水稻恢复系8821花粉育性中性基因的鉴定

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1、水稻恢复系8821花粉育性中性基因的鉴定董庆磊，陈鹏飞，王思哲，吴锦文，刘向东（华南农业大学亚热带农业生物资源保护与利用国家重点实验室，广东广州510642）摘耍：制粳亚种间杂种F1具有强人的生物学优势，然而F1育性普遍偏低影响其产量优势发挥。花粉不育是引起釉粳杂种F1育性偏低的最重要原WZ-,主要山S-a.S-b和S-c等至少6个基因座位的互作引起，而各座位上的屮性基因有望克服其不育性，所以挖掘和利川花粉育性中性基因具有重耍意义。本文利用水稻恢复系品种8821分别与粳稻台中65及其携带有花粉不育基因的近等基因系杂交组呢F1,对其F1的花

2、粉育性进行观察，结果发现S・a、S-b互作的各杂种F1花粉育性与无互作的杂种F1比较，彼此差异不显著;进一步利用分子标记检测F2群体中的3种基因型分离比例，结果符合孟徳尔分离比例（1:2:1）,说明8821在S-a和S-b座位上同时携带有釉粳杂种F1花粉育性中性基因，该材料有望克服大部分的釉粳杂种不育性，值得进一步深入研究。关键词：水稻；花粉育性；中性基因；恢复系中图分类号:S326IdentiHcationontheneutralgenesforpollen-sterilityinricerestorerline8821DONGQing

3、lei,CHENPengfei,WANGSizhe,WUJinwen,LIUXiangdong(StateKeyL

4、seofitsgiganticfeatures.However,partialsterilityinthehybridshasbecomeamajorobstacleforusingtheheterosisincommercialproduction.Pollensterilityisoneofthemostimportantreasontocausethelowfertilityofindica-japonicahybridsFLAtleastSixlocicontrollingthepollensterilityofintcr-sub

5、spccifichybridshavebeenidentifiedandwasfoundneutralallelesateachlocusarcpotentialtoovercometheFlpollensterilityassociatedwiththelocusfrompreviousstudies・Therefore,exploitationandutilizationofneutralgenesareofsignificantimportance.Taichung65anditspollen-sterilitynear-isoge

6、niclineswereusedastestparentsfordevelopingtest-crossesandtheirFlpollenfertilitywasexaminedinthepresentstudy.TheresultsshowedthatthepollenfertilitywerenotsignificantdifferencesbetweenFlwithinteractioninS-aandS-bloeouandtheFlwithnointeractioninthesamelocous・Theplantswiththr

7、eedifferentgenotypesinF2werecheckedbyusingmolecularmarkersandwasdisplayedthatsegregationofthe3genotypesfollowedtheexpectedMendelianratio(1:2:1)intheF2populations.Evidentially,8821carriedtheneutralgenesatS-aandS-blocus,whicharepotentialtoovercomethemostlypollen-sterilityof

8、hybridsbetweenindicaandjaponicaandneedopentostudy.Keywords:rice;pollenfertility;neutralgenes;ric