罗汉果叶茎根的抑菌活性组分的研究

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1、罗汉果叶茎根的抑菌活性组分的研究【摘要】　　目的通过以活性为导向的提取分离的方法，首次研究罗汉果全植株中的抑菌活性组分。方法通过提取得到罗汉果全植株各个部分—果实、叶、茎和根的提取物，然后运用一种快速颜色指示法和血琼脂法研究检测它们的抑菌活性。结果罗汉果的非果实部分及果实对于口腔细菌转糖链球菌S.mutans具有很强的抑菌活性。为了进一步研究并分离活性组分，将其粗提物通过AmberliteXAD树脂进行分离纯化，再通过同样的检测方法进行抗菌活性测定，对有活性的组分通过血琼脂法进行验证。结论罗汉果叶、茎和根（非果实部分）以及果实都具有较强的抑菌活性；罗汉果的非果实

2、部分具有进一步开发为抗菌药物和保健品的潜力。【关键词】罗汉果全植株;抗菌染色检测方法;AmberliteXAD树脂;抑菌活性　　Abstract:ObjectiveThisresearchintendedtostudyandidentifytheantibacterialactivityofLoHanKuoplant(Momordicagrosvenori)byextractingandpurifyingbioactivesubstancesfromvariouspartsofLoHanKuoplant.MethodsVariousextractsLoHanKu

3、ofruit,leaf,vine,androot,andtheantibacterialactivityoftheseextractsetricdetectionmethodandagarplating.ResultsExtractsofLoHanKuoallpartsexhibitedstrongantibacterialactivityagainsttheoralbacteria,S.mutans.Tofurtherisolatethebioactivefractions,theextractsberlitechromatographyandthepurif

4、iedfractionsethod.ConclusionTheexperimentaldatademonstratedthatextractsofLoHanKuoleaves,vines,fruitandrootsexhibitedstrongantibacterialactivities.ThisstudysuggeststhatvariouspartsofLoHanKuohavethepotentialtobeusedforpharmaceuticalpreparationsanddietarysupplementstotreatvarioushumanai

5、lments,suchasinfection.　　Keyordicagrosvenori,Colorimetricdetectionmethod，Amberlitechromatography,Antibacterialactivity　　1IntroductionThroughoutthehistoryofChinapeoplehaveusedaproveimmunitytoinfection.OneplantthathasbeenmonlyusedistheLoHanKuofruit(Momordicagrosvenori).LoHanKuofruitsar

6、esmall-sizedfruitsanyotherprovinces.TheLoHanKuofruithasbeenutilizedforcenturiesastraditionalmedicinetotreatsorethroatandcoughing.LoHanKuofruitsaretypicallyharvestedinlatefallanddriedimmediately.ThedriedLoHanGuofruitsareusedasptstoidentifytheactivepoundsfromLoHanKuofruitsadeoverinthee

7、arlyfifties.Thoseeffortsresultedintheidentificationofintensivesogrosides(mogrosideItoVI).Thesepoundsaretriterpenoids,oietyattachedtoachemicalstructurecalledmogrolgroup［1］.ThebiologicalactivitiesofLoHanGuofruitshavebeenstudied［2～5］.PreviousresearchdemonstratedLoHanKuofruitextracthadan

8、tibacteriala