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ID:9014498
大小:1.31 MB
页数:9页
时间:2018-04-15
《ssr标记辅助回交转育大豆7s球蛋白亚基致敏蛋白缺失新品系》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中国油料作物学报2014,36(1):001-009ChineseJournalofOilCropSciencesdoi:10.7505/j.issn.1007-9084.2014.01.001SSR标记辅助回交转育大豆7S球蛋白α-亚基致敏蛋白缺失新品系123111111拓云,霍彩琴,田福东,宋波,申丽威,魏晓双,国博闻,李文滨,刘珊珊(1.东北农业大学大豆生物学教育部重点实验室,农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨,150030;2.黑龙江禾田丰泽兴农科技开发有限公司,黑龙江哈尔滨,150000;3.内蒙古通辽市农业科学研究院,内蒙古通辽,02
2、8015)摘要:为快速培育7S球蛋白致敏蛋白α-亚基缺失的优质大豆新品系,以(α′+α)-亚基双缺失型材料日B为供体亲本,东农47为受体亲本,以杂交-回交转育方法为基础,结合SSR标记辅助遗传背景选择与主要农艺性状鉴定及品质分析,在BC2F4选育到7S球蛋白α-亚基缺失新品系Cb80。Cb80综合农艺性状优良,遗传背景回复率大于90%,其蛋白质及氨基酸总量为44.1%、41.95%,分别比轮回亲本东农47提高3.5和3.76个百分点。关键词:大豆;7S球蛋白;α-亚基缺失;SSR标记辅助背景选择;回交育种中图分类号:S565.103文献标识码:A文章编号:1007-9
3、084(2014)01-0001-09Soybean7S(β-conglycinin)α-subunitdeficiencylinesdevelopedbybackcrossingassistedbySSRmarkerbackgroundselection12311TUOYun,HUOCAI-qin,TIANFu-dong,SONGBo,SHENLi-wei,1111WEIXiao-shuang,GUOBo-wen,LIWen-bin,LIUShan-shan(1.KeyLaboratoryofSoybeanBiologyinChineseMinistryofEdu
4、cation,KeyLaboratoryofSoybeanBiologyandBreeding/GeneticsofChineseAgricultureMinistry,NortheastAgriculturalUniversity,Harbin150030,China;2.HeilongjiangHetianfengzeAgriculturalScienceandTechnologyDevelopmentCo.,Ltd.Harbin150000,China;3.AgricultureScienceInstituteofTongliaoCity,InnerMongol
5、ia,Tongliao028015,China)Abstract:UsingaJapanesevarietyRiBwith(α′+α)-subunitsdeficiencyof7Sglobulinasthedonorparent,andDongnong47withhigherproteinandoilcontentasrecurringparent,characteristicofα-subunitdeficiencywassuccessfullyinducedintoDongnong47bythemethods,whichcombinedwithSSRmarke
6、rassistanceandvaluesofagronomicandqualitytraitsbasedoncrossingandbackcrossing.Aneweliteα-null-containingmutantline(Cb80),showinggreaterthan90%recurringparentgenomecontent,withhigherproteinandoilcontenthadbeendevelopedinBC2F4.TheproteinandtotalaminoacidscontentsofCb80were44.1%and41.95%,w
7、hichwerehigherthantherecurringparentDongnong47whoseratioswereincreasedby3.5%and3.76%respectively.Keywords:Soybean;7Sglobulin;α-subunitnull;SSRmarkerassistedbackgroundselection;Backcrossing大豆(GlycinemaxL.Merr.)7S球蛋白(β-7S球蛋白的减少可导致11S球蛋白的补偿性增[3,4][4]conglycinin)是由α′-(72kD)、α-(70kD
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