激发波长和发射滤光片的选择

《激发波长和发射滤光片的选择》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Syngeneprovidesfullflexibilitythroughouttherangeofimagingsysems,withexcitationenergysourcesavailableforallfluorescent,colorimetricandchemifluorescentapplications.Thechartbelowliststhedyetogetherwiththeexcitationandemissionwavelengths.TheFiltercolumnsuggestswhichoftheSyngenefiltertypesis

2、thebestchoiceforthatapplication.Forfurtherinformation,pleaseseeTechnicalNote10(underTechnicalSupport/ApplicationNotes).Dye/StainExcitationrange(nm)Peaks(nm)ExcitationsourceEmissionrange(nm)Peaks(nm)Filter Chemiluminescencen/an/an/aSubstratedependent425,430,440,461,560

3、nce350-500440Blue500-650560SG CoomassieBlueWhitelightn/aWhitelightvisiblebluen/anoneFilmWhitelightn/aWhitelightvisiblebrownn/anoneSilverWhitelightn/aWhitelightvisibleorange/brownn/anone EthidiumBromide250-600302MidUV550-700595UVFluorescein/FITC220-330290MidUV480-620518SP425-525494Blue48

4、0-620518SGwtGFP300-500395MidorLongUV480-580508SP470Blue480-580508SGeGFP/rsGFP350-525488Blue470-600508SGHoechst33258300-400348MidorLongUV380-650450SPAlexa488425-540495Blue480-625520SGVistraGreen250-320ndMidUV480-620520SP410-540490Blue480-620520SGOregonGreen488200-350ndMidUV480-620524SP42

5、5-550496Blue480-620524SG SYBRGold250-400300MidUV500-670537SP400-530495Blue500-670537SGSYBRGreenI220-540290MidUV480-630520SP380LongUV480-630520SP497Blue480-630520SGSYBRGreenII250-370254ShortUV480-640520SP400-520497Blue480-640520SGSYBRSAFE250-350280MidUV500-700530SPGelStarnd300MidUVnd527/

6、532rnaSPnd493Bluend527/532rnaSG SYPROOrange250-360300MidUV500-700570SP380-600470Blue500-700570SGSYPRORed230-400300MidUV550-750630LP400-630550n/a   SYPRORuby250-350280MidUV535-750610UV350-525450Blue535-750610SG RadiantRed230-330300ShortorMidUV550-700630LP350-600495Blue550-700630SGRhodami

7、ne123425-550505Blue500-650533SGTexasRednd370MidUV570-750615LP500-630595n/a    ProQDiamondndndMidUV550-700580UV475-600555n/a   ProQEmerald300200-400280MidUV460-640530SPDeepPurple300-400ndLongUV520-700600UV 460-600530n/a   Filters:LP-longpass,SP-shortpass,UV–midpass(etbr),SG-need