激发波长和发射滤光片的选择

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1、Syngeneprovidesfullflexibilitythroughouttherangeofimagingsysems,withexcitationenergysourcesavailableforallfluorescent,colorimetricandchemifluorescentapplications.Thechartbelowliststhedyetogetherwiththeexcitationandemissionwavelengths.TheFiltercolumnsuggestswh

2、ichoftheSyngenefiltertypesisthebestchoiceforthatapplication.Forfurtherinformation,pleaseseeTechnicalNote10(underTechnicalSupport/ApplicationNotes).Dye/StainExcitationrange(nm)Peaks(nm)ExcitationsourceEmissionrange(nm)Peaks(nm)Filter Chemiluminescencen/an/an/a

3、Substratedependent425,430,440,461,560

4、0-600302MidUV550-700595UVFluorescein/FITC220-330290MidUV480-620518SP425-525494Blue480-620518SGwtGFP300-500395MidorLongUV480-580508SP470Blue480-580508SGeGFP/rsGFP350-525488Blue470-600508SGHoechst33258300-400348MidorLongUV380-650450SPAlexa488425-540495Blue480-6

5、25520SGVistraGreen250-320ndMidUV480-620520SP410-540490Blue480-620520SGOregonGreen488200-350ndMidUV480-620524SP425-550496Blue480-620524SG SYBRGold250-400300MidUV500-670537SP400-530495Blue500-670537SGSYBRGreenI220-540290MidUV480-630520SP380LongUV480-630520SP497

6、Blue480-630520SGSYBRGreenII250-370254ShortUV480-640520SP400-520497Blue480-640520SGSYBRSAFE250-350280MidUV500-700530SPGelStarnd300MidUVnd527/532rnaSPnd493Bluend527/532rnaSG SYPROOrange250-360300MidUV500-700570SP380-600470Blue500-700570SGSYPRORed230-400300MidUV

7、550-750630LP400-630550n/a   SYPRORuby250-350280MidUV535-750610UV350-525450Blue535-750610SG RadiantRed230-330300ShortorMidUV550-700630LP350-600495Blue550-700630SGRhodamine123425-550505Blue500-650533SGTexasRednd370MidUV570-750615LP500-630595n/a    ProQDiamondnd

8、ndMidUV550-700580UV475-600555n/a   ProQEmerald300200-400280MidUV460-640530SPDeepPurple300-400ndLongUV520-700600UV 460-600530n/a   Filters:LP-longpass,SP-shortpass,UV–midpass(etbr),SG-need