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1、两种根管冲洗方法联合不同冲洗液对粪肠球菌作用体外探究【摘要】目的比较两种根管冲洗方法联合不同的冲洗液对抑制粪肠球菌的效果。方法将58颗单根管离体正畸牙根管预备后消毒,随机选取2颗检查灭菌效果,剩余56颗根管预备后接种粪肠球菌21d,按照冲洗方法不同随机分为超声根管冲洗组以及人工注射器冲洗组,每种冲洗方法中按照冲洗液的不同分为17%EDTA+1%NaClO(A组)、17%EDTA+2.5%NaClO(B组)、5.25%NaClO(C组)、0.9%NaCl(D组阳性对照组),每组7个标本,分别于根管冲洗前、后进行细菌取样培养计数,并做统
2、计学分析。结果无论在超声根管冲洗组或是人工注射器冲洗组,冲洗后各组根管内粪肠球菌量均显著下降(P0.05);将超声根管冲洗法与人工注射器冲洗法相比较,发现在A组与C组中,两种方法有显著差异(P0.05)。结论17%+2.5%NaClO可有效清除根管内的粪肠球菌,其效果优于17%EDTA+1%NaClO与5.25%NaClO,后两者间无显著差异;超声根管冲洗去除粪肠球菌的能力优于人工注射器冲洗。【关键词】粪肠球菌;超声根管冲洗;人工注射器冲洗;NaClO;EDTAAntimicrobialefficacyoftwomethodsof1
3、7irrigationanddifferentendodonticirrigantsusinginvitromodelinfectedbyenterococcusfaecalisCHENJian-hong,LILing,TANGQian,etal.DepartmentofStomatology,ThethirdAffiliatedHospital,SunYatsenUniversity,Guangzhou510630,China【Abstract】ObjectiveTheobjectiveofthisstudywastoevalua
4、tetheantibacterialefficacyoftworootirrigatingmethodsanddifferentendodonticirrigantstodisinfecthumanrootcanalsthathadbeencontaminatedwithEnterococcusfaecalis.MethodsTherootcanalsoffifty-eightextractedpremolarteethwereinstrumentedandautoclaved,twoofthemweretransferredtos
5、terilebrothtoserveasnegativecontrols.Enterococcusfaecaliswasusedtocontaminatetherootcanalsoftherestofthesamplesfor21d,theteethwererandomlydividedintotwogroups:ultrasonicirrigationgroupandsyringeirrigationgroup,eachgrouprandomlyandequallydividedintofourgroupswithdiffere
6、ntrootcanalirrigations.17%EDTA+1%NaClOwasusedforgroup17A,while17%EDTA+2.5%NaClOforgroupB,5.25%NaClOforgroupC,0.9%NaClforgroupD(Positivecontrolgroup),Thebacteriasampleswerecollectedwithsterilepaperpointsbeforeandafterirrigation.TheCFUwascountedandanalyzed.ResultsThenumb
7、erofE.faecalisinrootcanalsineachofgroupswaseffectivelyreduced(P0.05);GroupAandgroupCweresignificantdifference(A:P0.05).Conclusion17%EDTA+2.5%NaOClirrigationhaspotentialantibactericialeffectonE.faecaliswithinrootcanals,theantibacterialefficacyofthisirrigationwasbetterth
8、an17%EDTA+1%NaClOirrigationand5.25%NaClOirrigation,whiletheotherirrigationshavenosignificantdifference.Themethodofthe
