常用荧光染料的激发和发射波长.pdf

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1、染料名称Excitation(nm)Emission(nm)分子量备注信息ReactiveandconjugatedprobesHydroxycoumarin325386331SuccinimidylesterAminocoumarin350445330SuccinimidylesterMethoxycoumarin360410317SuccinimidylesterCascadeBlue(375);401423596HydrazidePacificBlue403455406MaleimidePacificOrange403551Luciferyellow

2、425528NBD466539294NBD-XR-Phycoerythrin480;565578240k(PE)PE-Cy5conjugates480;565;650670akaCychrome,R670,Tri-Color,QuantumRedPE-Cy7conjugates480;565;743767Red613480;565613PE-TexasRedPerCP490675PeridininchlorphyllproteinTruRed490,675695PerCP-Cy5.5conjugateFluorX494520587GEHealthcareF

3、luorescein495519389FITC;pHsensitiveBODIPY-FL503512TRITC547572444TRITCX-Rhodamine570576548XRITCLissamine570590RhodamineBTexasRed589615625SulfonylchlorideAllophycocyanin650660104k(APC)APC-Cy7conjugates650;755767PharRedAlexaFluor系列荧光染料AlexaFluor350343442410AlexaFluor4054014211028Alex

4、aFluor430434540702AlexaFluor488499519643QY0.92AlexaFluor500503525700AlexaFluor514517542714AlexaFluor532530555724QY0.61AlexaFluor5465615721079QY0.79AlexaFluor5555535681250QY0.1AlexaFluor568579603792QY0.69AlexaFluor594591618820QY0.66AlexaFluor6106106291285AlexaFluor6336326481200Alex

5、aFluor6476526681300QY0.33AlexaFluor6606636911100AlexaFluor6806807021150AlexaFluor7006967191400AlexaFluor7507527761300AlexaFluor7907828041750Cy系列荧光染料Cy2489506714QY0.12Cy3(512);550570;(615)767QY0.15Cy3B558572;(620)658QY0.67Cy3.5581594;(640)1102QY0.15Cy5(625);650670792QY0.28Cy5.56756

6、941128QY0.23Cy7743767818QY0.28核酸相关探针染料Hoechst33342343483616AT-selectiveDAPI345455AT-selectiveHoechst33258345478624AT-selectiveSYTOXBlue431480~400DNAChromomycinA3445575CG-selectiveMithramycin445575YOYO-14915091271EthidiumBromide493620394AcridineOrange503530/640DNA/RNASYTOXGreen5045

7、23~600DNAVitalstain,TOTO:CyanineDimerTOTO-1,TO-PRO-1509533TO-PRO:CyanineMonomerThiazoleOrange510530PropidiumIodide536617668.4(PI)LDS751543;590712;607472DNA(543ex/712em),RNA(590ex/607em)7-AAD5466477-aminoactinomycinD,CG-selectiveSYTOXOrange547570~500DNATOTO-3,TO-PRO-3642661DRAQ5647

8、681,697413usableexcitationdownto4