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时间:2018-04-15
《向日葵黄萎病菌不同培养型产毒能力和致病力分化》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中国油料作物学报2014,36(3):393-397ChineseJournalofOilCropSciencesdoi:10.7505/j.issn.1007-9084.2014.03.016向日葵黄萎病菌不同培养型产毒能力和致病力分化*任杰,张贵,曹雄,周洪友,景岚,赵君(内蒙古农业大学农学院,内蒙古呼和浩特,010018)摘要:为了明确向日葵黄萎病菌间毒素产生能力以及致病力存在的分化现象,本文选取5种不同培养型的向日葵黄萎病菌对其菌落生长速度、粗毒素含量、毒素致萎活性及致病力进行测定。结果表明,不
2、同培养型的黄萎病菌分生孢子的形态没有显著差异,但是菌株生长速度、粗毒素产生量及其致萎能力存在一定的差异。粗毒素产生能力高的菌株,其致萎能力也强,如菌株V33(产生大量微菌核)、V21(不产生微菌核)、V27(产生一定量的微菌核及气生菌丝)的粗毒素分泌量分别为0.36、0.34、0.23mg/mL,其72h的致萎指数为58.33、52.08、37.50。采用纸钵撕底蘸根法对供试菌株的致病力进行测定,结果表明,不同培养型黄萎病菌的致病力存在一定的分化现象。其中致病力最强的菌株是V33,病情指数为53.97;
3、其次是V21和V39(产生大量微菌核),其病情指数分别为45.91和43.35;菌株V27的致病力最弱,其病情指数为37.44。相关性分析结果表明,菌株致病力的强弱与其分泌的粗毒素2含量呈显著的正相关关系(R=0.8169,P<0.05),但与其菌落生长速度以及微菌核的数量没有相关性。向日葵黄萎病菌毒素的分泌量是决定其致病力分化的关键因子。关键词:向日葵;黄萎病菌;培养型;粗毒素产生;致病力中图分类号:S435.655文献标识码:A文章编号:1007-9084(2014)03-0393-05Crudet
4、oxinproductionabilityandpathogenicitydifferentiationonsunflowerVerticilliumdahliae*RENJie,ZHANGGui,CAOXiong,ZHOUHong-you,JINGLan,ZHAOJun(CollegeofAgronomy,InnerMongoliaAgriculturalUniversity,Hohhot,InnerMongolia,China,010018)Abstract:Inordertotestthediff
5、erentiationofthepathogenicityofsunflowerVerticilliumdahliae,thecolonialmorphology,growthspeed,crudetoxinproductionability,wiltingcapabilityandpathogenicityamongfivedifferentisolateswerestudied.Ourresultssuggestedthatthereweren’tsignificantdifferenceamong
6、testedisolatesontheconidiamorphology,however,significantdifferenceswereobservedonthegrowthspeed,crudetoxinproductiona-bilityandwiltingcapability.CrudetoxinproductionabilityofV33,V21andV27were0.36mg/mL,0.34mg/mL,0.23mg/mLandthecorrespondencewiltingindexwe
7、re58.33,52.08,37.50respectively.Pathogenicitycapacityoftestedisolateswasverifiedbyrootsdippingsystem,isolateV33,whichformedlargeamountmicrosclerotia,showedthehighestdiseaseindex53.97;followedbyV21withoutformedmicrosclerotia,andV39withmicrosclero-tiaforme
8、dinlargeamount,theirdiseaseindexwere45.91and43.35respectively.V27hadthelowestdiseasein-dex37.44.Meanwhile,astrongpositivecorrelationwasdetectedbetweenpathogenicityabilityandthecrudetoxin2productionability(R=0.8169,P<0.05);
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