桃小食心虫滞育过程中5种代谢酶活力变化

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1、桃小食心虫滞育过程中5种代谢酶活力变化摘要：为明确桃小食心虫越冬适应机制，室内利用试剂盒测定了不同滞育时间桃小食心虫滞育幼虫的海藻糖酶、山梨醇脱氢酶、己糖激酶、丙酮酸激酶和碱性磷酸酶的活力。结果表明，不同滞育时间桃小食心虫体内海藻糖酶、己糖激酶、丙酮酸激酶、碱性磷酸激酶活力差异显著，而山梨醇脱氢酶活力差异不显著。海藻糖酶活力在滞育初期最高，而后随着滞育时间的延长逐渐降低;己糖激酶活力在滞育初期较低，在滞育后期先迅速升高再迅速下降;丙酮酸激酶在滞育中期活力最高，而碱性磷酸激酶活力在滞育初期最高。这表明桃小食心虫在滞育过程中通过代

2、谢酶的调节，提高其越冬期间的抗寒能力，保证顺利越冬。关键词：桃小食心虫;越冬;滞育;代谢酶ActivityChangesofFiveMetabolicEnzymesinCarposinasasakiiMatsumuraduringDiapauseShandongAcademyofAgriculturalSciences/KeyLaboratoryforPlantVirologyofShandong，Jinan250100，China）AbstractToclarifythephysiologicaladaptationmech

3、anismsofoverwintering，theactivitiesof5metabolicenzymes，includingtrehalase，sorbitoldehydrogenase，hexokinase，pyruvatekinaseandalkalinephosphatase，ofdiapauselarvaeofCarposinasasakiiMatsumuraduringdifferentdiapausetimeweremeasuredinlaboratorybykit.Theresultsshowedthatth

4、eactivitiesoftrehalase，hexokinase，pyruvatekinase，alkalinephosphatasehadsignificantdifferencesbetweendifferentdiapausetime，whilethoseofsorbitoldehydrogenasehadnosignificantdifference.Thetrehalaseactivitywasthehighestinearlydiapause，thengraduallydecreasedwiththeprolon

5、gationofdiapausesduration.Thehexokinaseactivitywaslowerinearlydiapause，thenincreasedrapidlyanddecreasedrapidlyinlaterdiapause.Thepyruvatekinaseactivityreachedthehighestlevelinmid-diapause.Thehighestactivityofalkalinephosphataseappearedinearlydiapause.Theseindicatedt

6、hatCarposinasasakiiMatsumuraimprovedtheircoldtolerancebyregulatingmetabolicenzymesduringdiapausetoensureoverwinteringsmoothly.KeywordsCarposinasasakii;Overwintering;Diapause;Metabolicenzyme桃小食心虫（CarposinasasakiiMatsumura）属于鳞翅目（Lepidoptera）蛀果蛾科（Carposinidae），是我国北方果树生

7、产中危害最大、发生面积最普遍的食心虫类害虫[4]。桃小食心虫以老熟幼虫滞育越冬，在滞育期间生长发育几乎处于停滞状态，但是体内仍