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1、Ricepollenhybridincompatibilitycausedbyreciprocalgenelossofduplicatedgenesa,ba,ca,b,1YokoMizuta,YoshiakiHarushima,andNoriKurataabPlantGeneticsLaboratory,GeneticStrainsResearchCenter,NationalInstituteofGenetics,Mishima,Shizuoka411-8540,Japan;DepartmentofGenetics,cSchoolofLifeScien
2、ce,GraduateUniversityforAdvancedStudies(Sokendai),Mishima,Shizuoka411-8540,Japan;andTransdisciplinaryResearchIntegrationCenter,ResearchOrganizationofInformationandSystems,Mishima,Shizuoka411-8540,JapanEditedbyDetlefWeigel,TheMaxPlanckInstituteforDevelopmentalBiology,Tübingen,Germ
3、any,andapprovedOctober11,2010(receivedforreviewMarch16,2010)Geneticincompatibilityisabarriercontributingtospeciesisolationfactorsoftheparentsofthehybrid,anddetectionofinteractingandiscausedbygeneticinteractions.Wemadeawholegenomelociisthefirststeptowardisolationofthecausalgenes.In
4、thissurveyoftwo-wayinteractinglociactingwithinthegametophytestudy,detectionofthetwo-wayinteractionandpositionalcloningorzygoteusingindependencetestsofmarkersegregationsinanF2ofthedetectedinteractinglocipairwerecarriedoutusingthepopulationfromanintersubspecificcrossbetweenO.sativas
5、ub-samecross-combinationusedpreviouslyformappingrepro-speciesindicaandjaponica.Wedetectedonlyonereproduciblein-ductivebarriers(14).Evolutionaryanalysisofthecausalgeneswasteraction,andidentifiedparalogoushybridincompatibilitygenes,performedtoinvestigatetheestablishmentofthisgenetic
6、in-DOPPELGANGER1(DPL1)andDOPPELGANGER2(DPL2),byposi-compatibilityinrice.tionalcloning.IndependentdisruptionsofDPL1andDPL2occurredinindicaandjaponica,respectively.DPLsencodehighlyconserved,ResultsandDiscussionplant-specificsmallproteins(∼10kDa)andarehighlyexpressedinDetectionoftheI
7、nteractingLociCausingGeneticIncompatibility.Formatureanther.PollencarryingtwodefectiveDPLallelesbecamefinemappingofareproductivebarrierasasinglegeneticfac-nonfunctionalanddidnotgerminate,suggestinganessentialroletor,andunderstandingitsmolecularmechanismsofhybridin-forDPLsinpolleng
8、ermination.Althoughricehasmanyduplicated
