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1、作物学报ACTAAGRONOMICASINICA2016,42(4):600608http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00600导入外源玉米C4型NADP-ME基因对小麦光合效能的影响1,21,21,2,*2222王永霞杜新华许为钢齐学礼李艳王会伟胡琳12南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095;河南省农业科学院小麦研究所/河南省小麦生物学重点实验
2、室,河南郑州450002摘要:为研究玉米C4型NADP-ME基因对小麦光合特性的影响,以T3代转NADP-ME基因小麦株系10T(9)-1-1和10T(9)-225-4及其对照周麦23为试材,进行了分子特征鉴定、光合生理特性分析和单株产量性状调查,并对其作用机制进行了初步解析。结果表明,外源基因已整合到转基因小麦的基因组中,并能正确转录和翻译;与对照周麦23相比,两个转基因株系在4个测定时期旗叶NADP-ME酶活性均明显提高,而旗叶净光合速率(Pn)则明显下降;尤其在花后第7天,两个转基因株系的酶活性较对照分别提高1.33倍和1.13倍,Pn分别较对照下降
3、17.26%和10.35%;千粒重和籽粒产量较对照均显著下降。与对照周麦23相比,转基因株系10T(9)-225-4利用强光和同化CO2能力下降,光合效率下降;旗叶气孔张开率和气孔导度均显著下降;苹果酸含量降低了5.6%,丙酮酸含量则提高了17.1%;外施苹果酸可恢复其光合速率。以上研究结果表明,玉米C4型NADP-ME基因的导入降低了小麦的光合特性,苹果酸含量降低引起的气孔导度下降可能是导致转基因小麦光合效能降低的原因。关键词:转基因小麦;NADP苹果酸酶;净光合速率;气孔导度PhotosyntheticCharacteristicsofTransgen
4、icWheatExpressingMaizeC4-TypeNADP-MEGene1,21,21,2,*2222WANGYong-Xia,DUXin-Hua,XUWei-Gang,QIXue-Li,LIYan,WANGHui-Wei,andHULin12NanjingKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity,Nanjing210095,China;HenanProvincialLaboratoryofWheatBiology/WheatRe
5、searchInstitute,HenanAcademyofAgriculturalSciences,Zhengzhou450002,ChinaAbstract:ToexplorethephysiologicalcharacteristicsofthetransgenicwheatexpressingmaizeC4-typeNADP-ME,weintro-ducedNADP-MEintotheC3cropwheatbyusingparticlebombardmenttransformation.Twotransgenicwheatlines(10T(9)-
6、1-1,10T(9)-225-4)andparentalcontrol(Zhoumai23)wereusedtostudymolecularcharacteristicsandphotosynthesisproperty,torevealthemechanism.TheresultsshowedthattheNADP-MEsequencewasintegratedintowheatgenome,andthetranscriptionandtranslationwereexactlysameasexpect.TheenzymeactivityofNADP-M
7、Einflagleafintransgenicplantswereincreasedsignificantlythanuntransformedplants,forinstanceitwasincreased1.33and1.13timesonthe7thdayafterflowering.Netpho-tosyntheticrate(Pn)offlagleafintransgenicplantsobviouslydecreasedwhencomparedtotheuntransformedplants.Onthe7thdayafteranthesis,P
8、noftransgenicwheatdecreasedby17.2
