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1、中国水稻科学(ChinJRiceSci),2014,28(2):125-131http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2014.02.003125水稻千粒重QTLqTGW1.1的验证张宏伟朱玉君陈玉宇陈俊宇黄得润庄杰云∗(中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,杭州310006;∗通讯联系人,EGmail:jz1803@hzcnc.com)ValidationofqTGW1.1,aQuantitativeTraitLocusfor1000GgrainWeightinRice(Oryzasat
2、ivaL.)ZHANGHongGwei,ZHUYuGjun,CHENYuGyu,CHENJunGyu,HUANGDeGrun,ZHUANGJieGyun∗(ChineseNationalCenterforRiceImprovement/StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou310006,China;∗Correspondingauthor,EGmail:jz1803@hzcncc.om)ZHANGHongwei,ZHUYujun,CHENYuyu,etal.
3、ValidationofqTGW1.1,aquantitativetraitlocusfor1000Ggrainweightinrice(OryzasativaL.).ChinJRiceSci,2014,28(2):125G131.Abstract:Thisstudywasconductedtovalidateaquantitativetraitlocus(QTL)for1000Ggrainweight(TGW)whichwaspreviouslymappedina3.7Mbregiononthelongarmofricechromosome1,andtolocate
4、thisQTLmoreprecisely.AplantcarryingaheterozygoussegmentextendingfromRM11448toRM11615wasselectedfromthe3/Milyang46BCZhenshan972F5population.TheresultingBC2F6andBC2F7populationswereassayedwithSSRmarkers.ThreeBC2F7plantswereidentified,carryingheterozygoussegmentscoveringtheintervalsRM11448
5、-RM11522,RM11448-RM11549andRM1232-RM11615,respectively.NonGrecombinanthomozygoteswereidentifiedfromtheBC2F8populations,fromwhichthreesetsofnearisogeniclines(NILs)intheBC2F8∶9generationwereproduced,consistingoftwoparentalgenotypesintheintervalsRM11448-RM11522,RM11448-RM11549andRM1232-RM1
6、1615,respectively.Fieldtrialswereconductedtomeasureheadingdate(HD),TGW,andgrainshapetraitsincludinggrainlengthandwidth.TwoGwayanalysisofvariancewasperformedtotestthephenotypicdifferencesbetweentwogenotypicgroupsineachNILsetusingSASprogram.NosignificantvariationswereobservedonTGWandgrain
7、shapeintheNILwhichwasheterogeneousintheintervalRM11448-RM11522,whereassignificantgenotypiceffectsweredetectedintheremainingtwoNILsinwhichtheenhancingalleleswereallderivedfromZhenshan97.ForHD,nosignificanteffectsweredetectedinthethreeNILs.Basedoncomparisonamonggenomiclocationsof