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《干旱胁迫对中粒种咖啡幼苗膜脂过氧化_抗氧化酶活性和渗透调节物质含量的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、热带作物学报2014，35（5）：944-949ChineseJournalofTropicalCrops干旱胁迫对中粒种咖啡幼苗膜脂过氧化、抗氧化酶活性和渗透调节物质含量的影响杨华庚1112，颜速亮，陈慧娟，邓志声1海南大学农学院，海南海口5702282中国热带农业科学院科技信息研究所，海南儋州571737摘要以苗龄约8个月的中粒种咖啡袋育苗为材料，测定3种干旱胁迫强度下咖啡幼苗叶片的生理指标变化，旨在了解其对干旱胁迫的生理响应。结果表明：在轻度干旱胁迫下（土壤相对含水量为55％~60％），与对照相比，中粒种咖啡幼苗叶片的可溶性蛋白、可
2、溶性糖、脯氨酸含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性极显著增加，而丙二醛（MDA）含量和相对电导率没有显著变化。但在中度干旱或重度干旱胁迫下（土壤相对含水量为40％~45％或25％~30％），可溶性蛋白含量、SOD、POD、CAT活性显著或极显著减少，而可溶性糖、脯氨酸的积累减缓，MDA含量和相对电导率迅速上升。在适度干旱胁迫下，中粒种咖啡幼苗可通过提高抗氧化酶活性和渗透调节能力来增强其对干旱胁迫的耐性。关键词中粒种咖啡；干旱胁迫；抗氧化作用；渗透调节；膜脂过氧化；质膜透性中图分类号S571.2文献
3、标识码AEffectsofDroughtStressonLipidPeroxidation,AntioxidantEnzymeActivitiesandOsmolyteContentinLeavesofCoffeacanephoraSeedlings1112YANGHuageng,YANSuliang,CHENHuijuan,DENGZhisheng1CollegeofAgronomy,HainanUniversity,Haikou,Hainan570228,China2InstituteofInformationandTechnolog
4、y,ChineseAcademyofTropicalAgriculturalSciences,Danzhou,Hainan571737,ChinaAbstractCoffeacanephoraseedlingswereusedfortheexperimentattheageofabout8months.Threelevelsofdroughtstresswereappliedinthepresentexperiment,andthephysiologicalindicesofcoffeeleavesweremeasuredtounders
5、tandthephysiologicalresponsestodroughtstress.Experimentalresultsshowedthatwhencoffeeseedlingswereexposedtomilddroughtstress（relativesoilwatercontentof55%to60%），comparedwiththecontrolgroup,solubleprotein,solublesugarandprolinecontents,SOD,PODandCATactivitiesincreasedsignif
6、icantly,whileMDAcontentsandrelativeelectricalconductivityhadnosignificantchange.Butundermoderateorseveredroughtstress（relativesoilwatercontentof40%to45%or25%to30%）,solubleproteincontent,SOD,PODandCATactivitiesreducedsignificantly,whilesolublesugarandprolineaccumulatedrela
7、tivelyslowly,andMDAcontent,relativeelectricalconductivityincreaserapidly.TheseresultsshowthatCoffeacanephoraseedlingshaveimproveddroughttolerancebyincreasingantioxidantenzymeactivitiesandosmoregulationabilityundersuitabledroughtstressconditions.KeywordCoffeacanephora；Drou
8、ghtstress；Antioxidationaction；Osmoregulation；Lipidperoxidation；Membranepermeabilitydoi10.3969/j.
