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1、荧光素名称激发波长(nm)发射波长(nm)备注1,5IAEDANS336490 1,8-ANS372480 4-Methylumbelliferone385502 5-carboxy-2,7-dichlorofluorescein504529 5-Carboxyfluorescein(5-FAM)492518 5-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)542568 5-FAM(5-Carboxyfluorescein)49
2、2518 5-HAT(HydroxyTryptamine)370-415520-540 5-HydroxyTryptamine(HAT)370-415520-540 5-ROX(carboxy-X-rhodamine)578567604591 5-TAMRA(5-Carboxytetramethylrhodamine)548542552568 6-Carboxyrhodamine6G518543 6-CR6G518543 6-JOE520548 7-Amino-4-methylcoumarin351430 7-AminoactinomycinD(7-AAD)54664
3、7 7-Hydroxy-4-methylcoumarin360449,455 9-Amino-6-chloro-2-methoxyacridine412,430471,474 ABQ344445 AcidFuchsin540630 ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474 AcridineOrange+DNA502526 AcridineOrange+RNA460650 AcridineOrange,bothDNA&RNA440-480520-650 AcridineRed455-600560-680
4、 AcridineYellow470550 Acriflavin436520 AcriflavinFeulgenSITSA355-425460 Aequorin(Photoprotein) 466PhotoproteinAFPs-AutoFluorescentProtein-(Quantum Biotechnologies)seesgGFP,sgBFPAlexaFluor350™346342442441 AlexaFluor430™431540 AlexaFluor488™495,492519,520 AlexaFluor532™531,532553,554 Al
5、exaFluor546™556,557572,573 AlexaFluor568™577,578603 AlexaFluor594™590,594617,618 AlexaFluor633™632650 AlexaFluor647™647666 AlexaFluor660™668698 AlexaFluor680™679702 AlizarinComplexon530-560,580580,624-645 AlizarinRed530-560580 Allophycocyanin(APC)630,645655,660 AMC,AMCA-S345445 AMCA(Ami
6、nomethylcoumarin)345347425444 AMCA-X353442 AminoactinomycinD555655 Aminocoumarin346350442445 Aminomethylcoumarin(AMCA)345347425444 AnilinBlue 600 Anthrocylstearate360-381446 APC(Allophycocyanin)630,645655,660 APC-Cy7625-650755 APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505
7、 AstrazonBrilliantRed4G500585 AstrazonOrangeR470540 AstrazonRed6B520595 AstrazonYellow7GLL450480 Atabrine436490 ATTO-TAG™CBQCA465560 ATTO-TAG™FQ486591 Auramine460550 AurophosphineG450580 Aurophosphine450-490515 BAO9(Bisaminophenyloxadiazole)365395 BCECF(highpH)492,503520,528 BC