异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用

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1、异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用作者：徐文莉1，何俊1，曾因明1，庞庆丰【摘要】目的观察异氟醚(isoflurane,ISO)后处理对盲肠结扎穿孔术(cecalligationandpuncture,CLP)后大鼠急性肺损伤(acutelunginjury,ALI)的保护作用。方法32只SD雄性大鼠随机分为4组(每组n=8)：正常组(N组)，未行任何处理;假手术组(Sham组)，单纯行开腹关腹术;盲肠结扎穿孔术组(CLP组)，行CLP建立大鼠ALI模型;异氟醚组(ISO组)，建立CLP模型后4h腹腔注射液态异氟醚2ml/k

2、g。术后12h各组动物腹主动脉放血处死，右肺进行病理切片和肺湿/干比(W/D)测定，组织匀浆测超氧化物歧化酶(SOD)、丙二醛(MDA)和髓过氧化物酶(MPO)的含量;左肺行支气管肺泡灌洗，取上清液测总蛋白、肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)的含量。结果肺组织病理学检测结果显示CLP组大鼠肺组织损伤严重，ISO组肺组织损伤较轻。与N组比较，CLP组和ISO组中SOD含量降低(P<0.01)，其余检测指标均增加(P<0.01)。与CLP组比较，ISO组SOD和IL-10含量较高(P<0.01);其余检

3、测指标均低于CLP组(P<0.05或P<0.01)。结论在本实验条件下，大鼠腹腔注射液态ISO可发挥抗炎、抗氧化作用，对CLP所致大鼠ALI有部分保护作用。12【关键词】急性肺损伤;肺保护;异氟醚;后处理;盲肠结扎穿孔　　Abstract:ObjectiveToinvestigatetheprotectiveeffectsofisoflurane(ISO)post-treatmentonacutelunginjury(ALI)inducedbycecalligationandpuncture(CLP)inrats.Methods

4、32maleSprague-Dawley(SD)ratswererandomlydividedinto4groups(n=8each):normal(N)group,withnotreatment;Sham-operatedcontrol(Sham)group,withmereincisionandclosureoftheabdomen;cecalligationandpuncture(CLP)group,establishedasratmodelofinducedALI;andisoflurane(ISO)group,withintrap

5、eritonealinjectionof2ml/kgISO4hafterCLP.Theanimalsweresacrificedbyexsanguinationviatheabdominalaorta12hafterCLP.Therightlungswereremovedforpathologicaldissection;determinationoflungwet-to-dryweightratio(W/D);determinationofthecontentofsuperoxidedismutase(SOD),malondialdehy

6、de(MDA)andmyeloperoxidase(MPO)inthepulmonaryhomogenate.Followinglavagesoftheleftlungs,thesupernatantfromthebronchoalveolarlavagefluid(BALF)wasdeterminedforthetotalproteinlevel,thecontentoftumornecrosisfactor-α(TNF-α)andinterleukin-10(IL-10).ResultsThepathologicalresultssho

7、wedthatratsinCLPgrouphadseriouslunginjury,whiletheratsinISOgrouphadslight12injury.InbothCLPandISOgroups,theSODcontentdecreased(P<0.01)andtheotherparametersincreased(P<0.01),ascomparedtotheNgroup.TheSODandIL-10contentswerehigherinISOgroupthaninCLPgroup(P<0.01).Thec

8、ontentsofMDA,MPOandTNF-αandW/DwerelowerinISOgroupthaninCLPgroup(P<0.05orP<0.01).Con