstudentsintheclass)-plasticbagswith100M&M’sα25-50-clean,unusedpaperplates NuclearChemistryOperatingVoltageα6-8-scalarswithGMtubeα6-8-betasources(NuclearTub)α6-8–forceps(NuclearTub)α6-8–shelves(NuclearTub) NuclearChemistryStatisticsofCountingα6-8-scalarswithGMtubeα6-8-betasources(NuclearTub)α6-8–forceps(NuclearTub)α6-8–shelves(NuclearTub) NuclearChemistryIntroductiontoRadiationα6-8-scalarswithGMtubeα6-8-alphasources(NuclearTub)β6-8-betasources(NuclearTub)γ6-8-gammasources(NuclearTub)α6-8–forceps(NuclearTub)α6-8–shelves(NuclearTub)α6-setsofshields(NuclearTub) NuclearChemistryHalf-LifeofBarium-137mα6-8-scalarswithGMtubeα2-Cs/Baminigenerator(NuclearTub)α15–planchet(NuclearTub)α6-8–shelvesα2–bottlesofelutingsolution(NuclearTub)α1–boxofmediumgloves(NuclearTub)α1–boxoflargegloves(NuclearTub)α6-8–forceps(NuclearTub) SpectrophotometryASpectrumLavailablespectrophotometersL1-boxofchalkcuvettes(SpecTub)Preparation:LChalkcuvettes-placeonepieceofwhitechalk2-3cmlonginthecuvette.Thechalkmusthavea45°angleforaface.NFPAInformation:Noneneeded. SpectrophotometryDeterminationofWavelengthofMaximumAbsorbanceLavailablespectrophotometersL1–boxDIH2Oblanks(SpecTub)L2-cuvettesofeachcolorofwater(12total)(SpecTub)LredLorangeLyellowLgreenLblueLpurplePreparationofsolutions:LBluewater-addbluefoodcoloringto500mLofwater.LRedwater-addredfoodcoloringto500mLofwater.LYellowwater-addyellowfoodcoloringto500mLofwater.LGreenwater-addgreenfoodcoloringto500mLofwater.LOrangewater-addyellowandredfoodcoloringto500mLofwater.LPurplewater-addredandbluefoodcoloringto500mLofwater.NFPAInformation:Noneneeded. SpectrophotometryCrepePaperAnalysisLavailablespectrophotometersLassortedcolorsofcrepepaper(SpecTub)L8–50mLbeakersL8–10mLgraduatedcylinderL8-stirringrodL1–boxemptycuvettes(SpecTub)L1–boxwaterblanks(SpecTub)L8-hotplatesNFPAInformation:Noneneeded. SpectrophotometryAnalysisofSuntanLotionsLavailablespectrophotometersL2–boxesemptycuvettes(SpecTub)L4–125bmLbottlesofn-propanolLWasteBottle(n-propanolandsuntansolutions)L1–bagjumbopipets(SpecTub)L1–500mLbottleofthefollowingsuntanlotions:LSPF8LSPF15LSPF30LHaveatleastoneofeachandthendifferentbrandsofthesameSPFPreparationofsolutions:LSuntansolutions-Dissolve1gofsuntanlotionin500mLofn-propanol.NFPAInformation:ChemicalHealthFireReactivityn-propanol130suntansolutions130 SpectrophotometryDeterminationofIroninWaterLavailablespectrophotometersL8–125mLbottlesof100ppmFe2+L8–125mL0.25%ortho-phenanthrolineL8–DIWaterWashBottlesL1-pipetteboxL36–EmptyCuvettes(SpecTub)L8–100mLvolumetricflasksLfilledcuvettesofthefollowingIronSolutions:L2.0ppmFe2+L4.0ppmFe2+L6.0ppmFe2+L8.0ppmFe2+LUnknownC(5.0ppmFe2+)LUnknownC(3.0ppmFe2+)Preparationofsolutions:L100ppmFe2+-dissolve0.7022gofferrousammoniumsulfate,hexahydrateinwateranddiluteto1L.L0.25%ortho-phenanthroline-dissolve2.5gortho-phenanthrolinein50mLofethanol.Dilutethissolutionto1LwithDE-IONIZEDwater.LIronsolutions:L2.0ppm-place2.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L4.0ppm-place4.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L6.0ppm-place6.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L8.0ppm-place8.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.NFPAInformation:ChemicalHealthFireReactivityFe2+Solution200o-phenanthroline210IronSolutions210 LSpectrophotometrySpectrophotometricDeterminationofAspirinL12-ErlenmeyerflasksL6–10mLgraduatedcylinderL6–250mLvolumetricflaskL6–50mLvolumetricflaskL1-pipetteboxL36-cuvettes(SpecTub)LvariouscommercialaspirinproductsL6–20mLvialsofacetylsalicylicacidL6–125mLbottlesof1MNaOHL4L-0.02Miron(III)bufferL6-hotplatesLavaliablespectrophotometersL1–250mLbottleofaspirinstandardL24-cuvettes(SpecTub)L2–setsofthefollowingaspirinstandardsincuvettes:L16ppmAspirinL32ppmAspirinL48ppmAspirinL64ppmAspirinL80ppmAspirinL1–125mLbottleoftheabovestandardsPreparationofsolutions:L1MNaOH-Dissolve40gofNaOHin1Lofwater.L0.02MIron(III)Buffer–Prepare2Lof0.1MHClbydiluting8.6mLofconcentratedacid.Dissolve6.48gofFeCl3inthe2Lof0.1MHCl.LAspirinstandardsolution-1.Place400mgofacetylsalicylicacidina125mLErlenmeyerflask.2.Add10mLof1.0MNaOHandheatuntilboiling.3.Transferthesolutiontoa250mLvolumetricflaskanddilutewithwater. LAspirinstandardsL80ppm-Dilute5.0mLoftheaspirinstandardto100mLwithFe(III)buffer.L64ppm-Dilute4.0mLoftheaspirinstandardto100mLwithFe(III)buffer.L48ppm-Dilute3.0mLoftheaspirinstandardto100mLwithFe(III)buffer.L32ppm-Dilute2.0mLoftheaspirinstandardto100mLwithFe(III)buffer.L16ppm-Dilute1.0mLoftheaspirinstandardto100mLwithFe(III)buffer.NFPAInformation:ChemicalHealthFireReactivityNaOH300IronBuffer302AspirinStandardSolution310Aspirinstandards312 SpectrophotometrySpectrophotometricDeterminationofVariousMetalsLavailablespectrophotometersLBottlesofstandardsandunknownsofthefollowingmetals:L7–125mLBottlesofCu2+SolutionsL6–125mLBottlesofFe2+SolutionsL7–125mLBottlesofNi2+SolutionsL7–125mLBottlesofCo2+SolutionsL1boxoffilledcuvetsforeachmetalLSpecTubLWasteContainerforNi2+andCo2+Preparationofsolutions:LCu2+solutionsL0.10M–2.50gofCuSO4dissolvedin100mLofde-ionizedwater.L0.20M–5.00gofCuSO4dissolvedin100mLofde-ionizedwater.L0.30M–7.49gofCuSO4dissolvedin100mLofde-ionizedwater.L0.40M–9.99gofCuSO4dissolvedin100mLofde-ionizedwater.L0.50M–12.48gofCuSO4dissolvedin100mLofde-ionizedwater.LUnknownA(0.25M)–6.24gofCuSO4dissolvedin100mLofde-ionizedwater.LUnknownB(0.35M)-8.74gofCuSO4dissolvedin100mLofde-ionizedwater.LFe2+solutions100ppmFe2+and0.25%ortho-phenanthrolineareneededtopreparetheppmFe2+solutions.L100ppmFe2+-dissolve0.7022gofferrousammoniumsulfate,hexahydrateinwateranddiluteto1L.L0.25%ortho-phenanthroline-dissolve2.5gortho-phenanthrolinein50mLofethanol.Dilutethissolutionto1LwithDE-IONIZEDwater.**Thefollowingsolutionsneedtoberemadeeveryyear.**L2.0ppm-place2.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L4.0ppm-place4.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L6.0ppm-place6.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.L8.0ppm-place8.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater. LUnknownC(5.0ppm)-place5.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.LUnknownD(3.0ppm)–place3.0mLof100ppmstandardina100mLvolumetricflask.Add5mLof0.25%ortho-phenanthrolineanddiluteto100mLwithDIwater.LNi2+solutionsL0.10M–Dissolve2.63gofNiSO4in100mLofde-ionizedwater.L0.20M–Dissolve5.26gofNiSO4in100mLofde-ionizedwater.L0.30M–Dissolve7.89gofNiSO4in100mLofde-ionizedwater.L0.40M–Dissolve10.51gofNiSO4in100mLofde-ionizedwater.L0.50M–Dissolve13.14gofNiSO4in100mLofde-ionizedwater.LUnknownE(0.35M)–Dissolve9.21gofNiSO4in100mLofde-ionizedwater.LUnknownF(0.15M)-Dissolve3.29gofNiSO4in100mLofde-ionizedwater.LCo2+solutionsL0.10M–Dissolve2.91gofCo(NO3)2in100mLofde-ionizedwater.L0.20M–Dissolve5.82gofCo(NO3)2in100mLofde-ionizedwater.L0.30M–Dissolve8.73gofCo(NO3)2in100mLofde-ionizedwater.L0.40M–Dissolve11.64gofCo(NO3)2in100mLofde-ionizedwater.L0.50M–Dissolve14.55gofCo(NO3)2in100mLofde-ionizedwater.LUnknownG(0.25M)–Dissolve7.28ginCo(NO3)2to100mLofde-ionizedwater.LUnknownH(0.35M)-Dissolve10.19ginCo(NO3)2to100mLofde-ionizedwater.NFPAInformation:ChemicalHealthFireReactivityCu2+Solutions200Fe2+Solutions210Ni2+Solutions300Co2+Solutions200 SpectrophotometryDeterminationofNox’sinCarExhaustLavailablespectrophotometersLavailablebicyclepumpsL24–125mLErlenmeyerflasksL2-3-piecesofrubbertubingL2–boxesofcuvettes(SpecTub)L1-pipetteboxL1–WasteBottle(sulfanilamideandsodiumnitrite)L12–25mLvolumetricflasksL1-3-4LNalgenebeakerL1–1LgraduatedcylinderL4-2holestopperwith2smallPasteurpipettesthroughtheholeL1–2Lbottleof0.1MNaOHL2–500mLbottlesofsulfanilamidereagentL1–250mLbottleofN-NEDL1–250mLbottleof0.05%HydrogenperoxideL2–500mLbottlesofNaNO2ReagentPreparationofsolutions:L0.1MNaOH-dissolve4.0gofNaOHin1LofwaterLSulfanilamidereagent-1.Add300mLofwatertoa1Lvolumetricflask.2.Add20gofsulfanilamidetothewater3.Slowlyadd50mLofconc.phosphoricacid.Diluteto1L.LN-NEDreagent-N-(1-napthyl)-ethylenediaminedihydrogenchloride-place0.25gina250mLflask.Dilutewithwater.L0.05%Hydrogenperoxide-Dilute0.2mLof30%hydrogenperoxideto250mLwithwater.LNaNO2reagent-Dissolve0.06gofNaNO2in1Lofwater,anddilute30mLofthatsolutionto1LNFPAInformation:ChemicalHealthFireReactivityChemicalHealthFireReactivitysodiumhydroxide300hydrogenperoxide301sulfanilamidereagent310NaNO2reagent201 N-NEDreagent210 UVSpectrophotometryUVDeterminationofCaffeineContentLavailableUVspectrophotometersL6–250mLbottlesof1000ppmcaffeinestandardLavailablequartzcuvettes(UVTub)L6–125mLsepratoryfunnelsL6–SepratoryfunnelstoppersL1-pipetteboxLCansofvarioussodasL10–50mlvolumetricflasksL10–125mLErlenmeyerflasksL10–StoppersforErlenmeyerflasksL10–50mLgraduatedcylindersL4–500mLbottlesofmethylenechlorideL1–1Lbottleof1000ppmcaffeinestandardL1–bagjumbopipets(UVTub)L6–RingStands(ifnecessary)L6–ironrings(ifnecessary)L1–WasteBottle(methylenechloride)L6–250mLBeakersL6–MagneticStirrersL6–boxesofKimwipes(UVTub)Preparationofsolutions:L1000ppmCaffeinestandard-place1.000gofcaffeineina1Lflask.Dilutewithwater.NFPAInformation:ChemicalHealthFireReactivitymethylenechloride210caffeine210 UVSpectrophotometryUVAnalysisofSuntanLotionsLavailableUVSpectrophotometersLavailable–QuartzCuvettes(UVTub)L1–boxUV-gradePlasticCuvettes(UVTub)L6-125mLbottlesofn-propanolL1–bagjumbopipets(UVTub)L6–dropperslabeled“n-propanol”L2–extrarollsUVSpectrophotometerPaper(UVTub)L1–WasteContainer(n-propanolandsuntansolutions)L3–60mLbottleofthefollowingSuntanSolutions:LSPF8LSPF15LSPF30LHaveatleastoneofeachandthendifferentbrandsofthesameSPFPreparationofsolutions:LSuntanSolutions–Dissolve1gofsuntanlotionin500mLofn-propanol(SpectrophotometricAnalysisofSuntanLotions).Dilute15mLofthatsolutionin200mLofn-propanol.NFPAInformation:ChemicalHealthFireReactivityn-propanol130suntansolutions130 UVSpectrophotometryUVTubLavailablequartzcuvetsL1–boxofUVgradeplasticcuvetsL1–bagofjumbopipetsL2–rollsofextraspecpaper(onerollforeachtypeofUVspec)L6–boxesofkimwipesL1–bagofcutparafilm SpectrophotometryPreparationofSolutionsL10–50mLvolumetricflasksL10–25mLvolumetricflasksL10–100mLbeakersL10–250mLbeakersL10-magneticstirrersL50-storagevialsLavailableanalyticalbalancesL4-boxesofemptycuvets(SpecTub)LavailablespectrophotometersL1–WasteBottle(coppersulfateandnickelsulfate)L10–50mLgraduatedcylindersL10-droppersL1-pipetteboxL3–50gbottlesofcopper(II)sulfatepentahydrateL3–50gbottlesofnickelsulfatehexahydrateL2-setsofcopper(II)standardsincuvetsLconcentration#1-0.1000MLconcentration#2-0.0500MLconcentration#3-0.0100MLconcentration#4-0.0050ML2-setsofnickelsulfatestandardsincuvetsLconcentration#1-0.1000MLconcentration#2-0.0500MLconcentration#3-0.0100MLconcentration#4-0.0050MPreparationofsolutions:LCopper(II)Standards-L0.1000M-dissolve2.4968gofcopper(II)sulfatepentahydratein100mLofwaterL0.0500M-dilute50mLofthe0.1000Msolutionto100mLL0.0100M-dilute10mLofthe0.1000Msolutionto100mLL0.0050M-dilute50mLofthe0.0100Msolutionto100mL LNickelStandards-L0.1000M-dissolve2.6286gofnickelsulfatehexahydratein100mLofwaterL0.0500M-dilute50mLofthe0.1000Msolutionto100mLL0.0100M-dilute10mLofthe0.1000Msolutionto100mLL0.0050M-dilute50mLofthe0.0100Msolutionto100mLNFPAInformation:ChemicalHealthFireReactivitynickelsulfate300coppersulfate200 InfraredSpectrophotometrySpectraofPureLiquidseavailableIRseIRlaptops(IRPrintertub)eIRprinters(IRPrintertub)e1-9bottleslab(IRtub)e#1-hexanee#2-o-xylenee#3-dipropylaminee#4-toluenee#5-2-propanole#6-dimethylformamidee#7-ethylacetatee#8-2-butanone(MEK)e#9-heptaneNFPAInformation:ChemicalHealthFireReactivityBottle#1–Hexane130Bottle#2–o-Xylene230Bottle#3–Dipropylanime330Bottle#4–Toluene230Bottle#5–2-propanol142Bottle#6–DimethylFormate220Bottle#7–EthylAcetate130Bottle#8–2-butanone(MEK)242Bottle#9–Heptane130 InfraredSpectrophotometrySpectraofSolids/InfraredSpectraofSolidsUsingTheKBrpresseavailableIRseIRlaptops(IRPrintertub)eIRprinters(IRPrintertub)e2-vialsofKBr-infraredquality(IRTub)e2-vialsofeachofthefollowing:(IRTub)ebenzoicacidecitricacidetalc(babypowder)eacetylsalicylicacidesalicylicacidesodiumbicarbonate(bakingsoda)NFPAInformation:ChemicalHealthFireReactivityBenzoicAcid210CitricAcid210AcetylsalicylicAcid213SalicylicAcid010SodiumBicarbonate(bakingsoda)100 InfraredSpectrophotometryInfraredSpectroscopyofGaseseavailableIRseIRlaptops(IRPrintertub)eIRprinters(IRPrintertub)e2-3-packsofballoons(7”-9”)e1-dryiceorCompressedCO2e2-meterstickse1-airsource(bicyclepumporcompressedair)e1-heliumtanke1-rollofstringe12–balloonclipseIRTuborthefollowing:eReamofpapere1-extrablackinkcartridge(IRPrintertub)e1-extracolorinkcartridge(IRPrintertub) TheTheoryofpHpHTitrationCurvez8-magneticstirrerz8-magneticstirbarsz8-pHmeterswithelectrodesz3-extraelectrodes(pHtub)z1-500mLofpH4buffer(pHtub)z1-500mLofpH7buffer(pHtub)z8-boxeskimwipes(pHtub)z8-250mLbeakerz8-100mLgraduatedcylinderz8-50mLburetsz8-buretclampsz2-2Lbottlesof0.1MNaOHz1-2LbottleofunknownHClz8-washbottleszfilledpHcupsz1-pipetteboxz8smallfunnels(pHtub)Preparationofsolutions:z0.1MNaOH-dissolve4gofNaOHin1LofwaterzUnknownHCl-(0.1M)-dilute8.6mLofconc.HClto1L.NFPAInformation:ChemicalHealthFireReactivityNaOH302HCl300 TheTheoryofpHpHTitration:OxalicAcidz8-pHmeterwithelectrodesz12-250mLbeakersz8-100mLgraduatedcylindersz8-buretsz8-washbottlesz8-boxesofkimwipes(pHtub)z1-bottleofoxalicacid-50-100gz4-5-balancesz8-spatulasz50-weighboatsz8-magneticstirrersz8-magneticstirbarsz3-extrapHelectrodes(pHtub)z2-2Lbottlesof0.25MNaOHz1-500mLofpH4buffer(pHtub)z1-500mLofpH7buffer(pHtub)zfilledpHcups(pHtub)z8-smallfunnelsPreparationofsolutions:z0.25MNaOH-dissolve10gofNaOHin1Lofwater.NFPAInformation:ChemicalHealthFireReactivityNaOH302OxalicAcid311 TheTheoryofpHAnalysisofDrainCleanersz8-pHmeterswithelectrodesz3-extraelectrodes(pHtub)z1-carboyofDE-IONIZEDwaterz2-3-balancesz1-500mLofpH10buffer(pHtub)z1-500mLofpH7buffer(pHtub)z8-magneticstirrerz8-magneticstirbarszpipetteboxz8-50mLburetz8-buretclampz8-250mLbeakersz8-boxesofkimwipes(pHtub)z3-4-differentkindsofdraincleanersz2-2Lbottlesof0.10MHClzfilledpHcups(pHtub)z8-smallfunnels(pHtub)Preparationofsolutions:z0.10MHCl-dilute8.6mLofconcentratedHClto1LwithDE-IONIZEDwater.NFPAInformation:ChemicalHealthFireReactivityHCl300 TheTheoryofpHAceticAcidinVinegarz8-pHmetersz3-extraelectrodes(pHtub)z3-4-analyticalbalancesz1-500mLofpH10buffer(pHtub)z1-500mLofpH7buffer(pHtub)z8-buretclampsz8–50mLburetsz8-100mLbeakerz8-250mLbeakerz8-magneticstirrersz8-magneticstirbarsz1-pipetteboxz2-bottlesofvinegarz2-2Lbottlesof0.1MNaOHz8-boxesofkimwipes(pHtub)zfilledpHcups(pHtub)z8-smallfunnels(pHtub)Preparationofsolutions:z0.1MNaOH-dissolve4gofNaOHin1Lofwater.NFPAInformation:ChemicalHealthFireReactivityHCl300 TheTheoryofpHABufferz2-500mLbottlesof0.1Maceticacidz8-buretsz8-magneticstirrersz8-magneticstirbarsz1-pipetteboxz2-2Lbottlesof0.1MNaOHz8-pHmetersz3-extraelectrodes(pHtub)z8-boxesofkimwipes(pHtub)z1-500mLofpH4buffer(pHtub)z1-500mLofpH7buffer(pHtub)z8-buretclampsz8-100mLbeakerszfilled-pHcups(pHtub)z8-smallfunnels(pHtub)Preparationofsolutions:z0.1MNaOH-dissolve8gofNaOHin2LDE-IONIZEDwaterz0.1Maceticacid-dilute11.5mLofglacialaceticacidto2LwithDE-IONIZEDwaterNFPAInformation:ChemicalHealthFireReactiviyNaOH302AceticAcid221 TheTheoryofpHpHofHouseholdswith39Dropsz12–24-holeWellPlatesz8–lableddroppersforeachofthesolutions(including39Dropssolutions)z4–vialsofredlitmuspaperz4–vialsofbluelitmuspaperz8–20mLvialsofthefollowingsolutions:zAmmoniazAspirin(acetylsalicylicacid)andwaterzBakingpowderandwaterzBakingsodaandwaterzDIwaterzDranozPeroxidez7-upzRubbingalcoholzVinegarzUniversalIndicatorz1–1LbottleofSolutionAz1–1LbottleofSolutionBz8–30mLbottlesofsolutionAz8–30mLbottlesofSolutionBPreparationofSolutions:zSolutionA–5.96ganhydrousboricacidand9.6gcitricacidmonohydratedilutedto1LzSolutionB–37.6gtrisodiumphosphatedodecahydratedilutedto1LzSolidsinWater–dissolveonespatulaofthesolidinDIwater.NFPAInformation:ChemicalHealthFireReactivitySolutionA210SolutionB300 IdentificationLabsIdentificationofanUnknownGas¿30-largeballoons,numbered¿Listofmassesofballoons¿1-rollofstring¿availableanalyticalbalances¿8-thermometers¿8-rulers¿30–BalloonClips¿3–LargePlasticContainers¿1-gascylindersofthefollowinggases,asavailable:¿carbondioxide¿argon¿nitrogen¿oxygen¿helium¿hydrogenNFPAInformation:Noneneeded. IdentificationLabsMysterySolutions¿12–24-holeWellPlates¿WasteContainer¿8–bottlesofeachsolution¿0.1MHCl¿0.1MAgNO3¿0.1MNa2CO3¿0.1MNaCl¿0.1MPb(NO3)2¿0.1MKI¿8–bottlesofeachunknowninamberbottles¿A-0.1MHCl¿B-0.1MPb(NO3)2¿C-0.1MKI¿D-0.1MNaCl¿E-0.1MNa2CO3¿F-0.1MAgNO3¿LabeleddroppersforeachbottlePreparationofsolutions:¿0.1MHCl-Dilute4.3mLofconc.HClto500mLwithDE-IONIZEDwater¿0.1MNaCl-Dissolve2.9gofNaClin500mLofDE-IONIZEDwater¿0.1MAgNO3-Dissolve8.5gofAgNO3in500mLofDE-IONIZEDwater¿0.MNa2CO3–Dissolve5.3gofNa2CO3in500mLofDE-IONIZEDwater¿0.1MPb(NO3)2–Dissolve11.6gofPb(NO3)2in500mLofDE-IONIZEDwater¿0.1MKI–Dissolve8.3gofKIin500mLofDE-IONIZEDwaterNFPAInformation:ChemicalHealthFireReactivityHCl(A)300NaCl(D)200AgNO3(F)100Na2CO3(E)301Pb(NO3)2(B)303KI(C)200¿IdentificationLabs BloodTesting¿12–12-holeWellPlates¿Blood-stainedcloth–enoughpiecesforeachpairorperson¿Cryogenicvialsofbloodwithakey¿1–bagofextrasmallpipets¿6-8–dropperbottlesofantibodiesAandB¿Sendbottlesofbloodincasethedriverneedstorefill¿1boxofSmall,Medium,andLargeGloves¿30mLbeakers¿DIH2OWashBottles¿WasteBottlePreparationofsolutions:¿TypeA(1MNaOH)–4gNaOHdilutedto100mL.Addalittleredfooddyetocolor.¿TypeB(1MBaCl2)–21gBaCl2dilutedto100mL.Addalittleredfooddyetocolor.¿TypeAB(Sat.Ba(OH)2)–100mLwaterinbeakeronmagneticstirrer.AddBa(OH)2whilestirringuntilnomorewilldissolve.Addalittleredfooddyetocolor.¿TypeO(DIwater)–AddalittleredfooddyetoDIwater.¿AntibodyA(1MFeCl3)–16gFeCl3dilutedto100mL.Thesolutionwillbeyellow.Dothisinanicebathbecausethisgeneratesalotofheat!¿AntibodyB(1MNaCO3)–8gNaCO3dilutedto100mL.Add1dropbluefoodcoloring.**Note:Concentrationsneednotbeexact–allthatisnecessaryisthataprecipitateformswhenappropriatethingsreact.Youcouldmaketheseinabeakerifyoulike.ToStainCloth:Placeclothinaplasticcontainer.Pourabloodtypeonthecloth(usuallynottypeO)andthenplacetheclothinthecontainerinthefumehoodtoevaporate.NFPAInformation:ChemicalHealthFireReactivityTypeA(NaOH)300TypeB(BaCl2)200TypeAB(Ba(OH)2)200AntiA(FeCl3)302AntiB(NaCO3)301⇌ChemicalEquilibrium UsingLeChatelier’sPrinciple⇌1–bagsmalltesttubes⇌10–10mLgraduatedcylinder⇌10–smalltesttuberacks⇌10-stirringrods⇌10–60mLbottlesofthefollowing:⇌0.2Mcobaltchloride⇌0.1Msilvernitrate⇌concentratedHCl⇌acetonePreparationofsolutions:⇌0.2MCobaltchloride-dissolve4.8gofCoCl2•6H2Oin100mLofDE-IONIZEDwater⇌0.1MSilvernitrate-dissolve1.7gofsilvernitratein100mLofDE-IONIZEDwaterNFPAInformation:ChemicalHealthFireReactivitycobaltchloride200silvernitrate100hydrochloricacid300acetone230 ChemicalEquilibriumKSPofCopper(II)Tartrate⇌availablespectrophotometers⇌20–25mLErlenmeyerflasks⇌4-boxesofemptycuvettes(SpecTub)⇌8-automaticpipettesandtips(PipetBox)⇌12–10mLvolumetricflasks⇌1–bagofthin-stemmedpipettes⇌4–250mLbottlesof0.100Mcopper(II)sulfate⇌4–250mLbottlesof0.100Msodiumtartrate⇌centrifuge⇌8–centrifugetubes⇌1–WasteBottle(coppersulfate)Preparationofsolutions:⇌0.100Mcopper(II)sulfate-dissolve24.97gofcupricsulfatepentahydratein1LofDE-IONIZEDwater⇌0.100Msodiumtartrate-dissolve23.0gofsodiumtartratedihydratein1LofDE-IONIZEDwaterNFPAInformation:ChemicalHealthFireReactivitycoppersulfate200sodiumtartrate000 ChemicalEquilibriumKineticStudyoftheReactionofKIwithK2S2O8⇌availablespectrophotometers⇌Pipettebox(autopipetsandtips)⇌3-boxesofemptycuvettes(SpecTub)⇌1–bagofsmalltesttubes⇌DIwaterinwashbottlesandcarboy⇌8–60mLbottlesof0.020MKI⇌8–60mLbottlesof0.020MK2S2O8⇌1–bottleofremainingKIandK2S2O8solutions**Note:Thesolutionsmustbemadefresh.Preparationofsolutions:⇌0.020MKI–3.32gKIdilutedto1LwithDIwater.⇌0.020MK2S2O8–5.40gK2S2O8(potassiumpersulfate)dilutedto1LwithDIwater.NFPAInformation:ChemicalHealthFireReactivitypotassiumiodide200potassiumpersulfate100 ComputerLabsMeltingPointofSalol:12–LargeTestTubesfilledwithaninchofSalol:12–400mLor600mLBeakers:12–HotPlates:12–LaptopswithVernierSoftware:3–PrintersforLaptops:12–TemperatureProbes:12–SerialBoxInterfacesNFPAInformation:ChemicalHealthFireReactivitySalol210 DemosrationsLiquidNitrogenBananaHammerShrinkingBalloonsShatteringBallRubberHoseLiquidOxygenAirConditionerCondensingOxygenFrozenPetalsCloudNineSuperconductivityMethaneTorchDryIceBalloonBubblesinAquariumMagnesiumSparklerpHChangesCarbonDioxideStairsDiffusionofCarbonDioxideCarbonDioxideShowerColorDemonstrationsRainbowTubeIodineClockTrafficLightBlueBottleLuminolOscillatingReactionAmmoniaFountainBadBreathDisappearingInkElectrolyticCellsinSeries(PatrioticElectrolysis)OldNassauChemiluminescentFountainBZReactionLightDemonstrationsLightCordPersistenceofVisionMirrorKitWhiteLightGlowingHandsPictureStaringFlashBoardHumanLightBulbGlowingPickleFire/ExplosiveDemonstrationsMethanolCannonPolishCannonGunCottonLycopodiumPowderMethaneBubblesHydrogenBalloonHydrogenandOxygenBalloonEggsplosionThermiteBarkingDogsFlameThrowerKMnO4andGlycerinSugar,KClO3andH2SO4ZincandSulfurMiniFireworksVaporRampDancingFlame(BigCannon)UnderwaterFireworksProductionofHydrogenHydrogenBubblesColoredFlamesBurningMagnesiumGenieinaBottleFireandIceMethaneCanRepeatingExplodingFlaskVolcanoContactExplosive(Brownie)PolymersSlimeSillyPuttyRubberBallNylon6-10WaterLockMiscellaneousMemoryWireEndothermicReactionSodiumAcetateTreesReactionofMagnesiumandIodine“Silver”and“Gold”PenniesOxidationStatesofManganese Fire/ExplosiveDemonstrationsMethanolCannonM1–methanolcannon(1L)M1–methanolcannonprojectile(stopper)M1–250mLbottleofmethanolM1–TeslacoilPreparationofCannon–InserttwonailsthroughthesidesofaNalgenebottle.Thepointsofthenailsshouldbeseparatedbyabout1/4inchtoprovideagap.LittleCannonM1–littlecannonM1–littlecannonprojectile(styrofoamcups)M1–250mLbottleofethanolM1–lighter/packofmatchesM1–bagofthin-stemmedorjumbopipetsPreparationofCannon--Preparethecannonbycuttingoffthetopandhalfofthebottomofonesodacan.Cutthetopandthebottomhalfoppositetothefirstcanoffofasecondcan.Cutthetopoffofathirdcan,andpunctureasmallholeinthesidenearthebottom(seefigurebelow).Useducktapetofastenthecanstogether(putthefirstcanatthetopandthethirdcanatthebottom).Lycopodium M1–containeroflycopodiumM1–candleonastandM1–lighter/packofmatchesMethaneBubblesM1–methanebubbleaparatusM1–methanetankM1–methaneregulatorM1–2mstickwithacandleM1–lighter/packofmatchesM1–largewrenchM1–1Lbottleof3%DawnDishSoapM1–ringstandM1–ironringPreparationof3%DawnDishSoap–Dilute30mLofDawnDishSoapto1LwithDIwater.PreparationofAparatus--Cutoffthetop6"ofa2Lsodabottleandstopperitwithaone-holedstopperfittedwithaglasstubeconnectedtoarubberhose.Besurethatthetubingfitsonthemethaneregulator. Fire/ExplosiveDemonstrations(cont.)GunCottonM1–bagofguncottonM1–bagofuntreatedcottonM1–candleonastandM1–pairofcrucibletongsM1–lighter/packofmatchesPreparationofGunCotton--1.Cutthecottonintopieces2inchesby1inch.Completelysubmergeeachpieceofcottonintheacidmixture.2.Place210mLofconcentratedsulfuricacidina1.5–2Lbeaker,andputthebeakerinanicebath.3.Wetyourarmsandhandswell.Thispreventsburns.Slowlyadd90mLofconcentratedNitricacidwhilestirring.Thebeakerwillbefumingatthispoint.4.Addcottontothebeaker,completelysubmergingitintheacidmixture.5.Letthebeakerstandforonehour.6.Takethebeakeroutoftheicebath,andfillthebasinwithwater.7.Usingthetongs,placethecottoninthebasinofwaterandstiruntilallthepiecesareloose.8.Emptythisbasinofwaterandrepeat.Thendumpandaddfresh.9.Nextturnthewateronhighallowittoflowoverthecottonforaminuteortwo.Youneedtorinsethecottonseveraltimestoremoveexcessacid!10.Finallysqueezealltheexcesswaterfromthecottonandallowitdryovernightinthehoodonpapertowels.HydrogenBalloonM1–bagofmulti-coloredlargeballoonsM1–2mstickwithcandleM1–pairofheadphonesM1–lighter/packofmatchesM1–hydrogentankM1–rollofstringHydrogenandOxygenBalloonM1–bagofredballoonsM1–2mstickM1–packofpapertowelsM1–rolloflabelingtapeM1–pairofheadphonesM1–lighter/packofmatchesM1–hydrogentankM1–oxygentankM1–rollofstring Fire/ExplosiveDemostrations(cont.)BarkingDogsM1–1LglassgraduatedcylinderM1–250mLglassgraduatedcylinderM1–100mLglassgraduatedcylinderM1–50mLglassgraduatedcylinderM1–20mLvialofphosphorus/carbondisulfidesolutionM1–boxof7cmfilterpaperM1–boxof5.5cmfilterpaperM1–bagofjumbopipetsPreparationofphosphorus/carbondisulfidesolution--Cut~4gofwhitephosphorous(underwater),anddissolveitcompletelyin20mLofcarbondisulfide.FlameThrowerM1–bagoflargePyrextesttubesM1–bagofwoodsplintsM1–FisherburnerwithtubingM1–ringstandM1–fire-safeclampM1–adapterclampM1–lighter/packofmatchesM2–20mLvialsofpotassiumchlorate(KClO3)KMnO4andGlycerinM2–20mLvialsofpotassiumpermanganaeM1–250mLbottleofglycerinM1–crystalizingdishM1–scoopulaSugar,KClO3andH2SO4M2–20mLvialsofpotassiumchlorateM2–20mLvialsofgranulatedsugarM1–asbestospadM1–30mLbottleofconcentratedsulfuricacidM1–bagofjumbopipetsM1–scoopulaZincandSulfurM1–combustionspoonM1–ironringM1–ringstandM1–asbestospadM1–lighter/packofmatchesM1–BunsenburnerM2–20mLvialsofmossyzincM1–20mLvialsofsulfurM1–boxof11cmfilterpaperM1–scoopulaMiniFireworksM1–bagofPyrextesttubesM1–250mLbottleofconcetrtatedH2SO4M1–250mLbottleofmethanolM2–20mLvialsofpotassiumpermanganateM1–scoopula Fire/ExplosiveDemonstrations(cont.)VaporRampM1–vaporrampM1–ringstandM1–ironringM1–lighter/packofmatchesM1–candleonastandM1–50mLbeakerM1–lighter/packofmatchesM1–250mLbottleofhexaneDancingFlame(BigCannon)M1–bigcannon(withastrontiumchloride)M1–250mLbottleofmethanolM1–bagofwoodsplintsM1–pairofheadphonesM1–lighter/packofmatchesM1–oxygentankUnderwaterFireworksM1–250mLErlenmeyerflaskM1–one-holedrubberstopperforflaskwithglasstubingM1–pieceofrubberhosewithglasstubingbenttoahookM1–1LgraduatedcylinderM1–1Lbottleofwater(ifnecessary)M1–500mLbottleof6.0MHClM1–500mLbottleofbleachM1–250mLbottleofcalciumcarbideM1–scoopulaM2–50mLgraduatedcylindersPreparationof6.0MHCl–Dilute258.6mLofconcentratedsulfuricacidto500mLwithDIwater.ProductionofHydrogenM1–bagofPyrextesttubesM1–250mLbottleof6.0MHClM2–20mLvialsofmossyzincM1–bagofwoodsplintsM1–lighter/packofmatches**Note–Magnesiumpowdermaybeusedinsteadofmossyzinc.Preparationof6.0MHCl--Dilute129.3mLofconcentratedsulfuricacidto250mLwithDIwater.HydrogenBubblesM1–hydrogentankM1–hydrogenregulatorM1–largewrenchM1–1Lbottleof3%DawnDishsoapM1–methanebubbleaparatusM1–2mstickwithcandleM1–lighter/packofmatchesPreparationof3%DawnDishSoap–Dilute30mLofDawnDishSoapto1LwithDIwater.PreparationofAparatus--Cutoffthetop6"ofa2Lsodabottleandstopperitwithaone-holedstopperfittedwithaglasstubeconnectedtoarubberhose.Besurethatthetubingfitsonthemethaneregulator.Fire/ExplosiveDemonstrations(cont.) ColoredFlamesM1–crystalizingdishwiththefollowingsalts:MstrontiumchlorideMsodiumchlorideMcopperchlorideMpotassiumchlorideM2–emptycrystalizingdishesM1–250mLbottleofmethanolM1–250mLbottleofethanolM1–pairofcrucibletongsM1–watchglasstofitoverdishesM1–lighter/packofmatchesPreparationofCrystalizingDishes–Placeenoughofthesaltinthedishtojustcoverthebottom.PutthedishinaZiplockbag.BurningMagnesiumM1–bagofmagnesiumribbonM1–BunsenburnerwithtubingM1–lighter/packofmatchesGenieinaBottleM1–boxofKimwipesM2–20mLvialsofmanganesedioxideM7–20oz.plasticsodabottles(1/show)M1–rollofstringM1–500mLbottleofhydrogenperoxideM1–rubberstoppertofitbottlesFireandIceM1–bottleoflighterfluidM1–100mLbeakerM1–lighter/packofmatchesM1–500mLbottleofwater(ifnecessary)M1–cooleroficeMethaneCanM1–1gallonpaintcanwithholeM1–methanetankM1–3ftpieceofrubbertubingM1–lighter/packofmatchesM1–2mstickwithcandlePreparationofPaintCan–Obtainaclean1gallonpaintcan.Drilla3/8”inchholeinthecenterofthelidaswellasthesideofthecan1”fromthebottom.VolcanoM1–500gbottleofammoniumdichromateM1–asbestospadM1–bagofmagnesiumribbonM1–FisherburnerwithtubingM1–lighter/packofmatchesM1–scoopula Fire/ExplosiveDemonstrations(cont.)NFPAInformation:ChemicalHealthFireReactivitymethanol130ethanol030lycopodium210guncotton-1-phosphorus/carbondisulfidesol’n442potassiumchlorate200potassiumpermanganate100glycerin110sulfuricacid302zinc,mossy111sulfur210hexane130HCl300calciumcarbide332strontiumchloride100sodiumchloride200copperchloride300potassiumchloride200magnesiumribbon132manganesedioxide301hydrogenperoxide301ammoniumdichromate211 PolymerDemonstrationsSlimeR1–2LbottleofPVAsolutionR1–2LbottleofBoraxsolutionR1–boxofplasticbaggiesPreparationofsolutionsPoly(vinylalcohol)solution–Heat1Lofdistilledwatertoatemperaturenottoexceed90ºConastirringhotplate.Dissolve40gofpoly(vinylalcohol)inthewater.Addafewdropsoffoodcoloring(noscientificvalue-foraestheticpurposesonly).Boraxsolution–Dissolve76gofNa2B4O7•10H2O(sodiumtetraborateorborax)in1Lofdistilledwater.Note:Thissolutionissaturated,soitwilltakesomeheatingtogetalloftheboraxtodissolve.SillyPuttyR1–2LbottleofboraxsolutionR2–bottlesofElmer’sglueR1–boxofplasticbaggies0PreparationofsolutionsBoraxsolution–Dissolve76gofNa2B4O7•10H2O(sodiumtetraborateorborax)in1Lofdistilledwater.Note:Thissolutionissaturated,soitwilltakesomeheatingtogetalloftheboraxtodissolve.RubberBallR1–250mLbottleofMethanolR1–250mLbottleofSodiumSilicateNylon6-10R1–bottleofNylonASolutionR1–bottleofNylonBSolutionR1–250mLbeakerR1–pairofforcepsR1–stirringrodR1–boxoflargeglovesR1–wastebottle(forSebacoylChloride)Preparationofsolutions0.5Mhexamethylenediamine–Dissolve15.0gH2N(CH2)6NH2plus5.0gNaOHin250mLdistilledwater.Hexamethylenediaminecanbedispensedbyplacingthereagentbottleinhotwateruntilsufficientsolidhasmeltedandcanbedecanted.WaterLock:R4–StyrofoamcupsR2–20mLvailsofWaterlockpowderR2–bottlesofdifferentfoodcoloringR1–1LbottleWater(ifnecessary)R1–bottleofNaClR1–stirringrodorscoopulaPolymerDemonstrations(cont.) NFPAInformation:ChemicalHealthFireReactivityPolyvinylAlcoholSolution120BoraxSolution200Methanol130SodiumSilicate300NylonSolutionA310SebacoylChloride312 MiscellaneousDemostrationsMemoryMetal]1–packageofmemorymetal]1–hotplate]1–250mLbeaker]1–1Lofwater(ifnecessary)EndothermicReaction]3–20mLvialsofbariumhydroxide]3–20mLvialsofammoniumchloride]1–100mLbeaker]1–1Lofwater(ifnecessary)]1–smallwoodblock]1–1wastecontainer(forbariumhydroxideandammoniumchloride)SodiumAcetateTrees]3–20mLvialsofsodiumacetatetrihydrate]1–125mLErlenmeyerflask]1–stoppertofitflask]1–1Lofwater(ifnecessary)]1–hotplate]1–rollofaluminumfoilReactionofMagnesiumandIodine]2–20mLvialsofgranularmagnesium]2–20mLvialsofiodinecrystals]1–bagofPyrextesttubes]1–ringstand]1–adapterclamp]1–testtubeholder]1–Ironring]1–1Lofwater(ifnecessary)“Silver”and“Gold”Pennies]7–pre1982pennies(1perclass)]2–20mLvialsofzincmetal]1–evaporatingdish]1–1Lof3MNaOH]1–hotplate]1–Bunsenburner]1–pairoftongsPreparationofSolution3MNaOH–dissolve120gofNaOHin1LofDIwaterMiscellaneousDemonstrations(cont.) OxidationStatesofManganese]6–smallbeakers]1–bagofjumbopipets]1–100mLbottleofthefollowingsolutions:]SolutionA]SolutionB]SolutionC]SolutionD]SolutionE]SolutionFPreparationofSolutions:]SolutionA--Dissolve2gofMnSO4in100mLofDIwater.]SolutionB–Dissolve0.1gofKMnO4in100mLofDIwater.]SolutionC–Dissolve2gofNa2SO3in100mLofDIwater.]SolutionD–Dissolve33mLofconcentratedH2SO4in100mLofDIwater.]SolutionE–Dissolve24gofNaOHin100mLofDIwater.]SolutionF–Dissolve100gofNaOHin100mLofDIwater.MagicSand]1–bagofmagicsand]1–containerofregularsand]2–100mLbeakers]2–150mLbeakers]1–packofpapertowels]1–1Lofwater(ifnecessary)NFPAInformation:ChemicalHealthFireReactivityBariumHydroxide200AmmoniumChloride200SodiumAcetateTrihydrate212GranularMagnesium132IodineCrystals301ZincMetal111NaOH300MnSO4200KMnO4100Na2SO3201H2SO4302 TheNon-BurningTowel·8-400or600mLbeakers·8-250mLbottlesofethylalcohol·8-250mLbottlesofdistilledwater·astackofPapertowel·8-Tongs·Marshmallow·12-sterilizedfoodsticks(toputthemarshmallowon)(tosterilizehavebioautoclavethem)·2-4packsofmatches·2-grilllightersNote:adollarbillmaybeusedinplaceofthepapertowel,butitwillnotburnlongenoughtotoastthemarshmallow!Note:foodsticksshouldbesterilizedeverytimetheycomebackifpossibleNFPAInformation:ChemicalHealthFireReactivityEthanol030 ElephantToothpaste·10-100mLgraduatedcylinders·10-10mLgraduatedcylinders·2-boxoflatexgloves·4-boxoffoodcoloring·10-125mLbottlesofdishwashingliquiddetergent·1-boxoftrashbags·1L(per2classes)-30%hydrogenperoxide·10-125mLbottlesofsaturatedKI(potassiumiodide)SaturatedKIsolution:Placeabeakerofsuitablesizeonaheatedstirringplateandadddistilledwater.Continually,butslowly,addKIuntilitslowlytonolongerdissolves.NFPAInformation:ChemicalHealthFireReactivityHydrogenPeroxide301PotammisumIodide(KI)200 FatContentinFoodsä8–400mLbeakersä8–25mLgraduatedcylindersä8–stirringrodsäavailalbletop-loadingbalancesägroundmeatsamplesä8–250mLbottlesofhexaneäavailableglasspetridishesä2–sharpiemarkersNFPAInformation:ChemicalHealthFireReactivityhexane130 TieDyingvFilledWashBottlesforavailablecolorsofdyesv100–RubberBandsvArticlestoTie-Dyev2–TieDyeTubswithDyeFixerv1–boxmediumglovesv1–boxlargeglovesv50–garbagebagswithhandlestotiev50–boxesPreparationofSolutions:vDyes–Dissolve~2Tbsp.ofdyein~500mLofChemicalWateroChemicalWater–Dissolve1½c.Urea,4tsp.Ludigol,and2tsp.WaterSoftnerin2LofWarmWatervTieDyeTubswithDyeFixer–Dissolve7½cupsofdyefixerin40Lofwarmwater pHTubv8-funnelsv8–pH7buffercupsv8–pH4buffercupsv8–pH10buffercupsv1–500mLBottleofpH4bufferv1–500mLBottleofpH7bufferv1–500mLBottleofpH10bufferv8–boxesofKimwipesv3–ExtraelectrodesNFPAInformation:ChemicalHealthFireReactivitypH4buffer200pH7buffer100ph10buffer100 DemosSo,themobileeducatorputs“Demos”onthecalendar,andyoulookbackinthedemosectionandsay,“AH!WhattheheckdoIprep?”Onewaytoapproachthisproblemistogiveacopyofthedemolisttothedriverandaskthemtohighlightthedemostheywant.Youcanalsousethelistbelow.Thefollowinglistconsistsofusualdemosthemobileeducatorwoulduse,andthesedemosalonewouldconstitutea30min.demoshow.YouwillfindthatnormallyamobileeducatorwillwantLiquidNitrogendemosalongwiththelistbelow.Ifthemobileeducatorisdoingaclassthatislongerthan45-50mins.,theywillprobablyrequestevenmoredemosthanthoselistedbelow,butthecalendarshouldindicatethat.Ifyouareunsure,though,pleasecallthemobileeducatortoconfirmthelengthoftheshow.Thefollowinglistcontainsthesedemos:BigCannonColoredFlamesFlameThrowerGenieinaBottleGlowingPickleGunCottonHumanLightBulbHydrogen&OxygenBalloonHydrogenBalloonLittleCannonLuminolLycopodiumMagicSandMemoryWireMethaneBubblesMethanolCannonVolcanoWaterLockHerearethethingsthatshouldbeinthetubs:GeneralSupplieso1–2mStickwithCandleo1–BagofThin-stemmedpipetso1–bagofWoodSplintso1–Candleon“Stand”o1–ExtraCandle(ifnecessary)o1–FisherBurnerwithTubingo1–HydrogenTanko1–largeWrencho1–MethaneTanko1–OxygenTanko1–pairofHeadPhoneso1–pairofsafetyglasseso1–pairofScissorso1–rollofDuckTapeo1–rollofLabelingTapeo1–rollofStringo1–stackofPaperTowelso1–TeslaCoilo2–booksofMatcheso2–Lighterso2–pairsofTongso2–Scoopulas ChemicalSupplieso1–1Lbottleof3%DawnSolutiono1–1LbottleofLuminolSolutionAo1–1LbottleofLuminolSolutionBo1–20mLvialofredfoodcoloringo1–20mLvialofyellowfoodcoloringo1–250mLbottleofAmmoniumDichromateo1–250mLbottleofEthanolo1–250mLbottleofplainsando1–250mLbottleofSodiumchlorideo1–500mLbottleof30%HydrogenPeroxideo1–500mLbottleofMethanolo1–bagofGunCottono1–bagofmagicsando1–bagofMagnesiumRibbono1–bagofregularcottono1–containerofLycopodiumo1–CrystalizingDishw/CopperChlorideo1–CrystalizingDishw/SodiumChlorideo1–CrystalizingDishw/StrontiumChlorideo1–CrystalizingDishwithPotassiumChlorideo1–envelopeofmemorywireso1–jarofPickles(w/enoughpicklesforthe#ofclasses)o2–20mLvialsofManganeseDioxideo2–20mLvialsofPotassiumChlorateo2–20mLvialsofWaterLockApparatusSupplieso1–bagofMulti-coloredBalloonso1–bagofRedBalloonso1–20oz.PlasticBottleperclasso1–400mLBeakero1–AsbestosPadso1–bagofPyrexTestTubeso1–BigCannono1–boxofKimwipeso1–CorkorStopper(tofit20oz.bottle)o1–Fire-safeClampo1–FlorenceFlasko1–HotPlateo1–IronRingo1–LittleCannono1–LittleCannonProjectileo1–MethaneBubblesApparatuso1–MethaneRegulatoro1–MethanolCannono1–MethanolCannonProjectileo1–PickleWireo1–WatchGlass(largeenoughtofitoveracrystalizingdish)o2–100mLBeakerso2–150mLBeakerso2–EmptyCrystalizingDisheso2–FluorescentLightBulbso2–RingStando4–SmallWhiteCups DryIceDemonstrationsBalloonT1–bagoflargeballoonsTdryiceBubblesinAquariumT1–aquariumT1–bottleofbubble-blowingsolutionTdryiceMagnesiumSparklerT2–20mLvialsofmagnesiumpowderT1–bagofmagnesiumturningsT1–bagofmagnesiumribbonT2–20mLvialsofpotassiumchlorateT1–FisherburnerwithtubingTdryicepHChangesT3–500mLgraduatedcylindersT1–20mLvialofuniversalindicatorT1–2Lbottleof0.1MNaOHT1–2Lbottleof0.1MHClT1–2Lbottleofwater(ifnecessary)TdryiceSolutionPreparation:T0.1MNaOH--dissolve8gofNaOHin2LofDE-IONIZEDwaterT0.1MHCl--dilute17.2mLofconcentratedHClin2LofDE-IONIZEDwaterCarbonDioxideStairsT1–stairsapparatusT5–100mLbeakersT4–teelightcandlesT1–lighterT1–packofmatchesTdryiceDiffusionofCarbonDioxideT2–free-standingcandlesT1–aquariumTdryiceCarbonDioxideShowerT1–showerapparatusT1–1Lbottleofwater(ifnecessary)T1–125mLbottleofDawndishsoapT1–plasticpetridishT1–125mLbottleofglycerinTdryiceNFPAInformation:ChemicalHFRChemicalHFRChemicalHFRdryice200Mgribbon132NaOH300Mgpowder132KClO3200HCl300Mgturnings132universalindicator230glycerin210 ColorDemonstrationsRainbowTube$1–longglasstube$2–stoppersforendsoftube$1–1Lbottleof0.1MHClw/universalindicator$1–125mLbottleofsaturatedNa2COSolutionPreparation:$0.1MHClw/universalindicator–dilute8.6mLofconcentratedHClto1LwithDIwater.Addseveraldropsofuniversalindicatortogetadeepredcolor.$saturatedsodiumcarbonate–dissolvesolidsodiumcarbonatein100mLofwateruntilnomorewilldissolveIodineClock$1–1LbottleofSolutionA$1–1LbottleofSolutionB$2–50mLgraduatedcyliners$8–250mLbeakers(elementarystudents)$8–stirringrods(elementarystudents)$1–250mLbeaker(highschoolstudents)**Note:SolutionBmustbeusedwithin1weekofitspreparationSolutionPreparation:$SolutionA–Dissolve4.3gofKIO3(potassiumiodate)in1LofDIwater.$SolutionB–1.Place800mLofDIwaterina1Lbeakerandheatinthemicrowavefor10mins.2.Makeapasteof4gofwater-soluablestarch(foriodometry)inasmallamountofwarmwater.3.Addthepastetothe800mLofwaterandboilfor5mins.moreinthemicrowave.4.Coolthesolutionuntilittouchable.5.Add0.2gofsodiummetabisulfite.6.Prepare1.0Msulfuricacidbydiluting5.5mLofconcentratedsulfuricacidto100mL7.Add5mLof1.0Msulfuricacid.8.Diluteto1L.$Testthesolutions!!Itshouldtake15–20secs.forthesolutiontoturnblue.AddmoreacidtoSolutionBifthereactionistooslow,ordiluteSolutionBifthereactionistoofast. ColorDemonstrations(cont.)TrafficLight$1–250mLErlenmeyerFlask$1–StopperforErlenmeyerFlask$1–1LbottleofSolutionA$1–125mLbottleof1%IndigoCarminesolution**Note:Demonstrationworksbestifthesolutionsarefresh.SolutionPreparation:$SolutionA-Mix3gofdextroseand5gofNaOHin250mLofdeionizedwater.$1%IndigoCarmine–dissolve1gofindigocarminesolidin100mLofDIwater.BlueBottle$1–250mLErlenmeyerFlask$1–StopperforErlenmeyerFlask$1–100mLbottleofmethyleneblueindicatorsolution$1–1LbottleofSolutionA**Note:Demonstrationworksbestifthesolutionsarefresh.SolutionPreparation:$SolutionA--Add26.66gofKOHto1Lofde-ionizedwater.Coolthesolutionandadd35.33gofglucose.$MethyleneBlueIndicatorSolution–dissolve1gofmethylenebluesolidin100mLofDIwater.Luminol$1–500mLFlorenceFlask$1–1LbottleofSolutionA$1–1LbottleofSolutionBSolutionPreparation:$SolutionA-Dilute100mLofbleachwithdistilledwaterto1L.$SolutionB–Prepare0.10MNaOHbydissolving4gofthesolidin1LofDIwater.Add0.46gofLuminol(3-aminophthalhydrazide)to1Lof0.10MNaOH. ColorDemonstrations(cont.)OscillatingReaction$1–magneticstirrer$1–Stirbar$1–400mLBeaker$1–1LofSolutionA$1–1LofSolutionB$1–1LofSolutionC$3–100mLGraduatedCylinders**Note:SolutionCmustbeusedwithin1weekofitspreparation.SolutionPreparation:$SolutionA–Dilute410mLof30%H2O2to1LwithDIwater.$SolutionB–Prepare2.0MH2SO4bydiluting11mLoftheconcentratedacidto100mL.Mix43gofKIO3and40.0mLof2.0MH2SO4ina1Lvolumetricflask,anddilute.$SolutionC–Add0.30gofsolublestarch(foriodometry)toasmallamountofDIH2Oandheatuntildissolved.Ina1LVolumetricFlask,addstarchsolution,15.5gofMalonicAcid,and3.4gofMnSO4.Dilute.AmmoniaFountain$1–1Lbottleofwater(ifnecessary)$1–125mLbottleof1%phenolpthalein$1–gascylinderofammonia$1–3Lround-bottomflask$1–ringstand$1–ironring$1–2-holedstopperfortheflask$1–1Lbeaker$1–bagofjumbopipets$1–pieceofglasstubingwithrubbertubingattached$1–clampforrubbertubingBadBreath$3–125Erlenmeyerflasks$1–bag/boxofstraws$1–1LbottleofSaturatedCa(OH)2$1–500mLbottleofPhenolRedIndicatorSolution$1–500mLbottleofBromothymolBlueIndicatorSolution$1–125mLbottleof6.0MNH3SolutionPreparation:$SaturatedCa(OH)2-dissolve2gofCa(OH)2solidin1Lofdistilledwater.Stirtodissolveasmuchaspossible,thenfiltertogiveaclearsolution.Tightlycapthissolution.$PhenolRedIndicatorSolution–Prepare0.01MNaOHbydissolving0.04gofsolidNaOHin100mLofDIwater.Place0.1gofphenolredin26mLof0.01MNaOH.Add224mLofwater.$BromothymolBlueIndicatorSolution-place0.1gofbromothymolbluein16mLof0.01MNaOH.Add234mLofwater.$6.0MNH3–Dilute40.5mLofconcentratedammoniato100mLwithDIwater.ColorDemonstrations(cont.) DisappearingInk$1–125mLbottleofInkSolution$1–smallspraybottleof1.0MNaOH$1–smallbrush$1–pieceofcloth,paper,orbunchofwhitesilkflowerSolutionPreparation:$InkSolution--Ina150mLbeaker,dissolve0.10gramsofthymolphthaleinin10mLofethanol.Add90mLofwater;theliquidwillbemilky.Add20dropsof1.0MNaOHsolutionandtheliquidwillturnblue.$1.0MNaOH–Dissolve4gofsolidNaOHin100mLofwater.OldNassau$1–150mLbeaker$1–250mLbeaker$1–400mLbeaker$1–glassstirringrod$1–1LbottleofSolutionA$1–1LbottleofSolutionB$1–1LbottleofSolutionCSolutionPreparation:$SolutionA-1.Place800mLofDIwaterina1Lbeakerandheatinthemicrowavefor10mins.2.Makeapasteof4gofwater-soluablestarch(foriodometry)inasmallamountofwarmwater.3.Addthepastetothe800mLofwaterandboilfor5mins.moreinthemicrowave.4.Coolthesolutionuntilitistouchable.5.Add15gofNaHSO3.6.Diluteto1L.$SolutionB-Dissolve3gHgCl2in1LDE-IONIZEDwater.$SolutionC-Dissolve15gKIO3in1LDE-IONIZEDwater.ChemiluminescentFountain$1–3Lround-bottomflask$1–ringstand$1–ironring$1–2-holedstopperfortheflask$1–1Lbeaker$1–bagofjumbopipets$1–pieceofglasstubingwithrubbertubingattached$1–clampforrubbertubing$1–1LbottleofSolutionA$1–1LbottleofSolutionBSolutionPreparation:$SolutionA-add4gofNa2CO3and0.2gofLuminolto500mLDE-IONIZEDwater.Allowtodissolve.Add24.0gofNaHCO3,0.5gof(NH4)2CO3and0.4gofCuSO4.Allowtodissolve.Diluteto1LwithDE-IONIZEDwater.$SolutionB-Dilute5mLof30%hydrogenperoxideto1LwithDE-IONIZEDwater.ColorDemonstrations(cont.)BZReaction$1–9cmPetriDish$1–100mLbeaker $1–bagofjumbopipets$1–100mLbottleofSolutionA$1–100mLbottleofSolutionB$1–100mLbottleofSolutionC$1–100mLbottleofSolutionD$1–100mLbottleofSolutionESolutionPreparation:$SolutionA–dissolve5gofNaBrO3in100mLDIwater.$SolutionB–dissolve10gofNaBrin100mLofDIwater.$SolutionC–dissolve10gofmalonicacidin100mLDIwater.$SolutionD–dilute33.3mLofcocentratedSulfuricacidto100mL(6MSolution).$SolutionE–(FerroinSolution)dissolve1.35gof1,10-phenanthroline(notthehydrochloride)and0.7gofFeSO4in100mLofDIwater.NFPAInformation:ChemicalHFRChemicalHFRHClw/indicator300bromothymolblueindicatorsol’n210sat.sodiumcarbonate301ammonia300IodineClock,Sol’nA201DisappearinInkSol’n330IodineClock,Sol’nB302NaOH300TrafficLight,Sol’nA310OldNassau,Sol’nA111indigocarmine100OldNassau,Sol’nB300BlueBottle,Sol’nA301OldNassau,Sol’nC201methyleneblueindicatorsol’n210ChemiluminescentFountain,Sol’nA301Luminol,Sol’nB300ChemiluminescentFountain,Sol’nB301OscillatingRxn,Sol’nA301BZRxn,Sol’nA200OscillatingRxn,Sol’nB302BZRxn,Sol’nB200OsicllatingRxn,Sol’nC210BZRxn,Sol’nC210phenolpthalein230BZRxn,Sol’nD302sat.calciumhydroxide200BZRxn,Sol’nE212phenolredindicatorsol’n210 LightDemonstrationsPersistenceofVision5–SmallMirrorsWhiteLight1–fanwithcageremoved1–bagofredlightsticks1–bagofbluelightsticks1–bagofgreenlightsticksGlowingHands1–600mLbeaker1–pieceofcloth1–bagofredlightsticks1–bagofbluelightsticks1–bagofgreenlightsticks1–razorbladePictureStaring1–PictureofAnythingFlashBoard1–BrightLight1–FlashBoardPreparationofFlashBoard–Add~12.5gofphosphorescentzincsulfidetoapintofwhitepaintandmixthoroughly.Evenlypaintboardwithtwocoatsofthepaint,dryinginbetween.HumanLightBulb2–FluorescentLightBulbs1–TeslaCoilGlowingPickle1–PickleWire1–jarofpickles1–ScoopulaPreparationofPickleWire–Removethe“female”endofanextensioncordandseparatethetwoparts.Exposeabout2”ofthewire.NFPAInformation:ChemicalHealthFireReactivityzincsulfide100 Slimev50–20mLvialsofPVAv50–20mLvialsofBoraxfilledonly½fullvavailableSnack-sizebaggies****Note–Eachstudentneedsonebaggie.SillyPuttyv50–20mLvialsofboraxsolutionv8–bottlesofgluev1–gallonbottleofglue(ifneeded)vfoodcoloringvfoodcoloringdroppersvavailableSnack-sizebaggies****Note–Eachstudentwillneedtwobaggies.PreparationofSolutions:vPolyvinylalcohol(PVA)–1.Beginheating1Lofdistilledwaterwithstirring.Donotexceed90C.2.Add40gofpolyvinylalcohol.3.Continueheatingandstirringuntilallofthesoliddissolves.4.Addafewdropsoffoodcoloringorasmallscoopoffooddye.vBoraxSolution–Add76gofborax(sodiumtetraborate)to1Lofdistilledwaterandheatthesolutionuntilthesolidisdissolved.NFPAInformation:ChemicalHealthFireReactivityPVA120Borax200