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1、中国科技论文在线http://www.paper.edu.cnThePreparationofHierarchicallyPorousBioactiveGlass#andImmobilizationofLysozyme*WangHongsu,LuoRuiping,ZhouXinrui5(CollegeofFoodScienceandEngineering,JilinUniversity,Changchun130062,China)Abstract:Hierarchicallyporousbioactiveglass(notedasYMBG)wasprepared
2、bypinepollenforlysozymeimmobilizationviaintermolecularinteractions.ThemorphologyandporediameterdistributionofYMBGwerecharacterizedbyscanningelectronmicroscopy(SEM),transmittedelectronmicroscopy(TEM)andnitrogenadsorption-desorption.FTIR,UV,fluorescence,andcircular10dichroism(CD)studie
3、swereshowedthatlysozymemaintaineditsoriginalsecondarystructureintheprocessoflysozymeimmobilization.Andtheoptimalexperimentalconditionswereexplored.Thismaterialpossessedahighlysozymeimmobilizedamount(648±5.1mg/gsupport).Inaddition,itwasfoundthattheadsorptionprocesswassuitablefortheLan
4、gmuirisothermalmodel.Andtheactivityofimmobilizedlysozymestillkeptabout52%oftheoriginalenzyme;theuseofimmobilizedenzyme15wasdistinguished,andtheactivityremainedabout25%afterusing4timesrepeatedly.Keywords:Materialscience,Hierarchicallyporousbioactiveglass,Lysozyme,Characterization0Intr
5、oductionHere,wereportedanovelapproachtoimmobilizelysozyme.YMBGwerepreparedbypine[1-3]20pollen,andthenuseitasacarriertoimmobilizelysozymebyintermolecularinteractions.Theoptimumconditionsofimmobilizelysozyme,suchasinitialconcentration,pH,adsorptiontemperatureandthenumberofoscillators(R
6、PM),wereinvestigated.Theoptimumconditionswereobtainedandthenovelmaterialspossessahighlysozymeimmobilizedamount(648±5.1mg/gsupport).Theimmobilizedlysozymehasahighactivityandismaintainedatabout52%of25theoriginalenzyme,anditholdsahighutilizationrate,theactivityremainedabout25%afterusing
7、4times.1Materialsandmethods1.1MaterialsPinepollenpowderwaspurchasedfromcommerciallyavailable;lysozymeandP12330triblockcopolymer(Poly(ethyleneglycol)-block-poly(propylene)-poly(ethyleneglycol))wereobtainedfromUnitedStatesSigma;triethylphosphate(TEP)waspurchasedfromShanghaiGuoyaoGroup;
8、Calciumnitra
