生物反应器课件 bioreactor design select cell culture method

《生物反应器课件 bioreactor design select cell culture method》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、BioreactordesignSelectcellculturemethodContentIntroductionCellculturespecificationAgitationTemperaturecontrolSterilizationCIPBioreactordesignconsiderationsGoals:MaxgrowthormaxproductivityNeedstringentlymaintainedprocessconditionsAgitation(maxtransfer)HomogenousconditionBioreactordesignconsiderat

2、ionsRepeatabletransferofenergy,substrates,metabolites.-Goodmixingdependson:naturemediumrheologyofthebrothairflowrate/typeofaerationkindandnumberofimpellers/bafflesorganismbehaviour(shearstress)geometryofvessel(H/D)accuratespeedcontrolBioreactordesignconsiderationsBioreactordesignconsiderationsfo

3、rcellcultureTopdrivenagitatorMarineimpellersTipspeedupto1m/sNobafflesMicroporousspargerGasflowsupto0.1vvmHeadspaceaerationAspectratio=1atworkingvolumeCIP-abledesignBioreactordesignconsiderationsShapeASMEBottomsHemisphericalBottomsJacketDesignConventionalDimpledHalfPipePortsTri-Clamp,DN,NA-connec

4、t,IngoldType,FlangetypeBioreactordesignconsiderationsASMEHead(s)HemisphericalHead(s)AgitationSealsMechanicalFaceSealsLipSealsMagneticDrivesImpellersTurbineMarinePitchedBladeOthersAgitationAgitation-turbineimpellerForunaeratedwater@20Cinafullybaffledtankthefollowingformulaapplies:P=4.5x10-13*Di5*

5、N3*FDi=ImpellerDiameterinInchN=RPMofShaftF=One(1)foroneimpeller(1.8)fortwoimpellers(2.4)forthreeimpellersAgitation-turbineimpellerTipSpeedshouldalsobeconsideredbecauseoftendencytoshearcells.Donotexceed1m/sforcellcultureAnd5-6m/sformicrobialcultures.TemperaturecontrolTemperaturecontrolCoolingSyst

6、em:remove50to100Watts/LiterofVolume.HeatingSystems:One(1)degreeCperminutebetween25and45CSpargersRingTypeSingleOrificeSinteredFiltersMembrane(0.2/0.45micronporesize)UsuallymadeofTeflonAdvantages--ValidatableDisadvantages--RelativelyExpensiveBlindedbyWaterDepthCartridgesAdvantages--NotblindedbyWat

7、erRelativelyefficientDisadvantages--NoteasilyvalidatedIntegritytesting:W.I.T.orbubblepointSterilityConsiderationsKillingRatesDeadLegsFittingTypesPipingConsiderationsSterilityConsiderationsCriticalElementsofSteamSterilization