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1、中国人血液淋巴细胞免疫表型参考值调查来源:17baba.com时间:2007-10-06字体:[大中小]收藏我要投稿 文章出处:朱敏转载请注明出处【摘要】目的建立健康中国成人血液淋巴细胞免疫表型分析参考值。方法用SimultestIMK-Lymphocyte试剂盒的荧光抗体对102例健康中国成人血液进行染色,以FACSort流式细胞仪进行分析,Simulset程序获取数据并分析结果,SPSS软件进行统计。结果18~65岁健康中国成人淋巴细胞参考值范围CD+3细胞是50%~84%(955~2860/
2、μl),CD-3CD+19细胞为5%~18%(90~560/μl),CD+3CD+4细胞为27%~51%(550~1440/μl),CD+3CD+8细胞为15%~44%(320~1250/μl),CD-3、CD+16和/或CD+56细胞为7%~40%(150~1100/μl),CD+3CD+4/CD+3CD+8比值为0.71~2.87。随着年龄增长CD+3CD+4细胞略有升高,CD+3CD+8细胞略有降低。NK细胞在大年龄组女性低于男性,且有统计学差异(P<0.05)。与高加索人和美国人相比,中国人
3、的CD+3CD+8细胞和NK细胞(CD-3,CD+16和/或CD+56)的相对值(百分率)与绝对值均高,CD-3CD+19细胞和CD+3CD+4细胞仅相对值较低,但绝对值不少。CD+3CD+4/CD+3CD+8比值向低值漂移。结论种族、性别、年龄和环境因素可影响健康人淋巴细胞表型分布。ReferencevaluesonbloodlymphocyteimmunophenotypeinhealthyChineseadultZhuLihua,WangJianzhong,FirstHospital,Beij
4、ingMedicalUniversity,Beijing,100034【Abstruct】ObjectiveToestablishthereferencevaluesonbloodlymphocyteimmunophenotypeinhealthyChineseadults.MethodStainingwholebloodwithfluorescein-conjugatedmonoclonalantibodiessuppliedbysimultestTMIMKlymphocytekit,weanal
5、yzed102healthyChineseresidents(agerange18~65years)byFACSortflowcytometer,acquiredresultsbySimultestprogramme,andperformedstatisticalanalysisbySPSSsoftware.ResultsThe95%referencerangesinabsolutecountspermicroliterofwholeblood(percentageoflymphocytes)for
6、CD+3,CD+3CD+4,CD+3CD+8,CD-3CD+19,CD-3/CD+16and/orCD+56cellswere955to2860(50%to84%),550to1440(27%to51%),320to1250(15%to44%),90to560(5%to18%),150to1100(7%to40%)respectively.TheCD+3CD+4/CD+3CD+8ratiowas0.71to2.87.CD+3CD+4cellsincreasedandCD+3CD+8cellsdecr
7、easedslightlywithage.Womenwerecomparedtomeninoldagegroup,NKcells(CD-3/CD+16and/orCD+56)werelower(P<0.05).ComparedwithCaucasiansandAmericans,ChinesehadhigherpercentageandabsolutenumbersofCD+3CD+8andNKcells,buthadlowerpercentageofCD-3CD+19andCD+3CD+4cell
8、swithoutabsolutenumberchange.SoCD+3CD+4/CD+3CD+8ratioskewtothelowervalues.ConclusionTherace,ageandenvironmentcouldinfluencethereferencevalueoflymphocyteimmunophenotype.【Keywords】LymphocyteimmunophenotypeReferencevalueFlowcytometry淋巴细胞免疫