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1、OrganicPhaseAmperometricBiosensorforDetectionofPesticidesOrganicPhaseAmperometricBiosensorforDetectionofPesticidesA.CIUCU*,C.CIUCU***UniversityofBucharest,FacultyofChemistry,DepartmentofAnalyticalChemistry,90-92PanduriAv.,sector5,76235,Bucharest,Romania,e-mail:
2、anton@chem.unibuc.ro**UniversityofBucharest,FacultyofPhysicsReceived:10thDecember,2001;Accepted10thJanuary,2002AbstractArapid,simple,andsensitiveacetylcholinesterase(AChE)amperometricbiosensorfordirectmeasurementoforganophosphoruscompounds(OPCs)inorganicsolvent
3、wasdeveloped.Thebiosensorswerebasedonagraphiteelectrodeandonthiocholine-hexacyanoferratereaction.Theenzyme(AChE)wasco-immobilizedwithanelectronmediator,PrussianBlue,onthesurfaceoftheelectrode.Theeffectoforganicsolventsonacetylcholinesteraseactivitywasestimatedi
4、nthepresenceofpolar(hydrophilic)andnon-polar(hydrophobic)organicsolventsintherangeof0.01-100%.TheabilityoftheAChEbiosensorstoassaypesticideswasdemonstratedbyquantitativedeterminationofdichlorvos,fenthionanddiazinoninethanol.TheassayallowsdeterminationofOPCsinsu
5、b-micromolarconcentrationrangeswithanoverallassaytimeof10minutes.Themethodallowsadaptingthebiosensorfordetectionofpesticidesinactualwatersamples,avoidingtheproceduresofevaporationandresolution.Keywords:biosensor,amperometric,acetylcholinesterase,pesticides1.Int
6、roductionPesticides(herbicides,fungicides,insecticides)arewidelyusedthroughouttheworld,andmillionsoftonsareusedeachyearinagriculture,medicineandindustry.Manyofthemarehighlytoxic,however,andtheiraccumulationinlivingorganismscanbethecauseofseriousdiseases.Because
7、similarcompoundswereproducedaspossiblenervepoisonsafurtherareaofapplicationisinthemilitary.ThedestructionofOPC-basedchemicalweaponsmandatedbyinternationalagreementsoraspartofroutineoperationsalsoleadstoproblemsinenvironmentalcontrolandprotection.Pollutantsofthi
8、stypearefoundtobepresentinmanysampledsoils,groundandwastewater’sstreams.Oneofthemostimportantpreventivemeasuresinthiscaseistorapidlydeterminethesourceofthepollutantandthemag
