花生ssr标记与农艺性状的相关性

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1、作物学报ACTAAGRONOMICASINICA2014,40(7):11971204http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.01197花生SSR标记与农艺性状的相关性**周金超杨鑫雷崔顺立侯名语陈焕英穆国俊刘立峰华北作物种质资源教育部重点实验室/河北省作物种质资源重点实验室/河北农业大学,河北保定071001摘要:以农家品种四粒红和冀农黑3号构建的包

2、含有251个家系的重组自交系(RIL)群体为材料,在河北保定市和邯郸大名县两地进行表型鉴定,利用Pearson’s相关和逐步多元回归分析了花生农艺性状之间及其与标记的相关性。结果表明,多数农艺性状间存在显著或极显著相关,其中相关性最高的为单株生产力和单株仁重(r=0.970),其次是主茎高和第一侧枝长(r=0.918);77个SSR标记与18个农艺性状显著相关,每个性状相关标记数在2~16之间;14个SSR标记与13个农艺性状关联,解释的表型变异为5.2%~11.5%。以上结果为花生常规育种和分子标记辅助选择奠定了基础。关键词:花

3、生;重组自交系;农艺性状;SSR;相关CorrelationbetweenSSRMarkersandAgronomicTraitsinPeanut(ArachishypogaeaL.)*ZHOUJin-Chao,YANGXin-Lei,CUIShun-Li,HOUMing-Yu,CHENHuan-Ying,MUGuo-Jun,andLIU*Li-FengNorthChinaKeyLaboratoryforCropGermplasmResourcesofEducationMinistry/KeyLaboratoryofCropGer

4、mplasmResourcesofHebei/AgriculturalUniversityofHebei,Baoding071001,ChinaAbstract:Arecombinantinbredline(RIL)populationincluding251lines,derivedfromthecrossbetweenSilihongandJinonghei3wasusedtostudythecorrelationsbetweenSSRmarkersandagronomictraits.Thephenotypesewereev

5、aluatedintwolocationsofBaodingandHandan,HebeiProvince.Pearson’scorrelationandstepwisemultiplelinearregressionanalysiswereconductedbasedonSSRandphenotypingdata.Theresultsshowedthatthereweresignificantcorrelationsamong18agronomictraits,withthehighercorrelationsbetweenpo

6、dweightperplantandseedweightperplant(r=0.970),aswellastheheightofmainstemandlengthoffirstbranches(r=0.918).ThereexistedsignificantcorrelationbetweenSSRmarkerandagronomictraitwithaaverageof2–6markerscorrelatedwitheachagronomictrait.FourteenSSRmarkerswereassociatedwith1

7、3agronomictraitswithex-planinedphenotypicvariancesof5.2%–11.5%.Alltheselaidasolidfoundationforpeanutconventionalbreedingandmolecularmarker-assistedbreedingprograms.Keywords:Peanut;Recombinantinbredline;Agronomictraits;SSR;Correlation作物的许多农艺性状如产量、品质和抗逆性等selection,MAS)对

8、目标性状进行改良,可以加快育[2]大多属于数量性状,遗传基础复杂,易受环境影响。种进程,提高育种效率。众多分子标记技术中,简通过表型选择的传统育种方法改良农艺性状周期长,单序列重复标记技术(simplesequencerepeat,SSR)[1]选