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1、中国水稻科学(ChinJRiceSci),２０１３,２７(５):４５７－４６５http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１３．０５．００２４５７水稻磷转运蛋白OsPHT２;１在提高磷素利用率方面的作用史书林＃王丹凤＃颜彦张芳王化敦顾冕孙淑斌∗徐国华(南京农业大学资源与环境科学学院,南京２１００９５;＃共同第一作者;∗通讯联系人,EＧmail:sunshubin＠njau．edu．cn)FunctionofPhosphateTransporterOsPHT２;１inImpro
2、vingPhosphateUtilizationinRiceSHIShuＧlin＃,WANGDanＧfeng＃,YANYan,ZHANGFang,WANGHuaＧdun,GUMian,SUNShuＧbin∗,XUGuoＧhua(CollegeofResourcesandEnvironmentalScience,NanjingAgriculturalUniversity,Nanjing２１００９５,China;＃Theseauthorscontributedequallytothispaper;∗Correspondingauthor,
3、EＧmail:sunshubin＠njaue．duc．n)SHIShulin,WANGDanfeng,YANYan,etal．FunctionofphosphatetransporterOsPHT２;１inimprovingphosphateutilizationinrice．ChinJRiceSci,２０１３,２７(５):４５７Ｇ４６５．Abstract:TheuptakeanddistributionofPiinplantsrequiresmultiplePitransportsystemsthroughoutgrowthandd
4、evelopment．AputativelowＧaffinityphosphatetransportergene,OsPHT２;１,wasidentifiedbasedonthericegenomicdatabasesandbioinformaticsanalysis．TheexpressionpatternofOsPHT２;１inricewasinvestigatedandtheresultsshoweditsmRNAexpressedinleafmainlyandinrootlittlewiththeusingofRTＧPCR．I
5、naddition,OsPHT２;１inducedbyphosphatedeficiencyandlight．ByAgrobacteriumtumefacienstransformationwithanUbiquitin(Ubi)promoter,thefunctionofOsPHT２;１wastestedbyoverＧexpressioninrice．Comparedwithwildtype,thePiconcentrationsofOsPHT２;１ＧOeplantsincreasedby４０．９％－４８．５％inleaves,３５
6、．６％－５１．２％inrootsandthebiomassincreasedby２５．１％－３０．３％atPiＧsufficientlevels．ThePiconcentrationsinOsPHT２;１ＧOeplantsincreasedby５３．１％－７０．３％inleavesandthebiomassincreasedby２５．６％－２８．５％atPiＧdeficientlevels,whilethePiconcentrationshadnodifferenceinroots．Infieldtest,thetotalPconce
7、ntrationsofOsPHT２;１ＧOeplantssignificantlyincreasedinthetopthreeleavesandpanicleaxis．ItindicatedthatOsPHT２;１mightbeinvolvedinthePiaccumulationinleavesandPitranslocationinplants．Keywords:rice;phosphate;phosphatetransporter;OsPHT２;１;overＧexpression史书林,王丹凤,颜彦,等．水稻磷转运蛋白OsPHT
8、２;１在提高磷素利用率方面的作用．中国水稻科学,２０１３,２７(５):４５７Ｇ４６５．摘要:植物对磷的吸收与体内再分配需要多种磷酸盐转运系统共同作用.应用公开的植物基因组数据库和生物信息学分析确定了一个水稻低亲和磷酸盐转
