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ID:14239843
大小:3.54 MB
页数:481页
时间:2018-07-27
《developmental biology protocols - rocky s. tuan and cecilia w. lo》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、MethodsinMolecularBiologyMethodsinMolecularBiologyTMTMVOLUME137DevelopmentalDevelopmentalBiologyBiologyProtocolsProtocolsVVolumeIIIolumeIIIEditedbyEditedbyRockyS.TuanRockyS.TuanCeciliaW.LoCeciliaW.LoHUMANAPRESSOverview31DevelopmentalBiologyProtocolsOverviewI
2、IIRockyS.TuanandCeciliaW.Lo1.IntroductionThemarriageofcellandmolecularbiologywithembryologyhasproducedremark-ableadvancesforthefieldofdevelopmentalbiology.InthisthirdvolumeofDevelop-mentalBiologyProtocols,contemporary,practicalmethodsarefirstpresentedforthea
3、nalysisandmanipulationofdevelopmentalgeneexpression.Toillustratehowsuchtechniques,aswellasproceduresofexperimentalembryologyincludingthosedescribedinthefirsttwovolumesoftheseries,maybeappliedinthestudyofdevelop-ment,apanoramiccollectionofexperimentalmodelsof
4、morphogenesis,development,andcellulardifferentiationaredetailed.Bothinvivoandinvitrosystemsareincluded.Thevolumeconcludeswithvariousexamplesofdevelopmentalmodelsofdiseasesandtheirmolecularbasis.2.ManipulationofDevelopmentalGeneExpressionandFunctionDrosophila
5、hasbeenandremainsoneofthemostversatilemodelsystemsforthemanipulationofdevelopmentalgeneexpression.Chapter2focusesonadescriptionoftheexperimentalapproachescurrentlyusedinectopicgeneexpressioninDrosophilatoexaminethefunctionofagivengeneinthedesiredtissue.Chapt
6、er3dealswiththeutilizationofthehighlyefficientFLP/FRTyeastsite-specificrecombinationsystemtogeneratesomaticandgermlineclonesinDrosophilaforphenotypicanalysisandscreening.Chapters3and4addressthemethodsusedtoaltergeneexpressionaswellasgenefunctioninanotherexpe
7、rimentallyhighlyaccessiblesystem,thedevelopingchickembryo.Chapter3describestheapplicationofantisenseoligonucleotidesto“knockdown”geneexpressioninsomiticstagechickembryos,whereasChapter4discusseshowfunctionalneutralizingmonoclonalantibodiesmaybeusedtoblockthe
8、activityofaspecificgeneproduct,N-cadherin,inthedevelopingchickembryoniclimbbud.3.AnalysisofGeneExpressionThefirststepinanalyzingthemolecularbasisofanydevelopmentaleventistocharacterizeandcompare
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